| Project/Area Number |
24659055
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental pharmacy
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAGASE Hisamitsu 岐阜薬大, 教授 (40141395)
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| Co-Investigator(Renkei-kenkyūsha) |
HIROMORI Youhei 金城学院大学, 薬学部, 助教 (60515956)
YAMAGUCHI Toshiyuki 神奈川大学, 理学部, 特任教授 (10101106)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 環境毒性学 / 有機スズ / 核内受容体 / 付着生物 |
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| Research Abstract |
Recently, we found that marine anti-biofouling organotins acts as powerful agonists for retinoid X receptor (RXR) in vertebrates and snails. So, we hypothesize that organotins' anti-biofouling effect may be involved in RXR signaling pathway. Here, we identified and characterized RXR from two typical sessile organisms, Mytilus galloprovincialis (MG) and Balanus albicostatus (BA). We cloned middle fragments of RXR from their tissues. The predicted RXR fragment proteins are characterized by the overall domain structure of a typical nuclear receptor. Amino acid alignments indicate that the ligand-binding domain (LBD) of MG and BA share about 80% and 60% sequence similarity with human RXRalpha, respectively. Consistent with this, RXR in MG can activate the transcription of reporter genes in response to stimulation by 9-cis retinoic acid, but not that in BA. Our findings suggest that structural and functional features of RXR may be much different among sessile organisms.
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