| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We found found HABP4, NUDT5, HMGB1 as a new nucleolin binding molecule using Yeast two-hybrid technique. Surface plasmon-resonance analysis revealed that these molecules had a strong binding to rNUC284.Advanced glycosylation end-products (AGEs) are non-enzymatically glycosylated proteins that play an important role in several diseases and aging processes. Surface plasmon-resonance analysis revealed that nucleolin strongly associated with GCA-BSA and GOA-BSA.We found nucleolin is a receptor that allows macrophages to recognize toxic AGEs.Amyloid-beta peptide 1-42 (Aβ42) plays a key role in the neurotoxicity found in Alzheimer's disease. We found monomeric and fibril Aβ42 were phagocytosed by mouse microglial EOC2 cells. These results indicate that nucleolin is a receptor that allows microglia to recognize monomeric and fibril Aβ42.
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