Search for intracellular introduction method of anti-tumor substance using microbial infection receptor nucleolin.
Project/Area Number |
24659057
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Environmental pharmacy
|
Research Institution | Takasaki University of Health and Welfare (2014) Tokyo University of Pharmacy and Life Science (2012-2013) |
Principal Investigator |
HIRANO KAZUYA 高崎健康福祉大学, 薬学部, 教授 (80251221)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | ヌクレオリン / ツーハイブリッドシステム / ヌクレオリン結合分子 / AGE / βアミロイド / two-hybrid法 / タンパク質 |
Outline of Final Research Achievements |
We found found HABP4, NUDT5, HMGB1 as a new nucleolin binding molecule using Yeast two-hybrid technique. Surface plasmon-resonance analysis revealed that these molecules had a strong binding to rNUC284.Advanced glycosylation end-products (AGEs) are non-enzymatically glycosylated proteins that play an important role in several diseases and aging processes. Surface plasmon-resonance analysis revealed that nucleolin strongly associated with GCA-BSA and GOA-BSA.We found nucleolin is a receptor that allows macrophages to recognize toxic AGEs.Amyloid-beta peptide 1-42 (Aβ42) plays a key role in the neurotoxicity found in Alzheimer's disease. We found monomeric and fibril Aβ42 were phagocytosed by mouse microglial EOC2 cells. These results indicate that nucleolin is a receptor that allows microglia to recognize monomeric and fibril Aβ42.
|
Report
(4 results)
Research Products
(7 results)
-
-
-
-
-
[Presentation] ヌクレオリン結合タンパク質の探索 ~COPS5との結合性~
Author(s)
鈴木寛香、橋本兆棋、井口聡介、土屋七奈、上野洋和、 有賀健、米沢康平、渋谷大輝、平野和也、別府正敏
Organizer
日本薬学会生物系薬学部会 主催 第14回Pharmaco-Hematologyシンポジウム
Place of Presentation
日本薬学会 長井記念ホール (東京都渋谷区渋谷1-12-15)
Related Report
-
-