Regulation of glucose and lipid metabolism through specific receptor-responsive FGF19 protein
| Project/Area Number |
24659064
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Medical pharmacy
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| Research Institution | National Fisheries University (2013)
Tohoku University (2012) |
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Principal Investigator |
MIYATA Masaaki 独立行政法人水産大学校, その他部局等, 教授 (90239418)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | FGF19 / 脂肪性肝疾患 / 脂質代謝 / 細胞増殖 / FGF19受容体 / FGF受容体 / 細胞増殖作用 / 糖代謝 / FXR欠損マウス |
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| Research Abstract |
We tried to prepare mutant FGF19 protein (FGF19-7) that lacks the activity of cell proliferation. FGF19-7 treatment slightly decreased CYP7A1 mRNA levels in HepG2 cells. Treatment of mice with FGF19-7 did not stimulate the number of Ki-67 positive cells that is a marker of cell proliferation. Mice were fed a methionin- and choline- deficient (MCD) diet for 6 weeks and co-treated with FGF19 or FGF19-7 (40 nmol/kg) for 4 days. Serum ALT and hepatic triglyceride and free fatty acid levels were markedly increased in mice fed MCD diet. These levels were significantly decreased in the mice co-treated with FGF19 or FGF19-7 to a similar extent. These results suggest that treatment with FGF19-7 lacking the activity of cell proliferation protects against MCD diet-induced steatohepatitis.
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Report
(3 results)
Research Products
(20 results)
