Research Project
Grant-in-Aid for Challenging Exploratory Research
We tried to prepare mutant FGF19 protein (FGF19-7) that lacks the activity of cell proliferation. FGF19-7 treatment slightly decreased CYP7A1 mRNA levels in HepG2 cells. Treatment of mice with FGF19-7 did not stimulate the number of Ki-67 positive cells that is a marker of cell proliferation. Mice were fed a methionin- and choline- deficient (MCD) diet for 6 weeks and co-treated with FGF19 or FGF19-7 (40 nmol/kg) for 4 days. Serum ALT and hepatic triglyceride and free fatty acid levels were markedly increased in mice fed MCD diet. These levels were significantly decreased in the mice co-treated with FGF19 or FGF19-7 to a similar extent. These results suggest that treatment with FGF19-7 lacking the activity of cell proliferation protects against MCD diet-induced steatohepatitis.
All 2014 2013 2012 Other
All Journal Article (6 results) (of which Peer Reviewed: 2 results) Presentation (14 results) (of which Invited: 1 results)
Biochem. Biophys. Res. Commun.
Volume: 443 Pages: 477-482
Volume: 443 Issue: 2 Pages: 477-482
10.1016/j.bbrc.2013.11.126
Euro. J. Pharmacol.
Volume: 714 Issue: 1-3 Pages: 507-514
10.1016/j.ejphar.2013.06.036
Eur. J. Pharmacol
Volume: 697 Issue: 1-3 Pages: 132-138
10.1016/j.ejphar.2012.09.048
J. Steroid Biochem. Mol. Biol.
Volume: 132 Issue: 1-2 Pages: 41-47
10.1016/j.jsbmb.2012.04.008
The Lipid
Volume: 23 Pages: 266-271
http://med.m-review.co.jp/magazine/detaill/J07_23_3_46-51.html
