Biological application of micro-dissection techniques for scanning electron microscopy
| Project/Area Number |
24659081
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOGA Daisuke 新潟大学, 医歯学系, 講師 (30467071)
NAKAJIMA Masato 新潟大学, 医歯学系, 助教 (60588250)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 電子顕微鏡 / マイクロ・ナノデバイス / マニピュレーション / 立体構造 / 細胞 / 組織 / 解剖学 / 細胞・組織 |
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| Research Abstract |
In the present study, we introduced a microdissection technique which can be used in a stereoscopic scanning electron microscope (3D-SEM) and applied this technique to the study on the three-dimensional ultrastructure of cells and tissues.
We developed preparation methods suitable for dissecting samples by the two micro-manipulators in the 3D-SEM. We also used the improved manipulators with a needle, micro-scissors and micro-forceps. Using these micro-manipulators, we succeeded in dissecting some tissue samples(e.g, kidney, inner ear, etc) in a 3D-SEM. Thus, the SEM-dissection technique in a 3D-SEM is very useful for the morphological study on cells and tissues.
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Report
(3 results)
Research Products
(11 results)
