• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on the neuron-specific transcriptional regulator Dpf1 using transgenic zebrafish expressing neuronal tracers

Research Project

Project/Area Number 24659085
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKobe University

Principal Investigator

KIKKAWA Satoshi  神戸大学, 医学(系)研究科(研究院), 准教授 (90244681)

Co-Investigator(Renkei-kenkyūsha) TERASHIMA Toshio  神戸大学, 大学院医学研究科, 教授 (20101892)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsクロマチン再構成複合体 / 神経細胞分化
Outline of Final Research Achievements

We indentified a main dpf1 transcrypt, dpf1-002, in the zebrafish central nervous system and determined the nucleotide sequence and expression pattern in embryos. In addition, in the dpf1-tTA-GFP fish, the GFP transgene was transcrived from the start point different from that of dpf1-002, and the expression of endogenous dpf1-002 was not affected by the expression of the transgene. We intend functional inhibition using morpholino antisense oligonucleotides (MO) for dpf1-002, but unfortunately have not yet observed clear-cut developmental nerve defects in the injected embyros. Examination such as the coinjection of different MOs is necessary. The different gene targeting approach such ad genome editing may also be needed in future.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (2 results)

All 2014

All Presentation (2 results)

  • [Presentation] Functional analysis of the Reelin-Dab1 signal pathway in the developing zebrafish nervous system.2014

    • Author(s)
      Satoshi Kikkawa, Takayuki Sumimoto, Takuya Sumi, Yoshiaki Sakihama, Toshio Terashima
    • Organizer
      Society for Neuroscience Annual meeting 2014
    • Place of Presentation
      Washington, DC
    • Year and Date
      2014-11-15 – 2014-11-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゼブラフィッシュdpf1遺伝子の新規転写産物の解析2014

    • Author(s)
      鷲見拓哉、崎浜吉昭、寺島俊雄、吉川知志
    • Organizer
      第119回日本解剖学会全国学術集会
    • Place of Presentation
      栃木県下野市・自治医科大学
    • Related Report
      2013 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi