| Project/Area Number |
24659088
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
WAGURI Satoshi 福島県立医科大学, 医学部, 教授 (30244908)
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| Co-Investigator(Kenkyū-buntansha) |
UEMURA Takefumi 福島県立医科大学, 医学部, 講師 (80548925)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Dipeptidase1 / 癌細胞 / 膜ドメイン / 酸化ストレス |
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| Outline of Final Research Achievements |
Cellular localization and functions of dipeptidase 1 (DPEP1), a marker of colorectal cancer, were investigated in this study. In a HCC56 cell line that expresses DPEP1 at high levels, DPEP1 was found to be localized on plasma membrane domains containing GPI-anchored protein, and at the bottom of microvilli. Suppression of DPEP1 by RNAi experiment did not affect the cell growth, but caused a vulnerability to oxidative stress, suggesting that DPEP1 might be involved in the tolerance of cancer cells against oxidative stress. Therefore, this study proposes a new mechanism involving DPEP1, which could be a target of anticancer drug development.
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