The establishment of anterograde tracing label of vagus nerve terminal in mice.-Resolution of the onset of hiding deabetes-

• Project/Area Number: 24659091
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Showa University
• Principal Investigator: Funahashi Hisayuki 昭和大学, 医学部, 准教授 (20317514)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 神経標識

Outline of Final Research Achievements

The goal the present study is to clarify the onset mechanism of the hiding diabetes. Melanocortin-4 receptor(MC4-R) has been noticed as critical regulator of insulin secretion. Morphological and histochemical analysis on MC4-R GFP mice indicates that the possibilities that the DMV neurons directly project to B cell in the pancreas. The method to efficiently label the vagus nerve terminals with anterograde tracer has been examined. The interesting combination of the tracer conjugated with special fluorescence agent and high sensible detector “IVIS Imaging system” may produce not only time-saving of the work but also decrease of a number of mice sacrified has been found. The working condition of the tracer is problem to be solved in the future.

Research Products

• [Journal Article] 高酸素負荷新生仔ラットの脳障害モデルとしての有用性の検討 (2015)
  • Author(s): 佐藤千佳、舟橋久幸
  • Journal Title: 昭和学士会雑誌 Volume: 75 Pages: 450-457
  • NAID: 130005121287
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Static and Dynamic Hypergravity Responses of Osteoblasts and Osteoclasts in Medaka Scales (2013)
  • Author(s): Yano S, et al. (14番目)
  • Journal Title: Zoological Science Volume: 30 Issue: 3 Pages: 217-223
  • DOI: 10.2108/zsj.30.217
  • Peer Reviewed
• [Journal Article] Neuroanatomy of melanocortin-4 receptor pathway in the lateral hypothalamic area. (2012)
  • Author(s): Cui H.
  • Journal Title: J Comp Neurol Volume: 520(18) Issue: 18 Pages: 4168-83
  • DOI: 10.1002/cne.23145
  • Peer Reviewed
• [Journal Article] Shared features of S100B immunohistochemistry and cytochrome oxidase histochemistry in the ventroposterior thalamus and lateral habenula in neonatal rats. (2012)
  • Author(s): Muneoka K.
  • Journal Title: Int J Dev Neurosci Volume: 30(6) Issue: 6 Pages: 499-505
  • DOI: 10.1016/j.ijdevneu.2012.05.003
  • Peer Reviewed
• [Journal Article] 魚類のウロコを用いた評価系の開発と骨代謝研究への応用 (2012)
  • Author(s): 鈴木信雄
  • Journal Title: The Magnetics Society of Japan Volume: 7(4) Pages: 174-178
• [Journal Article] Determination of Calcium Sensing Receptor in the Scales of Goldfish and Induction of Its mRNA Expression by Acceleration Loading. (2012)
  • Author(s): Makiko Kakikawa
  • Journal Title: Biological Sciences in Space. Volume: 26 Pages: 26-31
  • Peer Reviewed
• [Presentation] 走査型及び透過型電子顕微鏡によるキンギョウロコの収納ポケットの形態観察 (2014)
  • Author(s): 舟橋久幸
  • Organizer: 日本臨床分子形態学会
  • Place of Presentation: TKP市ヶ谷カンファレンスセンター
  • Year and Date: 2014-10-17 – 2014-10-18
• [Presentation] 走査型電子顕微鏡の有用性 走査型電子顕微鏡によるキンギョウロコの収納ポケットの形態観察 (2013)
  • Author(s): 舟橋久幸
  • Organizer: 日本臨床分子形態学会
  • Place of Presentation: アクロス福岡
• [Presentation] キンギョの再生ウロコにおける隆起線形成リズム (2012)
  • Author(s): 黒田美翔
  • Organizer: 日本比較内分泌学会
  • Place of Presentation: 福井大学
• [Book] 動物の事典 (2016)
  • Author(s): 舟橋久幸
  • Total Pages: 3
  • Publisher: 朝倉書店