Development of microscope system for single fluorescent molecule imaging in vivo
| Project/Area Number |
24659092
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
YASUSHI Okada 独立行政法人理化学研究所, 生命システム研究センター, チームリーダー (50272430)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 1分子イメージング / ライブイメージング / ゼブラフィッシュ / 蛍光ライブイメージング / 蛍光顕微鏡 / 共焦点顕微鏡 / in vivo イメージング |
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| Research Abstract |
Since the development of the single molecule imaging technique in Japan, it has been applied to wide areas of researches including in vitro analysis of molecular motors and single molecule measurements of signal transduction at the plasma membrane. However, this method is based on the total internal reflection microscopy, which limited its application to the region closer than 100 nm from the cover glass surface. In this project, we have tried to break this limitation and to observe single molecule deep inside the cell. By the combination of a Nipow-type confocal microscope and a high sensitivity-low noise camera, we have successfully observe the dynamics of single molecule deep inside the cell. We have further applied this technique for the super resolution imaging deep inside the cell.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] Genetically encoded system to track histone modification in vivo.2013
Author(s)
Yuko Sato, Masanori Mukai, Jun Ueda, Michiko Muraki, Timothy J. Stasevich, Naoki Horikoshi, Tomoya Kujirai, Hiroaki Kita, Taisuke Kimura, Seiji Hira, Yasushi Okada, Yoko Hayashi-Takanaka, Chikashi Obuse, Hitoshi Kurumizaka, Atsuo Kawahara, Kazuo Yamagata, Naohito Nozaki and Hiroshi Kimura
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Journal Title
Scientific Reports
Volume: 3
Issue: 1
Pages: 2436-2436
DOI
Related Report
Peer Reviewed / Open Access
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