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Development of microscope system for single fluorescent molecule imaging in vivo

Research Project

Project/Area Number 24659092
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

YASUSHI Okada  独立行政法人理化学研究所, 生命システム研究センター, チームリーダー (50272430)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords1分子イメージング / ライブイメージング / ゼブラフィッシュ / 蛍光ライブイメージング / 蛍光顕微鏡 / 共焦点顕微鏡 / in vivo イメージング
Research Abstract

Since the development of the single molecule imaging technique in Japan, it has been applied to wide areas of researches including in vitro analysis of molecular motors and single molecule measurements of signal transduction at the plasma membrane. However, this method is based on the total internal reflection microscopy, which limited its application to the region closer than 100 nm from the cover glass surface. In this project, we have tried to break this limitation and to observe single molecule deep inside the cell. By the combination of a Nipow-type confocal microscope and a high sensitivity-low noise camera, we have successfully observe the dynamics of single molecule deep inside the cell. We have further applied this technique for the super resolution imaging deep inside the cell.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (11 results)

All 2014 2013 2012 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (8 results)

  • [Journal Article] Viscosity and drag force involved in organelle transport : Investigation of the fluctuation dissipation theorem2013

    • Author(s)
      K. Hayashi, C.G. Pack, M. K. Sato, K. Mouri, K. Kaizu, K. Takahashi and Y. Okada
    • Journal Title

      Euro Phys J E

      Volume: 36 Issue: 12 Pages: 136-136

    • DOI

      10.1140/epje/i2013-13136-6

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetically encoded system to track histone modification in vivo.2013

    • Author(s)
      Yuko Sato, Masanori Mukai, Jun Ueda, Michiko Muraki, Timothy J. Stasevich, Naoki Horikoshi, Tomoya Kujirai, Hiroaki Kita, Taisuke Kimura, Seiji Hira, Yasushi Okada, Yoko Hayashi-Takanaka, Chikashi Obuse, Hitoshi Kurumizaka, Atsuo Kawahara, Kazuo Yamagata, Naohito Nozaki and Hiroshi Kimura
    • Journal Title

      Scientific Reports

      Volume: 3 Issue: 1 Pages: 2436-2436

    • DOI

      10.1038/srep02436

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A spontaneously blinking fluorophore based on intramolecular spirocyclization for live-cell super-resolution imaging

    • Author(s)
      S. Uno, M. Kamiya, T. Yoshihara, K. Sugawara, K. Okabe, M. C. Tarham, H. Fujita, T. Funatsu, Y. Okada, S. Tobita and Y. Urano
    • Journal Title

      Nat Chem

      Volume: (in press)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 高速・広視野・高分解能ライブセルイメージングのための超解像顕微鏡開発2014

    • Author(s)
      岡田康志
    • Organizer
      日本顕微鏡学会第70回記念学術講演会
    • Place of Presentation
      幕張
    • Year and Date
      2014-05-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 超解像顕微鏡の現状と課題~補償光学への期待2014

    • Author(s)
      岡田康志
    • Organizer
      若手研究者による分野間連携研究プロジェクトシンポジウムすばる望遠鏡から顕微鏡へ
    • Place of Presentation
      東京
    • Year and Date
      2014-03-24
    • Related Report
      2013 Final Research Report
  • [Presentation] Imaging-based analyses of the intracellular navigation mechanism of molecular motor, kinesin2013

    • Author(s)
      Y. Okada
    • Organizer
      第36回日本分子生物学会シンポジウム
    • Place of Presentation
      神戸
    • Year and Date
      2013-12-03
    • Related Report
      2013 Final Research Report
  • [Presentation] Super-resolution live imaging of intracellular transport system2013

    • Author(s)
      Y. Okada
    • Organizer
      Super Imaging 2013
    • Place of Presentation
      浜松
    • Year and Date
      2013-12-02
    • Related Report
      2013 Final Research Report
  • [Presentation] Atomic mechanism for the intracellular navigation of molecular motor, kinesin2013

    • Author(s)
      Y. Okada
    • Organizer
      Joint Weizmann-MBI mechanobiology conference
    • Place of Presentation
      Singapore
    • Year and Date
      2013-10-21
    • Related Report
      2013 Final Research Report
  • [Presentation] 誘導放出制御法(STED)およびその他の手法を用いた超解像ライブイメージング2013

    • Author(s)
      岡田康志
    • Organizer
      バイオイメージング学会第22回学術集会シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2013-09-15
    • Related Report
      2013 Final Research Report
  • [Presentation] 細胞内物質輸送の制御機構解明を目指した超解像ライブイメージング手法の開発2012

    • Author(s)
      岡田康志
    • Organizer
      第2回クリニカルサミット
    • Place of Presentation
      東京
    • Year and Date
      2012-12-25
    • Related Report
      2013 Final Research Report
  • [Presentation] ライブセルイメージングのための超解像蛍光顕微鏡法2012

    • Author(s)
      岡田康志
    • Organizer
      日本バイオイメージング学会第21回学術集会シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2012-08-27
    • Related Report
      2013 Final Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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