Regulatory mechanism of dendritic spine morphology by microRNA
Project/Area Number |
24659093
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
General anatomy (including Histology/Embryology)
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Research Institution | Tokyo Metropolitan Institute of Medical Science |
Principal Investigator |
YAMAGATA Kanato 公益財団法人東京都医学総合研究所, 脳発達・神経再生研究分野, プロジェクトリーダー (20263262)
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Co-Investigator(Kenkyū-buntansha) |
TANAKA Hidekazu 立命館大学, 生命科学部, 教授 (70273638)
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Co-Investigator(Renkei-kenkyūsha) |
SUGIURA Hiroko 公益財団法人東京都医学総合研究所, 脳発達・神経再生研究分野, 主席基盤技術研究職員 (40162870)
YASUDA Shin 公益財団法人東京都医学総合研究所, 脳発達・神経再生研究分野, 研究員 (20392368)
|
Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
Keywords | マイクロRNA |
Outline of Final Research Achievements |
Various microRNAs (miRNAs) have been identified in the mammalian brain. However, there is few literature concerning how miRNA regulates dendritic spine morphology. Therefore, I tried to identify and investigate miRNA that binds to arc mRNA, which is induced by neuronal activity and localizes to dendritic spines. Overexpression of this miRNA caused thin spines in primary neurons probably by reducing arc protein levels. We next identified a ubiquitin ligase as one of arc-binding proteins. Reduction of this ubiquitin ligase also caused thin spines in neurons. Knockout of this ligase resulted in distinct post-translational modification of arc protein. These results suggest that decrement or different modification of arc protein may change dendritic spine morphology.
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Report
(5 results)
Research Products
(39 results)
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[Journal Article] Glucose-dependent acetylation of Rictor promotes targeted cancer therapy resistance.2015
Author(s)
Masui K, Tanaka K, Ikegami S, Villa GR, Yang H, Yong WH, Cloughesy TF, Yamagata K, Arai N, Cavenee WK, Mischel PS.
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Journal Title
Proc Natl Acad Sci U S A.
Volume: 112(30)
Issue: 30
Pages: 9406-9411
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Activation of Rheb, but not of mTORC1, impairs spine synapse morphogenesis in tuberous sclerosis complex.2014
Author(s)
Yasuda S, Sugiura H, Katsurabayashi S, Shimada T, Tanaka H, Takasaki K, Iwasaki K, Kobayashi T, Hino O, Yamagata K.
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Journal Title
Sci Rep.
Volume: 4
Issue: 1
Pages: 5155-5155
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Rheb activation disrupts spine synapse formation through accumulation of syntenin in tuberous sclerosis complex2015
Author(s)
Yasuda S, Sugiura H, Katsurabayashi S, Kawano H, Endo K, Takasaki K, Iwasaki K, Ichikawa M, Kobayashi T, Hino O, Yamagata K
Organizer
第58回日本神経化学会大会
Place of Presentation
大宮ソニックシティ(埼玉県さいたま市)
Year and Date
2015-09-12
Related Report
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[Presentation] Activation of Rheb, but not of mTORC1, impairs spine synapse morphogenesis in tuberous sclerosis complex.2014
Author(s)
Yasuda S, Sugiura H, Katsurabayashi S, Shimada T, Tanaka H, Takasaki K, Iwasaki K, Kobayashi T, Hino O, Yamagata K
Organizer
第36回日本生物学的精神医学会・第57回日本神経化学会大会合同年会
Place of Presentation
奈良県文化会館(奈良県奈良市)
Year and Date
2014-09-29 – 2014-10-01
Related Report
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