| Project/Area Number |
24659097
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | University of Fukui |
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Principal Investigator |
MITA Kenichiro 福井大学, 医学部附属病院, 特命助教 (30529342)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | イオンチャネル / 選択性 / 脂質平面膜法 / 単一チャネル電流 / チャネル病 / Kチャネル / イオン選択性 |
|---|
| Research Abstract |
We challenged to uncover the mechanism of the selectivity of the potassium channel. The channel currents of a mutant E71A was measured using the planar lipid bilayer method under a bi-ionic condition (extracellular: 200 mM KCl, intracellular: 200 mM NaCl or LiCl). Inward K+ currents were readily observed, while no outward Na+ or Li+ currents were detected. When the membrane potential was applied to flow K+, K+ currents were detected after a short latency. This indicates that the channel was blocked by Na+ or Li+ and released with latency. And with different membrane potentials, we elucidated that Na+ can enter the channel more easily than Li+.
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