The mechanism of the selectivity of potassium channels.
| Project/Area Number |
24659097
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General physiology
|
|---|
| Research Institution | University of Fukui |
|---|
Principal Investigator |
MITA Kenichiro 福井大学, 医学部附属病院, 特命助教 (30529342)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
|
|---|
| Keywords | イオンチャネル / 選択性 / 脂質平面膜法 / 単一チャネル電流 / チャネル病 / Kチャネル / イオン選択性 |
|---|
| Research Abstract |
We challenged to uncover the mechanism of the selectivity of the potassium channel. The channel currents of a mutant E71A was measured using the planar lipid bilayer method under a bi-ionic condition (extracellular: 200 mM KCl, intracellular: 200 mM NaCl or LiCl). Inward K+ currents were readily observed, while no outward Na+ or Li+ currents were detected. When the membrane potential was applied to flow K+, K+ currents were detected after a short latency. This indicates that the channel was blocked by Na+ or Li+ and released with latency. And with different membrane potentials, we elucidated that Na+ can enter the channel more easily than Li+.
|
Report
(3 results)
Research Products
(21 results)
-
-
[Journal Article] デジタル処理による動脈圧波形の歪みの補正2012
Author(s)
三田 建一郎, 佐藤 倫祥, 清水 久美子, 次田 佳代, 松木 悠佳, 田畑 麻里, 信川 泰成, 上田 雅史, 林 和子, 廣瀬 宗孝, 重見 研司
-
Journal Title
麻酔・集中治療とテクノロジー2009
Volume: 1
Pages: 56-59
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
