| Project/Area Number |
24659098
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAWAI Yoshiko 信州大学, 医学部, 准教授 (10362112)
NAGAI Takashi 信州大学, 医学部, 助教 (50514353)
AJIMA Kumiko 信州大学, 医学部, 助教 (70584051)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 病態生理 / センチネルリンパ節 / 微小環境 / 癌転移 |
|---|
| Research Abstract |
Metastasis of carcinoma cells mainly occurs through the lymphatic system, and the extent of metastasis in lymph nodes is clinically used as a useful prognostic indication. The clinical importance of SLN has been proven in many breast cancer patients. However, the biological and histological properties of lymphatic endothelial cells (LEC) in the SLN that interact with micrometastatic carcinoma cells remain unclear. Therefore, using the human breast carcinoma cell line MDA-MB-231, we have attempted to examine the effects of supernatants cultured with the cell line on the expression of adhesion molecules on human LEC and then to investigate whether the expressed adhesion molecules accelerate the attachment of carcinoma cells to the LEC. The experimental findings suggest that MDA-MB-231 may release or leak ATP, which produces the overexpression of ICAM-1 on human LEC through activation of purinergic receptors and then facilities ICAM-1-mediated attachment of carcinoma cells to the LEC.
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