| Project/Area Number |
24659100
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ISHIKAWA Yoshihiro 横山市立大学, 医学研究科, 教授 (40305470)
ICHIKAWA Yasuhiro 横山市立大学, 医学部, 助教 (10555121)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 循環器・高血圧 / 生理学 / ナノバイオ / 細胞・組織 / 三次元培養 / 弾性線維 / 動脈硬化 |
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| Research Abstract |
Elastic fiber formation is disrupted with age and by health conditions including aneurysms and atherosclerosis. Despite considerable progress in the understanding of elastogenesis using the planar culture system and genetically modified animals, it remains difficult to restore elastic fibers in diseased vessels. To further study the molecular mechanisms, we created in vitro three-dimensional vascular constructs. Three-dimensional cellular multilayers (3DCMs), which consisted of seven layers of neonatal rat aortic SMCs cultured in 1% fetal bovine serum in DMEM medium, exhibited layered elastic fibers and differentiated smooth muscle cell phenotype within seven days of being in a static culture condition. Furthermore, infiltration of THP-1-derived macrophages decreased the surrounding elastic fiber formation in 3DCMs. 3DCMs may offer a new experimental vascular model to explore pharmacological therapeutic strategies for abnormal vascular remodeling.
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