| Project/Area Number |
24659107
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MIZUKAMI Yoichi 山口大学, 大学研究推進機構, 教授 (80274158)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | RNAiコレクション / エストロゲン / シグナル伝達経路 |
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| Research Abstract |
Estrogen plays a role for variety of physiological functions such as cell growth and cell differentiation through transcriptional regulation by nuclear estrogen receptors. However, intracellular pathways such as calcium influx and NO production in early responses occurred by estrogen stimulation have been unclear. To elucidate the early responses for the cells by estrogen, we have carried out high-throughput negative screening assay using RNAi library of GPCRs and kinases.
To determine accurately, we developed automation assay by JANUS, robot assay workstation using 96-well plates, and the cell response by estrogen stimulation was measured using mitochondria membrane potential assay in the RNAi-trasnfected cells. In several GPCR genes, mitochondria activities were significantly inhibited, suggesting the estrogen-dependent cell growth may be regulated by plasma membrane GPCRs. However, the results were further confirmed by the additional experiments.
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