Analysis of mechanisms underlying strain recognition memory in mice using visualization techniques

• Project/Area Number: 24659108
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: Kochi University
• Principal Investigator: KABA Hideto 高知大学, 教育研究部医療学系, 教授 (50136371)
• Co-Investigator(Kenkyū-buntansha): OKUTANI Fumino 高知大学, 教育研究部医療学系, 准教授 (10194490) ; TANIGUCHI Mutsuo 高知大学, 教育研究部医療学系, 助教 (10304677) ; MURATA Yoshihiro 高知大学, 教育研究部医療学系, 助教 (40377031)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 匂い / 記憶 / 副嗅球 / 可視化 / 発火パターン / 代謝型グルタミン酸受容体 / Ca2+チャネル / バゾプレッシン / ドキシサイクリン / c-fosプロモーター / フェロモン / 交尾 / 僧帽細胞 / 顆粒細胞

Research Abstract

By using transgenic mice that stably express the green fluorescent protein (GFP) coupled to the activity-dependent gene c-fos in mitral and granule cells naturally activated by the association of mating male chemosignals and mating signal in the accessory olfactory bulb (AOB), we found in AOB slices that GFP-positive mitral cells fired in burst mode in contrast to GFP-negative mitral cells that fired in tonic mode. In addition, we found in AOB slices prepared from normal mice that the activation of mGluR2 on mitral cells in the AOB inhibited voltage-dependent Ca2+ channels, thereby suppressing the release of glutamate from the mitral cells and that vasopressin that enhances the induction of long-term potentiation at the mitral-to-granule cell synapse in the AOB reduced dendrodendritic inhibition of mitral cells.

Research Products

• [Journal Article] 社会的絆を支える匂いの記憶・学習のメカニズム (2013)
  • Author(s): 椛秀人
  • Journal Title: 分子精神医学 Volume: 13 Pages: 260-266
  • URL: http://www.sentan.com
• [Journal Article] Regulation of synaptic cur rents by mGluR2 at reciprocal syna pses in the mouse accessory olfact ory bulb (2013)
  • Author(s): Taniguchi M, Yokoi M, Shinohara Y, Okutani F, Murata Y, Nakanishi S, Kaba H
  • Journal Title: European Journal of Neur oscience Volume: 37 Issue: 3 Pages: 351-358
  • DOI: 10.1111/ejn.12059
  • Peer Reviewed
• [Journal Article] 嗅覚 (2013)
  • Author(s): 椛秀人
  • Journal Title: Clinical Neuroscience Volume: 31 Pages: 70-72
  • URL: http://chugaiigaku.jp/
• [Journal Article] Neurobiology of secure infant attachm ent and attachment despite adversit y : a mouse model (2013)
  • Author(s): Roth TL, Raineki C, Salstein L, Perr y R, Sullivan-Wilson TA, Sloan A, La lji B, Hammock E, Wilson DA, Levitt P, Okutani F, Kaba H, Sullivan RM
  • Journal Title: Genes Brain and Behavior Volume: 12 Issue: 7 Pages: 673-680
  • DOI: 10.1111/gbb.12067
  • Peer Reviewed
• [Presentation] マウス副嗅球僧帽細胞—顆粒細胞間相反性シナプス伝達に対するバゾプレッシンの効果 (2014)
  • Author(s): 谷口睦男,難波利治,椛秀人
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス,鹿児島
• [Presentation] オスマウス副嗅球におけるシナプス可塑性に対するバゾプレッシンの役割 (2014)
  • Author(s): 難波利治,谷口睦男,村田芳博,奥谷文乃,椛秀人
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス,鹿児島
• [Presentation] マウス副嗅球シナプスの長期増強におけるアクチン重合化の関与 (2014)
  • Author(s): 村田芳博,椛秀人
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス,鹿児島
• [Presentation] フェロモン記憶と個体認知 (2013)
  • Author(s): 椛秀人,藤田博子,吾妻健,奥谷文乃,松波宏明
  • Organizer: 日本味と匂学会第47回大会嗅覚シンポジウム「化学シグナルと嗅覚行動および脳機能」
  • Place of Presentation: 仙台市民会館,仙台
• [Presentation] 感受性期に成立する匂い学習の神経生物学 (2013)
  • Author(s): 椛秀人
  • Organizer: 日本味と匂学会第47回大会日本味と匂学会賞受賞講演
  • Place of Presentation: 仙台市民会館,仙台
• [Presentation] フェロモン記憶と個体認知 (2013)
  • Author(s): 椛 秀人, 藤田博子, 吾妻 健, 奥谷文乃, 松波宏明
  • Organizer: 日本味と匂学会第47回大会
  • Place of Presentation: 仙台市民会館
  • Invited
• [Presentation] 感受性期に成立する匂い学習の神経生物学 (2013)
  • Author(s): 椛 秀人
  • Organizer: 日本味と匂学会第47回大会
  • Place of Presentation: 仙台市民会館
  • Invited
• [Presentation] A suppressing mechanism of the recurrent inhibi tion by group II metabotropic glut amate receptors activated by endog enous glutamate release from mitra l cells in the mouse accessory olf actory bulb (2012)
  • Author(s): Taniguchi M, Kaba H
  • Organizer: XVI International Sym posium on Olfaction and Taste
  • Place of Presentation: Stockholm Waterfron t, Stockholm, Sweden
• [Presentation] Mat ernally inherited peptides functio n as strain identity chemosignals in mice (2012)
  • Author(s): Kaba H, Fujita H, Matsunami H
  • Organizer: XVI International Symposi um on Olfaction and Taste
  • Place of Presentation: Stockholm Waterfron t, Stockholm, Sweden
• [Presentation] A functional rol e for protein synthesis in long-te rm potentiation at synapses in the mouse accessory olfactory bulb (2012)
  • Author(s): Murata Y, Kaba H
  • Organizer: X VI International Symposium on Olfa ction and Taste
  • Place of Presentation: Stockholm Waterfron t, Stockholm, Sweden
• [Presentation] Vasopressin serve s to promote the induction of syna ptic plasticity in the mouse acces sory olfactory bulb (2012)
  • Author(s): Namba T, Taniguchi M, Murata Y, Ok utani F, Kaba H
  • Organizer: XVI Internati onal Symposium on Olfaction and Ta ste
  • Place of Presentation: Stockholm Waterfron t, Stockholm, Sweden
• [Presentation] Maternally inherited peptides function as strain identity chemosignals in mice. (2012)
  • Author(s): Kaba H
  • Organizer: XVI International Symposium on Olfaction and Taste
  • Place of Presentation: Radisson Blu Waterfront hotel (Sweden)
• [Book] 脳神経科学イラストレイテッドー分子・細胞から実験技術まで (2013)
  • Author(s): 椛秀人
  • Publisher: 羊土社
• [Book] 脳神経科学イラストレイテッドー分子・細胞から実験技術まで (2013)
  • Author(s): 椛 秀人
  • Total Pages: 395
  • Publisher: 羊土社
• [Book] 解剖生理学〔第2版〕 (2012)
  • Author(s): 椛秀人
  • Publisher: 化学同人
• [Remarks]
  • URL: http://www.kochi-ms.ac.jp/~ff_phsl1/index.htm