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Single-cell recording of suprachiasmatic nucleus in rodent

Research Project

Project/Area Number 24659109
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

YAGITA Kazuhiro  京都府立医科大学, 医学(系)研究科(研究院), 教授 (90324920)

Co-Investigator(Kenkyū-buntansha) INOKAWA Hitoshi  京都府立医科大学, 医学研究科, 講師 (40285250)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords概日リズム / 視交叉上核 / 単一ニューロン / 電気活動 / 単一ニューロン記録
Research Abstract

Single-cell based neural activity recording from the suprachismatic nucleus is important to understand the resetting mechanism of circadian rhythm in mammals. We performed single-cell neural activity recording from living rat or mouse suprachiasmatic nucleus. We succeeded to record the single-cell neural activity responded to the light in mice and rats. It must be useful strategy to elucidate the resetting mechanisms in mammalian circadian clock.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (26 results)

All 2014 2013 2012 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (19 results) (of which Invited: 6 results)

  • [Journal Article] Cell and Tissue-autonomous development of the circadian clock in mouse embryos.2014

    • Author(s)
      Inada Y, Uchida H, Umemura Y, Nakamura W, Sakai T, Koike N, Yagita K.
    • Journal Title

      FEBS Lett

      Volume: 588 Issue: 3 Pages: 459-465

    • DOI

      10.1016/j.febslet.2013.12.007

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Prolonged Bioluminescence Monitoring in Mouse ex vivo Bone Culture Revealed Persistent Circadian Rhythms in Articular Cartilages and Growth Plates2013

    • Author(s)
      Okubo N, Minami Y, Fujiwara H, Umemura Y, Tsuchiya Y, Shirai Y, Oda R, Inokawa H, Kubo T, Yagita K
    • Journal Title

      PLoS One

      Volume: 8

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regional circadian period difference in the suprachiasmatic nucleus of the mammalian circadian center2013

    • Author(s)
      Koinuma S, Asakawa T, Nagano M, Furukawa K, Sujino M, Masumoto KH, Nakajima Y, Hashimoto S, Yagita K, Shigeyoshi Y
    • Journal Title

      Eur J Neurosci

      Volume: 38 Pages: 2832-41

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] An in vitro ES cell-based clock recapitulation assay model identifies CK2a as an endogenous clock regulator2013

    • Author(s)
      Umemura Y, Yoshida J, Wada M, Tsuchiya Y, Minami Y, Watanabe H, Kondoh G, Takeda J, Inokawa H, Horie K, Yagita K
    • Journal Title

      PLoS One

      Volume: 8

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] An in vitro ES cell-based clock recapitulation assay model identifies CK2a as an endogenous clock regulator2013

    • Author(s)
      Umemura Y, Yoshida J, Wada M, Tsuchiya Y, Minami Y, Watanabe H, Kondoh G, Takeda J, Inokawa H, Horie K, Yagita K.
    • Journal Title

      PLoS One

      Volume: 8 Issue: 6 Pages: e67241-e67241

    • DOI

      10.1371/journal.pone.0067241

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regional circadian period difference in the suprachiasmatic nucleus of the mammalian circadian center.2013

    • Author(s)
      Koinuma S, Asakawa T, Nagano M, Furukawa K, Sujino M, Masumoto KH, Nakajima Y, Hashimoto S, Yagita K, Shigeyoshi Y.
    • Journal Title

      Eur J Neurosci.

      Volume: 38 Issue: 6 Pages: 2832-41

    • DOI

      10.1111/ejn.12308

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mammalian TIMELESS is involved in period determination and DNA damage-dependent phase advancing of the circadian clock.2013

    • Author(s)
      Engelen E, Janssens RC, Yagita K, Smits VA, van der Horst GT, Tamanini F
    • Journal Title

      PLoS One

      Volume: 8 Issue: 2 Pages: e56623-e56623

    • DOI

      10.1371/journal.pone.0056623

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Misregulation of transcriptional program controlling the cellular differentiation resulted in disruption of the circadian clock development2014

    • Author(s)
      八木田和弘(シンポジスト・オーガナイザー)
    • Organizer
      日本生理学会大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2014-03-16
    • Related Report
      2013 Final Research Report
  • [Presentation] MYC induced disruption of circadian clock development2013

    • Author(s)
      八木田和弘(シンポジスト・オーガナイザー)
    • Organizer
      第86回日本生化学会
    • Place of Presentation
      横浜
    • Year and Date
      2013-12-11
    • Related Report
      2013 Final Research Report
  • [Presentation] イメージング技術を用いた生体機能のCutting Edge2013

    • Author(s)
      八木田和弘
    • Organizer
      関西実験動物研究会30周年記念大会
    • Place of Presentation
      京都(シンポジスト)
    • Year and Date
      2013-12-06
    • Related Report
      2013 Final Research Report
  • [Presentation] ES細胞を用いた概日時計のin vitro再現と分化制御機構との関連2013

    • Author(s)
      八木田和弘(オーガナイザー)
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸(ワークショップ講演)
    • Year and Date
      2013-12-05
    • Related Report
      2013 Final Research Report
  • [Presentation] 体内時計はいつ動き出すか2013

    • Author(s)
      八木田和弘(シンポジスト・オーガナイザー)
    • Organizer
      第14回日本抗加齢医学会
    • Place of Presentation
      横浜
    • Year and Date
      2013-06-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Circadian Clock : A cell-autonomous time-keeping system in our body2013

    • Author(s)
      Yagita K
    • Organizer
      6^<th> Asia Oceania Conference of Photobiology
    • Place of Presentation
      Sydney(Invited speaker)
    • Related Report
      2013 Final Research Report
  • [Presentation] ES cell-based in vitro evaluation system of circadian clock phenotypes in mammals2013

    • Author(s)
      八木田和弘(シンポジスト・オーガナイザー)
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] ES cell-based approach elucidating the integrated physiology of mammalian circadian systems2012

    • Author(s)
      Yagita K
    • Organizer
      4th International Symposium on Photic Bioimaging 2012
    • Place of Presentation
      Sapporo(Invited speaker)
    • Related Report
      2013 Final Research Report
  • [Presentation] Cell-autonomous development of mammalian circadian clock during the differentiation culture of ES cells2012

    • Author(s)
      Yagita K
    • Organizer
      International Conference for Histochemistry and Cytochemistry 2012
    • Place of Presentation
      Kyoto(Invited speaker)
    • Related Report
      2013 Final Research Report
  • [Presentation] 体内時計の発生・発達2012

    • Author(s)
      八木田和弘
    • Organizer
      第1回日本発達神経科学会
    • Place of Presentation
      明石(特別講演)
    • Related Report
      2013 Final Research Report
  • [Presentation] 発生発達期における概日リズムの階層的形成2012

    • Author(s)
      八木田和弘
    • Organizer
      第17回日本行動神経内分泌研究会
    • Place of Presentation
      京都(教育講演)
    • Related Report
      2013 Final Research Report
  • [Presentation] 時間軸生物学 : 細胞分化と概日時計の接点2012

    • Author(s)
      八木田和弘(シンポジスト・オーガナイザー)
    • Organizer
      第12回日本抗加齢医学会総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] ES cell-based evaluation system of circadian phenotypes in mammals.2012

    • Author(s)
      Kazuhiro Yagita
    • Organizer
      13th Society for Research on Biological Rhythms
    • Place of Presentation
      Destin, Florida
    • Related Report
      2012 Research-status Report
  • [Presentation] Circadian Clock: A cell-autonomous time-keeping system in our body.

    • Author(s)
      Kazuhiro Yagita
    • Organizer
      6th Asia Oceania Conference of Photobiology,
    • Place of Presentation
      Sydney
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Misregulation of transcriptional program controlling the cellular differentiation resulted in disruption of the circadian clock development.

    • Author(s)
      八木田和弘
    • Organizer
      第91回日本生理学会大会
    • Place of Presentation
      鹿児島
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ES cell-based approach elucidating the integrated physiology of mammalian circadian systems.

    • Author(s)
      Kazuhiro Yagita
    • Organizer
      4th International Symposium on Photic Bioimaging 2012
    • Place of Presentation
      Sapporo
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Cell-autonomous development of mammalian circadian clock during the differentiation culture of ES cells.

    • Author(s)
      Kazuhiro Yagita
    • Organizer
      International Conference for Histochemistry and Cytochemistry 2012
    • Place of Presentation
      Kyoto
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 体内時計の発生・発達

    • Author(s)
      八木田和弘
    • Organizer
      第1回日本発達神経科学会
    • Place of Presentation
      明石
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 時間軸生物学:細胞分化と概日時計の接点

    • Author(s)
      八木田和弘
    • Organizer
      第12回日本抗加齢医学会総会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
    • Invited

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Published: 2013-05-31   Modified: 2019-07-29  

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