S-nitrosylation sensitive fluorescent protein
| Project/Area Number |
24659111
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General pharmacology
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| Research Institution | Chiba University |
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Principal Investigator |
MATSUMOTO Akio 千葉大学, 医学(系)研究科(研究院), 准教授 (60437308)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | Ｓ－ニトロシル化 / 一酸化窒素 / 翻訳後修飾 / イメージング / GFP / S-nitrosylation / Ｓーニトロシル化 |
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| Research Abstract |
The major focus of this project is to provide a method to analyze the formation of S-nitrosothiol (SNO) on cysteine residue, which is necessary to study the physiological function of nitric oxide in the cell. We worked on green fluorescent protein to use it as a reporter system to sense the SNO-formation by introducing a cysteine followed by random mutations in nucleotides sequence of GFP. Some clones showed fluorescent intensity changes following an SNO-treatment, and the sequence analysis indicates that the structure modification has greater influence on SNO-formation than other sequence changes.
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Report
(3 results)
Research Products
(37 results)
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[Journal Article] Oral 'hydrogen water' induces neuroprotective ghrelin secretion in mice.2013
Author(s)
Matsumoto, A., Yamafuji, M., Tachibana, T., Nakabeppu, Y., Noda, M., and Nakaya, H.
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Journal Title
Sci Rep
Volume: 3
Issue: 1
Pages: 3273-3273
DOI
Related Report
Peer Reviewed
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[Journal Article] N-acetylglucosaminyltransferase (GnT) assays using fluorescent oligosaccharide acceptor substrates: GnT-III, IV, V, and IX (GnT-Vb).2013
Author(s)
Takamatsu S, Korekane H, Ohtsubo K, Oguri S, Park JY, Matsumoto A, Taniguchi N.
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Journal Title
Methods Mol Biol.
Volume: 1022
Pages: 283-298
DOI
ISBN
9781627034647, 9781627034654
Related Report
Peer Reviewed
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[Journal Article] Identification of Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 (ENPP3) as a Regulator of N-Acetylglucosaminyltransferase GnT-IX (GnT-Vb)2013
Author(s)
Korekane H, Park JY, Matsumoto A, Nakajima K, Takamatsu S, Ohtsubo K, Miyamoto Y, Hanashima S, Kanekiyo K, Kitazume S, Yamaguchi Y, Matsuo I, Taniguchi N
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Journal Title
J. Biol. Chem.
Volume: 288(39)
Issue: 39
Pages: 27912-27926
DOI
Related Report
Peer Reviewed
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[Journal Article] Sensitivity of Heterozygous α1,6-Fucosyltransferase Knock-out Mice to Cigarette Smoke-induced Emphysema: IMPLICATION OF ABERRANT TRANSFORMING GROWTH FACTOR-β; SIGNALING AND MATRIX METALLOPROTEINASE GENE EXPRESSION.2012
Author(s)
Gao C, Maeno T, Ota F, Ueno M, Korekane H, Takamatsu S, Shirato K, Matsumoto A, Kobayashi S, Yoshida K, Kitazume S, Ohtsubo K, Betsuyaku T, Taniguchi N
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Journal Title
J. Biol. Chem.
Volume: 287(20)
Issue: 20
Pages: 16699-16708
DOI
Related Report
Peer Reviewed
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