S-nitrosylation sensitive fluorescent protein
| Project/Area Number |
24659111
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General pharmacology
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| Research Institution | Chiba University |
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Principal Investigator |
MATSUMOTO Akio 千葉大学, 医学(系)研究科(研究院), 准教授 (60437308)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | Ｓ－ニトロシル化 / 一酸化窒素 / 翻訳後修飾 / イメージング / GFP / S-nitrosylation / Ｓーニトロシル化 |
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| Research Abstract |
The major focus of this project is to provide a method to analyze the formation of S-nitrosothiol (SNO) on cysteine residue, which is necessary to study the physiological function of nitric oxide in the cell. We worked on green fluorescent protein to use it as a reporter system to sense the SNO-formation by introducing a cysteine followed by random mutations in nucleotides sequence of GFP. Some clones showed fluorescent intensity changes following an SNO-treatment, and the sequence analysis indicates that the structure modification has greater influence on SNO-formation than other sequence changes.
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Report
(3 results)
Research Products
(37 results)
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[Journal Article] Identification of ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3) as a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb).2014
Author(s)
Korekane H, Park JY, Matsumoto A, Nakajima K, Takamatsu S, Ohtsubo K, Miyamoto Y, Hanashima S, Kanekiyo K, Kitazume S, Yamaguchi Y, Matsuo I, Taniguchi N
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Journal Title
J Biol Chem.
Volume: 288(39)
Pages: 27912-27926
DOI
Related Report
Peer Reviewed
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[Journal Article] Oral"hydrogen water"induces neuroprotective ghrelin secretion in mice2013
Author(s)
Matsumoto, A., Yamafuji, M., Tachibana, T., Nakabeppu, Y., Noda, M., and Nakaya, H.
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Journal Title
Scientific reports
Volume: 3
Pages: 3273-3273
DOI
Related Report
Peer Reviewed
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[Journal Article] Sensitivity of heterozygous α1, 6 fucosyltransferase knock out mice to cigarette smoke-induced emphysema: implication of aberrant TGF-β signaling and MMP gene expression2012
Author(s)
Gao C, Maeno T, Ota F, Ueno M, Korekane H, Takamatsu S, Shirato K, Matsumoto A, Kobayashi S, Yoshida K, Kitazume S, Ohtsubo K, Betsuyaku T, Taniguchi N
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Journal Title
Journal of Biological Chemistry
Volume: 287(20)
Pages: 16699-16708
DOI
Related Report
Peer Reviewed
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