Endothelial microparticles modulate cellular status of pericytes

• Project/Area Number: 24659112
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: General pharmacology
• Research Institution: University of Toyama
• Principal Investigator: YAMAMOTO Seiji 富山大学, 医学薬学研究部(医学), 助教 (10456361)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: EMP / Endothelial cells / Pericytes / 血管内皮細胞 / 炎症

Research Abstract

We studied endothelial microparticles (EMPs) produced from inflamed endothelial cells in vitro. EMPs incorporation study was performed, and mRNA and protein expression levels were analyzed using pericytes by the action of the EMPs. Here we show that EMPs contained inflammatory specific miRNA. EMPs incorporated pericytes were altered their transcription and protein expression levels. We have shown that target cells of EMPs are pericytes, suggesting EMPs and miRNA included in the EMPs are new therapeutic target of the various inflammatory diseases.

Research Products

• [Journal Article] PDGFR-βas a positive regulator of tissue repair in a mouse model of focal cerebral ischemia (2012)
  • Author(s): Shen J, IshiiY, Xu G, Dang TC, Hamashima T, Matsushima T, Yamamoto S, Hattori Y, Takatsuru Y, Nabekura J, Sasahara M
  • Journal Title: J Cereb Blood Flow Metabol Volume: 32 Pages: 353-367
  • Peer Reviewed
• [Journal Article] Identification of innate IL-5-producing cells and their role in lung eosinophil regulation and antitumor immunity (2012)
  • Author(s): Ikutani M, Yanagibashi T, Ogasawara M, Tsuneyama K, Yamamoto S, Hattori Y, Kouro T, Itakura A, Nagai Y, Takaki S, Takatsu K
  • Journal Title: J Immunol Volume: 188 Pages: 703-713
  • Peer Reviewed
• [Journal Article] Role of ion channels in sepsis-induced atrial tachyarrhythmias in guinea pigs (2012)
  • Author(s): Aoki Y, Hatakeyama N, Yamamoto S, Kinoshita H, Matsuda N, Hattori Y, Yamazaki M
  • Journal Title: Br J Pharmacol Volume: 166 Pages: 390-400
  • Peer Reviewed
• [Journal Article] PDGFR-βas a positive regulator of tissue repair in a mouse model of focal cerebral ischemia (2012)
  • Author(s): Shen J.
  • Journal Title: J Cereb.Blood Flow Metab. Volume: 32 Issue: 2 Pages: 353-367
  • DOI: 10.1038/jcbfm.2011.136
  • Peer Reviewed
• [Presentation] Pericyte in Health and Disease (2014)
  • Author(s): 山本誠士
  • Organizer: 脈管クラスター研究講演会
  • Place of Presentation: 旭川
• [Presentation] マウス創傷治癒モデルにおける血管新生および恒常性に関与するmultifunctional macrophageの機能解析 (2013)
  • Author(s): 東英梨月,山本誠士,村松昌,濱島丈,石井陽子,新飯田俊平,笹原正清
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸(P2-0553)
  • Year and Date: 2013-12-04
• [Presentation] 血管内皮細胞が産生するEndothelial microparticlesの病態生物学的解析 (2013)
  • Author(s): 山本誠士,東英梨月,村松昌,濱島丈,石井陽子,新飯田俊平,笹原正清
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸(P1-0928)
  • Year and Date: 2013-12-03
• [Presentation] 血管内皮炎症によって産生されるEMPの機能解析 (2013)
  • Author(s): 山本誠士,東英梨月,村松昌,濱島丈,石井陽子,新飯田俊平,笹原正清
  • Organizer: 第21回日本血管生物医学会
  • Place of Presentation: 大阪(PB1-22)
  • Year and Date: 2013-09-26
• [Presentation] 血管内皮細胞の炎症病態に関連するmiRNAの解析 (2013)
  • Author(s): 山本誠士,東英梨月,村松昌,濱島丈,石井陽子,新飯田俊平,笹原正清
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: 札幌(2-G4-5)
  • Year and Date: 2013-06-07
• [Presentation] 血管内皮細胞が産生するEndothelial microparticlesの病態生物学的解析 (2013)
  • Author(s): 4. 山本誠士, 東英梨月, 村松昌, 濱島丈, 石井陽子, 新飯田俊平, 笹原正清
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] マウス創傷治癒モデルにおける血管新生および恒常性に関与するmultifunctional macrophageの機能解析 (2013)
  • Author(s): 5. 東英梨月, 山本誠士, 村松昌, 濱島丈, 石井陽子, 新飯田俊平, 笹原正清
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] 血管内皮炎症によって産生されるEMPの機能解析 (2013)
  • Author(s): 7. 山本誠士, 東英梨月, 村松昌, 濱島丈, 石井陽子, 新飯田俊平, 笹原正清
  • Organizer: 第21回日本血管生物医学会
  • Place of Presentation: 大阪
• [Presentation] 血管内皮細胞の炎症病態に関連するmiRNAの解析 (2013)
  • Author(s): 10. 山本誠士, 東英梨月, 村松昌, 濱島丈, 石井陽子, 新飯田俊平, 笹原正清
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: 札幌
• [Presentation] Endothelial microparticles (EMPs)由来miRNAの機能解析 (2012)
  • Author(s): 山本誠士,東英梨月,村松昌,柳橋努,生谷尚士,長井良憲,高津聖志,堂本光子,松田直之,新飯田俊平,服部裕一
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡(2P-0355)
  • Year and Date: 2012-12-12
• [Presentation] 骨髄由来multifunctional macrophageのマウス創傷治癒モデルにおける機能解析 (2012)
  • Author(s): 東英梨月,山本誠士,村松昌,新飯田俊平,服部裕一
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡(1P-0479)
  • Year and Date: 2012-12-11
• [Presentation] Functional analysis of miRNA contained in the endothelial microparticles (EMPs) (2012)
  • Author(s): Yamamoto S, Azuma E, Muramatsu M, Yanagibashi T, Ikutani M, Nagai Y, Takatsu K, Dohmoto M, Matsuda N, Niida S, Hattori Y
  • Organizer: The 20th Annual Meeting of the Japanese Vascular Biology and Medicine Organization
  • Place of Presentation: Tokushima(O-37)
  • Year and Date: 2012-12-07
• [Presentation] Technological developments and analyses of in vitro neuro-vascular wiring systems (2012)
  • Author(s): Yamamoto S, Sasahara M
  • Organizer: Neuro-Vascular Wiring International Symposium 2012
  • Place of Presentation: Nara, Japan(31)
  • Year and Date: 2012-11-12
• [Presentation] 血中miRNAとEndothelial Microparticles (2012)
  • Author(s): 新飯田俊平,山本誠士,村松昌,東英梨月,本山昇,滝川修
  • Organizer: 第4回RNAi研究会
  • Place of Presentation: 広島
  • Year and Date: 2012-09-01
• [Presentation] Inflammatory endothelial microparticles (EMPs) contribute to cellular interaction as a bioactive carrier (2012)
  • Author(s): Yamamoto S, Azuma E, Yanagibashi T, Ikutani M, Nagai Y, Takatsu K, Miyazaki K, Dohmoto M, Muramatsu M, Matsuda N, Niida S, Hattori Y
  • Organizer: The 17th International Vascular Biology Meeting
  • Place of Presentation: Wiesbaden, Germany(PII-34)
  • Year and Date: 2012-06-03
• [Presentation] Technological developments and analyses of in vitro neuro-vascular wiring systems (2012)
  • Author(s): Yamamoto S
  • Organizer: Neuro-Vascular Wiring International Symposium 2012
  • Place of Presentation: Nara, Japan
• [Presentation] Inflammatory endothelial microparticles (EMPs) contribute to cellular interaction as a bioactive carrier (2012)
  • Author(s): Yamamoto S
  • Organizer: The 17th International Vascular Biology Meeting
  • Place of Presentation: Wiesbaden, Germany
• [Presentation] Endothelial microparticles (EMPs)由来miRNAの機能解析 (2012)
  • Author(s): 山本誠士
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡, 日本
• [Presentation] 骨髄由来multifunctional macrophageのマウス創傷治癒モデルにおける機能解析 (2012)
  • Author(s): 東英梨月
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡, 日本
• [Presentation] Functional analysis of miRNA contained in the endothelial microparticles (EMPs) (2012)
  • Author(s): Yamamoto S
  • Organizer: The 20th Annual Meeting of the Japanese Vascular Biology and Medicine Organization
  • Place of Presentation: 徳島, 日本
• [Remarks]
  • URL: http://www.med.u-toyama.ac.jp/pathol2/index.html
• [Remarks]
  • URL: http://utomir.lib.u-toyama.ac.jp/dspace/handle/10110/11902
• [Remarks] 富山大学 病態病理学
  • URL: http://www.med.u-toyama.ac.jp/pathol2/index.html
• [Remarks] 富山大学学術情報リポジトリ
  • URL: http://utomir.lib.u-toyama.ac.jp/dspace/handle/10110/11902