Epigenetic regulation involved in osteoclast differentiation

• Project/Area Number: 24659115
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: General pharmacology
• Research Institution: Osaka University
• Principal Investigator: NISHIKAWA Keizo 大阪大学, 免疫学フロンティア研究センター, 特任助教(常勤) (30516290)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 破骨細胞 / エピジェネティクス / 転写因子 / 転写制御 / 細胞内代謝 / メタボライト / SAM / DNAメチル化

Research Abstract

Although transcriptional regulation involved in osteoclast differentiation has extensively studied, epigenetics in osteoclasts have poorly understood. Our studies have demonstrated that methyltransferase enzyme whose expression is increased during osteoclastogenesis play an important role in osteoclast differentiation.

Research Products

• [Journal Article] 遺伝子操作を用いた新規レポーターマウスの作出方法 (2013)
  • Author(s): 西川恵三
  • Journal Title: 実験医学別冊 Pages: 202-211
• [Journal Article] Cell cycle-dependent Rho GTPase activity dynamically regulates cancer cell motility and invasion in vivo (2013)
  • Author(s): Kagawa Y, Matsumoto S, Kamioka Y, Mimori K, Naito Y, Ishii T, Okuzaki D, Nishida N, Maeda S, Naito A, Kikuta J, Nishikawa K, Nishimura J, Haraguchi N, Takemasa I, Mizushima T, Ikeda M, Yamamoto H, Sekimoto M, Ishii H, Doki Y, Matsuda M, Kikuchi A, Mori M, Ishii M
  • Journal Title: PLoS one Volume: 30
• [Journal Article] Development of an in vitro culture method for stepwise differentiation of mouse embryonic stem cells and induced pluripotent stem cell into mature osteoclasts. (2013)
  • Author(s): Nishikawa K.et. al.
  • Journal Title: Journal of Bone and Mineral Metabolism Volume: 未定
  • Peer Reviewed
• [Journal Article] Cell cycle-dependent Rho GTPase activity dynamically regulates cancer cell motility and invasion in vivo. (2013)
  • Author(s): Kagawa Y.et. al.
  • Journal Title: PLoS One Volume: 30
  • Peer Reviewed
• [Journal Article] 老人性骨粗鬆症に関る分子機構の解明と創薬応用 (2012)
  • Author(s): 西川恵三
  • Journal Title: 日本老年医学会雑誌 Volume: 49 Pages: 314-317
• [Journal Article] Development of an in vitro culture method for stepwise differentiation of mouse embryonic stem cells and induced pluripotent stem cell into mature osteoclasts
  • Author(s): Nishikawa K, Iwamoto Y, Ishii M
  • Journal Title: Journal of Bone and Mineral Metabolism Volume: (In press)
• [Presentation] Development of an in vitro culture method for stepwise differentiation of mouse embryonic stem cells and induced pluripotent stem cell into mature osteoclasts (2014)
  • Author(s): 西川恵三、岩本依子、石井優
  • Organizer: 第87回日本薬理学会年会
  • Place of Presentation: 宮城
  • Year and Date: 2014-03-20
• [Presentation] Development of an in vitro culture method for stepwise differentiation of mouse embryonic stem cells and induced pluripotent stem cell into mature osteoclasts. (2014)
  • Author(s): 西川恵三、岩本依子、石井優
  • Organizer: 第87回日本薬理学会年会
  • Place of Presentation: 仙台国際センター(宮城)
• [Presentation] Dnmt3a as a novel nexus coordinating metabolism and epigenetics in osteoclasts (2013)
  • Author(s): 西川恵三
  • Organizer: 13th IFReC Colloquium
  • Place of Presentation: 大阪
  • Year and Date: 2013-12-18
• [Presentation] 抑制性転写制御から理解する破骨細胞分化の制御機構 (2013)
  • Author(s): 西川恵三
  • Organizer: 第4回新医学領域創生セミナー
  • Place of Presentation: 宮城
  • Year and Date: 2013-05-23
• [Presentation] 破骨細胞分化にかかわる細胞内エネルギー代謝調節の分子機構と生理的意義の理解 (2013)
  • Author(s): 西川恵三
  • Organizer: 若手ワークショップ@鬼怒川
  • Place of Presentation: 栃木
  • Year and Date: 2013-01-25
• [Presentation] 抑制性転写制御から理解する破骨細胞分化の制御機構 (2013)
  • Author(s): 西川恵三
  • Organizer: 第４回新医学領域創生セミナー
  • Place of Presentation: 東北大学(宮城)
  • Invited
• [Presentation] Dnmt3a as a novel nexus coordinating metabolism and epigenetics in osteoclasts (2013)
  • Author(s): 西川恵三
  • Organizer: 13th IFReC Colloquium
  • Place of Presentation: 大阪大学(大阪)
  • Invited
• [Presentation] 胚性幹細胞・誘導多能性幹細胞から破骨細胞を分化誘導する培養法の開発 (2013)
  • Author(s): 西川恵三、岩本依子、石井優
  • Organizer: 第86回日本生化学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
• [Presentation] 破骨細胞分化にかかわる細胞内エネルギー代謝調節の分子機構と生理的意義の理解 (2013)
  • Author(s): 西川恵三
  • Organizer: 若手ワークショップ＠鬼怒川
  • Place of Presentation: ホテル鬼怒川御苑(栃木)
• [Presentation] 胚性幹細胞・誘導多能性幹細胞から破骨細胞を分化誘導する培養法の開発 (2012)
  • Author(s): 西川恵三、岩本依子、石井優
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-12
• [Presentation] 骨生物学の分子基盤の解明と創薬応用 (2012)
  • Author(s): 西川恵三
  • Organizer: 奈良先端未来開拓コロキウム
  • Place of Presentation: 奈良
  • Year and Date: 2012-12-07
• [Presentation] 破骨細胞のエピジェネティック制御機構の解明 (2012)
  • Author(s): 西川恵三
  • Organizer: アステラス病態代謝研究報告会
  • Place of Presentation: 東京
  • Year and Date: 2012-10-20
• [Presentation] 胚性幹細胞・誘導多能性幹細胞から破骨細胞を分化誘導する培養法の開発 (2012)
  • Author(s): 西川恵三、岩本依子、石井優
  • Organizer: 第第86回日本生化学会年会
  • Place of Presentation: 神奈川
  • Year and Date: 2012-09-12
• [Presentation] 破骨細胞のエピジェネティック制御機構の解明 (2012)
  • Author(s): 西川恵三
  • Organizer: アステラス病態代謝研究報告会
  • Place of Presentation: 経団連会館(東京)
  • Invited
• [Presentation] 骨生物学の分子基盤の解明と創薬応用 (2012)
  • Author(s): 西川恵三
  • Organizer: 奈良先端未来開拓コロキウム
  • Place of Presentation: 奈良先端大学(奈良)
  • Invited
• [Presentation] 胚性幹細胞・誘導多能性幹細胞から破骨細胞を分化誘導する培養法の開発 (2012)
  • Author(s): 西川恵三
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡(福岡)