Identification of plasma membrane leucine receptor and elucidation of its link to mTOR signaling pathway
| Project/Area Number |
24659116
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General pharmacology
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| Research Institution | Osaka University |
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Principal Investigator |
KANAI Yoshikatsu 大阪大学, 医学(系)研究科(研究院), 教授 (60204533)
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| Co-Investigator(Kenkyū-buntansha) |
NAGAMORI Shushi 大阪大学, 医学系研究科, 准教授 (90467572)
OHGAKI Ryuichi 大阪大学, 医学系研究科, 助教 (20467525)
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| Co-Investigator(Renkei-kenkyūsha) |
HARI Yoshiyuki 大阪大学, 薬学研究科, 特任准教授 (50347423)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 受容体 / チャネル / 輸送系 / シグナル情報伝達系 / 比較定量プロテオミクス / リン酸化プロテオミクス / 生体膜 / プロテオミクス |
|---|
| Research Abstract |
By comparative quantitative proteomics using mass spectrometry, several candidates for the plasma membrane leucine receptor were identified. Functional analysis of these candidates revealed that one protein plays a role in mTORC1 pathway downstream of growth factor receptors and thereby regulates the activity of mTORC1 cooperatively with amino acids. We also developed a new analytical procedure of mass spectrometry suitable for integral membrane proteins by improving the sample preparation process and mass spectrometric measurement, because the detection and identification of integral membrane proteins were difficult in general. Additionally, to reveal the overall signaling events downstream of leucine receptor, we established a method for quantitative phosphoproteomics. The technical basis fundamentally important for the identification of leucine receptor has been established in this study.
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Report
(3 results)
Research Products
(80 results)
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[Journal Article] Down-regulation of placental mTOR, insulin/IGF-I signaling, and nutrient transporters in response to maternal nutrient restriction in the baboon2014
Author(s)
Kavitha JV, Rosario FJ, Nijland MJ, McDonald TJ, Wu G, Kanai Y, Powell TL, Nathanielsz PW, Jansson T
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Journal Title
FASEB J
Volume: 28
Issue: 3
Pages: 1294-1305
DOI
Related Report
Peer Reviewed
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[Journal Article] JPH203, an L-Type Amino Acid Transporter 1–Selective Compound, Induces Apoptosis of YD-38 Human Oral Cancer Cells2014
Author(s)
Yun DW, Lee SA, Park MG, Kim JS, Yu SK, Park MR, Kim SG, Oh JS, Kim CS, Kim HJ, Kim JS, Chun HS, Kanai Y, Endou H, Wempe MF, Kim do K.
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Journal Title
Journal of Pharmacological Sciences
Volume: 124
Issue: 2
Pages: 208-217
DOI
NAID
ISSN
1347-8613, 1347-8648
Related Report
Peer Reviewed
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[Journal Article] Clinical significance of L-type amino acid transporter 1 expression as a prognostic marker and potential of new targeting therapy in biliary tract cancer2013
Author(s)
Kaira K, Sunose Y, Ohshima Y, Ishioka NS, Arakawa K, Ogawa T, Sunaga N, Shimizu K, Tominaga H, Oriuchi N, Itoh H, Nagamori S, Kanai Y, Yamaguchi A, Segawa A, Ide M, Mori M, Oyama T, Takeyoshi I
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Journal Title
BMC Cancer
Volume: 16
Issue: 1
Pages: 482-482
DOI
Related Report
Peer Reviewed
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[Journal Article] Diagnostic usefulness of F-FAMT PET and L-type amino acid transporter 1 (LAT1) expression in oral squamous cell carcinoma.2013
Author(s)
Nobusawa A, Kim M, Kaira K, Miyashita G, Negishi A, Oriuchi N, Higuchi T, Tsushima Y, Kanai Y, Yokoo S, Oyama T.
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Journal Title
Eur J Nucl Med Mol
Volume: 40
Issue: 11
Pages: 1692-1700
DOI
Related Report
Peer Reviewed
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[Journal Article] IUPHAR-DB: updated database content and new features.2013
Author(s)
Sharman JL, Benson HE, Pawson AJ, Lukito v, Mpamhanga CP, Bombail v, Davenport AP, Peters JA, Spedding M, Harmar AJ, NC-IUPHAR; Alexander SP, Bonner TI, Catterall WA, Christopoulos A, Davenport AP, Dollery CT, Enna S, Harmar AJ, Kaibuchi K, Kanai Y, Laudet v, Neubig RR, Ohlstein EH, Peters JA, Pin JP, Ruegg U, Spedding M, Wright M W, du Souich P.
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Journal Title
Nucleic Acids Res
Volume: 41
Issue: D1
Pages: D1083-D1088
DOI
Related Report
Peer Reviewed
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[Journal Article] Transport of 3-fluoro-L-α-methyl-tyrosine by tumor-upregulated L-type amino acid transporter 1: a cause of the tumor uptake in PET.2012
Author(s)
Wiriyasermkul P, Nagamori S, Tominaga H, Oriuchi N, Kaira K, Nakao H, Kitashoji T, Ohgaki R, Tanaka H, Endou H, Endo K, Sakurai H, Kanai Y.
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Journal Title
J Nucl Med.
Volume: 53
Issue: 8
Pages: 1253-1261
DOI
Related Report
Peer Reviewed
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[Journal Article] How to use the IUPHAR receptor database to navigate pharmacological data.2012
Author(s)
Mpamhanga CP, S harm an JL, Harmar AJ; NC-IUPHAR; Alexander SP, Bonner TI, Catterall WA, Christopoulos A, Davenport AP, Dollery CT, Enna S, Harmar AJ, Kaibuchi K, Kanai Y, Laudet V, Neubig RR, Oh I stein EH, Peters J, Pin JP, Ruegg U, du Souich P, Spedding M, Wright MW.
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Journal Title
Methods Mol Biol.
Volume: 897
Pages: 15-29
DOI
ISBN
9781617799082, 9781617799099
Related Report
Peer Reviewed
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[Presentation] Transport of 4-borono-L-phenylalanine, a 10B carrier of boron neutron capture therapy, by system L amino acid transporters.
Author(s)
Printip Wongthai, Kohei Hagiwara, Yurika Miyoshi, Pattama Wiriyasermkul, Ryuichi Ohgaki, Kenjiro Tadagaki, Kenji Hamase, Shushi Nagamori, Yoshikatsu Kanai
Organizer
第124回日本薬理学会近畿部会
Place of Presentation
京都
Related Report
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[Presentation] Mechanisms of tumor cell uptake of 4-borono-phenylalanine, a 10B carrier of boron neutron capture therapy, mediated by system L amino acid transporters.
Author(s)
Printip Wongthai, Kohei Hagiwara, Yurika Miyoshi, Pattama Wiriyasermkul, Pornparn Kongpracha, Isozumi Noriyoshi, Ryuichi Ohgaki, Kenjiro Tadagaki, Kenji Hamase, Shushi Nagamori, Yoshikatsu Kanai
Organizer
第87回日本薬理学会年会
Place of Presentation
仙台
Related Report
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