Identification of plasma membrane leucine receptor and elucidation of its link to mTOR signaling pathway

• Project/Area Number: 24659116
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: General pharmacology
• Research Institution: Osaka University
• Principal Investigator: KANAI Yoshikatsu 大阪大学, 医学(系)研究科(研究院), 教授 (60204533)
• Co-Investigator(Kenkyū-buntansha): NAGAMORI Shushi 大阪大学, 医学系研究科, 准教授 (90467572) ; OHGAKI Ryuichi 大阪大学, 医学系研究科, 助教 (20467525)
• Co-Investigator(Renkei-kenkyūsha): HARI Yoshiyuki 大阪大学, 薬学研究科, 特任准教授 (50347423)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 受容体 / チャネル / 輸送系 / シグナル情報伝達系 / 比較定量プロテオミクス / リン酸化プロテオミクス / 生体膜 / プロテオミクス

Research Abstract

By comparative quantitative proteomics using mass spectrometry, several candidates for the plasma membrane leucine receptor were identified. Functional analysis of these candidates revealed that one protein plays a role in mTORC1 pathway downstream of growth factor receptors and thereby regulates the activity of mTORC1 cooperatively with amino acids. We also developed a new analytical procedure of mass spectrometry suitable for integral membrane proteins by improving the sample preparation process and mass spectrometric measurement, because the detection and identification of integral membrane proteins were difficult in general. Additionally, to reveal the overall signaling events downstream of leucine receptor, we established a method for quantitative phosphoproteomics. The technical basis fundamentally important for the identification of leucine receptor has been established in this study.

Research Products

• [Journal Article] Down-regulation of placental mTOR, insulin/IGF-I signaling, and nutrient transporters in response to maternal nutrient restriction in the baboon (2014)
  • Author(s): Kavitha JV, Rosario FJ, Nijland MJ, McDonald TJ, Wu G, Kanai Y, Powell TL, Nathanielsz PW, Jansson T
  • Journal Title: FASEB J Volume: 28 Issue: 3 Pages: 1294-1305
  • DOI: 10.1096/fj.13-242271
  • Peer Reviewed
• [Journal Article] JPH203, an L-Type Amino Acid Transporter 1–Selective Compound, Induces Apoptosis of YD-38 Human Oral Cancer Cells (2014)
  • Author(s): Yun DW, Lee SA, Park MG, Kim JS, Yu SK, Park MR, Kim SG, Oh JS, Kim CS, Kim HJ, Kim JS, Chun HS, Kanai Y, Endou H, Wempe MF, Kim do K.
  • Journal Title: Journal of Pharmacological Sciences Volume: 124 Issue: 2 Pages: 208-217
  • DOI: 10.1254/jphs.13154FP
  • NAID: 130003391478
  • ISSN: 1347-8613, 1347-8648
  • Peer Reviewed
• [Journal Article] Clinical significance of L-type amino acid transporter 1 expression as a prognostic marker and potential of new targeting therapy in biliary tract cancer (2013)
  • Author(s): Kaira K, Sunose Y, Ohshima Y, Ishioka NS, Arakawa K, Ogawa T, Sunaga N, Shimizu K, Tominaga H, Oriuchi N, Itoh H, Nagamori S, Kanai Y, Yamaguchi A, Segawa A, Ide M, Mori M, Oyama T, Takeyoshi I
  • Journal Title: BMC Cancer Volume: 16 Issue: 1 Pages: 482-482
  • DOI: 10.1186/1471-2407-13-482
  • Peer Reviewed
• [Journal Article] The SLC3 and SLC7 families of amino acid transporters (2013)
  • Author(s): Fotiadis D, Kanai Y, Palacin M
  • Journal Title: Mol Aspects Med Volume: 34 Issue: 2-3 Pages: 139-158
  • DOI: 10.1016/j.mam.2012.10.007
  • Peer Reviewed
• [Journal Article] Palacin M. The small SLC43 family: facilitator system L amino acid transporters and the orphan EEG1. (2013)
  • Author(s): Bodoy S, Fotiadis D, Stoeger C, Kanai Y, Palacin M.
  • Journal Title: Mol Aspects Med Volume: Apr;34(2-3)(Epub 2012 Dec 23) Issue: 2-3 Pages: 638-645
  • DOI: 10.1016/j.mam.2012.12.006
  • Peer Reviewed
• [Journal Article] The SLC3 and SLC7 families of amino acid transporters. (2013)
  • Author(s): Fotiadis D, Kanai Y, Palacin M
  • Journal Title: Mol Aspects Med. Volume: Apr;34(2-3) Issue: 2-3 Pages: 108-120
  • DOI: 10.1016/j.mam.2013.01.001
  • Peer Reviewed
• [Journal Article] L-type amino acid transporter I expression is highly correlated with Gleason score in prostate cancer. (2013)
  • Author(s): Segawa A, Nagamori S, Kanai Y, Masawa N, Oyama T.
  • Journal Title: Mol Clin One Volume: 1 Issue: 2 Pages: 274-280
  • DOI: 10.3892/mco.2012.54
  • Peer Reviewed
• [Journal Article] The Concise Guide to PHARMACOLOGY (2013)
  • Author(s): Alexander SP, Benson HE, Faccenda E, Pawson AJ, Sharman JL, 他75名
  • Journal Title: Br J Pharmacol Volume: 170 Issue: 8 Pages: 1449-1458
  • DOI: 10.1111/bph.12444
  • Peer Reviewed
• [Journal Article] Diagnostic usefulness of F-FAMT PET and L-type amino acid transporter 1 (LAT1) expression in oral squamous cell carcinoma. (2013)
  • Author(s): Nobusawa A, Kim M, Kaira K, Miyashita G, Negishi A, Oriuchi N, Higuchi T, Tsushima Y, Kanai Y, Yokoo S, Oyama T.
  • Journal Title: Eur J Nucl Med Mol Volume: 40 Issue: 11 Pages: 1692-1700
  • DOI: 10.1007/s00259-013-2477-9
  • Peer Reviewed
• [Journal Article] Clinicopathological significance of L-type amino acid transporter 1 (LAT1) expression in patients with adenoid cystic carcinoma. (2013)
  • Author(s): Kaira K, Toyoda M, Shino M, Sakakura K, Takahashi K, Tominaga H, Oriuchi N, Kanai Y, Oyama T, Chikamatsu K.
  • Journal Title: Pathol. Oncol. Res. Volume: 19 Issue: 4 Pages: 649-656
  • DOI: 10.1007/s12253-013-9624-2
  • Peer Reviewed
• [Journal Article] Biological evaluation of 3-[18F]fluoro-α-methyl-D-tyrosine (D-[18F]FAMT) as a novel amino acid tracer for positron emission tomography. (2013)
  • Author(s): Ohshima Y
  • Journal Title: Ann Nucl Med Volume: 27 Issue: 4 Pages: 314-324
  • DOI: 10.1007/s12149-013-0687-7
  • Peer Reviewed
• [Journal Article] IUPHAR-DB: updated database content and new features. (2013)
  • Author(s): Sharman JL, Benson HE, Pawson AJ, Lukito v, Mpamhanga CP, Bombail v, Davenport AP, Peters JA, Spedding M, Harmar AJ, NC-IUPHAR; Alexander SP, Bonner TI, Catterall WA, Christopoulos A, Davenport AP, Dollery CT, Enna S, Harmar AJ, Kaibuchi K, Kanai Y, Laudet v, Neubig RR, Ohlstein EH, Peters JA, Pin JP, Ruegg U, Spedding M, Wright M W, du Souich P.
  • Journal Title: Nucleic Acids Res Volume: 41 Issue: D1 Pages: D1083-D1088
  • DOI: 10.1093/nar/gks960
  • Peer Reviewed
• [Journal Article] Mammalian target of rapamycin signalling modulates amino acid upta ke by regulating transporter cell surface abundance in primary human trophoblast cells. (2013)
  • Author(s): Rosario FJ, Kanai Y, Powell T L.Jansson T
  • Journal Title: J Physiol Volume: 591 Issue: 3 Pages: 609-625
  • DOI: 10.1113/jphysiol.2012.238014
  • Peer Reviewed
• [Journal Article] NRFL-1, the C. elegans NHERF Orthologue, Interacts with Amino Acid Transporter 6 (AAT-6) for Age-Dependent Maintenance of AAT-6 on the Membrane. (2012)
  • Author(s): Kohei Hagiwara
  • Journal Title: Plos one Volume: 7(8) Issue: 8 Pages: e43050-e43050
  • DOI: 10.1371/journal.pone.0043050
  • Peer Reviewed
• [Journal Article] Establishment of Stable Cell Lines With High Expression of Heterodimers of Human 4F2hc and Human Amino Acid Transporter LAT1 or LAT2 and Delineation of Their Differential Interaction With <I>&alpha;</I>-Alkyl Moieties (2012)
  • Author(s): Narakorn Khunweeraphong
  • Journal Title: Journal of Pharmacological Sciences Volume: 119 Issue: 4 Pages: 368-380
  • DOI: 10.1254/jphs.12124FP
  • NAID: 10031071638
  • ISSN: 1347-8613, 1347-8648
  • Peer Reviewed
• [Journal Article] Prognostic significance of L-type amino-acid transporter 1 expression in surgically resected pancreatic cancer. (2012)
  • Author(s): Kaira K
  • Journal Title: Br J Cancer Volume: 107 Issue: 4 Pages: 632-638
  • DOI: 10.1038/bjc.2012.310
  • Peer Reviewed
• [Journal Article] Transport of 3-fluoro-L-α-methyl-tyrosine by tumor-upregulated L-type amino acid transporter 1: a cause of the tumor uptake in PET. (2012)
  • Author(s): Wiriyasermkul P, Nagamori S, Tominaga H, Oriuchi N, Kaira K, Nakao H, Kitashoji T, Ohgaki R, Tanaka H, Endou H, Endo K, Sakurai H, Kanai Y.
  • Journal Title: J Nucl Med. Volume: 53 Issue: 8 Pages: 1253-1261
  • DOI: 10.2967/jnumed.112.103069
  • Peer Reviewed
• [Journal Article] Linkage of N-cadherin to multiple cytoskeletal elements revealed by a proteomic approach in hippocampal neurons. (2012)
  • Author(s): Tanaka H, Takafuji K, Taguchi A, Wiriyasermkul P, Ohgaki R, Nagamori S, Suh PG, Kanai Y.
  • Journal Title: Neurochem Int. Volume: 61 Issue: 2 Pages: 240-250
  • DOI: 10.1016/j.neuint.2012.05.008
  • Peer Reviewed
• [Journal Article] How to use the IUPHAR receptor database to navigate pharmacological data. (2012)
  • Author(s): Mpamhanga CP, S harm an JL, Harmar AJ; NC-IUPHAR; Alexander SP, Bonner TI, Catterall WA, Christopoulos A, Davenport AP, Dollery CT, Enna S, Harmar AJ, Kaibuchi K, Kanai Y, Laudet V, Neubig RR, Oh I stein EH, Peters J, Pin JP, Ruegg U, du Souich P, Spedding M, Wright MW.
  • Journal Title: Methods Mol Biol. Volume: 897 Pages: 15-29
  • DOI: 10.1007/978-1-61779-909-9_2
  • ISBN: 9781617799082, 9781617799099
  • Peer Reviewed
• [Journal Article] アミノ酸トランスポーターを標的とした癌デリバリー． (2012)
  • Author(s): 金井好克
  • Journal Title: Drug Delivery System. Volume: 27 Pages: 342-349
  • NAID: 130003368325
• [Journal Article] アミノ酸トランスポーターとがん代謝 (2012)
  • Author(s): 金井好克
  • Journal Title: 実験医学 Volume: 30 Pages: 2494-2500
• [Journal Article] 悪性腫瘍の診断と治療の分子標的としてのトランスポーター. (2012)
  • Author(s): 金井好克
  • Journal Title: 細胞工学 Volume: 31 Pages: 567-571
• [Journal Article] 膜輸送系の異常概論 (2012)
  • Author(s): 金井好克
  • Journal Title: 日本臨床別冊新領域別症候群シリーズ Volume: 20 Pages: 759-766
• [Presentation] 網羅的定量オミクスによるトランスポーター研究の新しい展開Comprehensive and quantitative "omics" bring up new world of transporter research (2014)
  • Author(s): 永森收志,金井好克
  • Organizer: 2014年度日本農芸化学会シンポジウム「トランスポーターを通して見る生命現象」
  • Place of Presentation: 東京
  • Year and Date: 2014-03-30
  • Invited
• [Presentation] トランスポーターの網羅的比較定量プロテオミクス技術の確立とショ糖摂取による小腸上皮トランスポーター発現変動解析への適用 (2014)
  • Author(s): 西山俊,奥田傑,高藤和輝,ウォンタイプリンティップ,五十棲規嘉,永森收志,金井好克
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 東京
  • Year and Date: 2014-03-29
• [Presentation] Expression and functional profiles of amino acid transporters in cancer cell lines (2013)
  • Author(s): Pattama Wiriyasermkul, Shushi Nagamori, Noriyoshi Isozumi, Ryuichi Ohgaki, Hideyuki Tominaga and Yoshikatsu Kanai
  • Organizer: 第86回日本生化学会
  • Place of Presentation: 横浜
  • Year and Date: 2013-09-12
• [Presentation] がん細胞のアミノ酸トランスポーター (2013)
  • Author(s): 永森收志,金井好克
  • Organizer: 第31回日本ヒト細胞学会学術集会シンポジウム「分子標的診断・治療の進歩と今後」
  • Place of Presentation: 所沢
  • Year and Date: 2013-08-10
  • Invited
• [Presentation] The role of membrane transport in tumor cell growth and metabolism (2013)
  • Author(s): Yoshikatsu Kanai
  • Organizer: XXXVII International Congress of Physiological Sciences (IUPS 2013)
  • Place of Presentation: Birmingham, UK
  • Year and Date: 2013-07-24
  • Invited
• [Presentation] Transportsome", the multi-molecular assemblies for transport-transport and transport-metabolism couplings (2013)
  • Author(s): Yoshikatsu Kanai, Shushi Nagamori
  • Organizer: 2nd HDP international symposium on Multi-Level Systems Biology
  • Place of Presentation: 東京
  • Year and Date: 2013-06-29
  • Invited
• [Presentation] アミノ酸輸送体LAT1によって取り込まれたロイシンに対する細胞応答のリン酸プロテオミクスによる解析 (2013)
  • Author(s): 永森收志, Pattama Wiriyasermkul,世良田聡,仲哲治,金井好克
  • Organizer: 日本アミノ酸学会第7回学術大会
  • Place of Presentation: 熊本
• [Presentation] アミノ酸トランスポーターと細胞内アミノ酸シグナリング (2013)
  • Author(s): 永森收志
  • Organizer: 生理学研究所研究会「細胞センサーの分子機構・相互関連・ネットワーク研究会」
  • Place of Presentation: 岡崎
  • Invited
• [Presentation] アミノ酸トランスポーターと細胞内アミノ酸シグナリング. (2013)
  • Author(s): 永森 收志
  • Organizer: 生理学研究所研究会「細胞センサーの分子機構・相互関連・ネットワーク研究会」
  • Place of Presentation: 岡崎
  • Invited
• [Presentation] Age-dependent maintenance of transportsome in C. elegans. (2013)
  • Author(s): Kohei Hagiwara, Shushi Nagamori, Yasuhiro M. Umemura, Ryuichi Ohgaki, Yoshikatsu Kanai
  • Organizer: 第1回 Awaji International Workshop on “Electron Spin Science & Technology: Biological and Materials Science Oriented Applications
  • Place of Presentation: 淡路
• [Presentation] Substrate recognition by L-type amino acid transporter 1 (LAT1) and the important moieties of LAT1 substrates to regulate cell growth. (2013)
  • Author(s): Pattama Wiriyasermkul, Shushi Nagamori, Naoto Kojima, Yoshikatsu Kanai
  • Organizer: ordon Research Conferences 2013: Mechanisms of membrane transport
  • Place of Presentation: South Hadley, MA, USA
• [Presentation] A novel cystine transporter in S3 segment of the renal proximal tubule is an “unknown-partner” of Slc3a1 (rBAT). (2013)
  • Author(s): Shushi Nagamori, Pattama Wiriyasermkul, Meritxell Espino Guarch, Kazuhisa Okuyama, Saya Nakagomi, Kenijiro Tadagaki, Kazuaki Takafuji, Ryuichi Ohgaki, Manuel Palacín, Yoshikatsu Kanai
  • Organizer: 2nd HDP international symposium on Multi-Level Systems Biology
  • Place of Presentation: 東京
• [Presentation] 日本人型シスチン尿症を発症させるアミノ酸トランスポーター変異体の機能解析. (2013)
  • Author(s): 永森 收志、河本 泰治、Pattama Wiriyasermkul、金井 好克
  • Organizer: 生理学研究所研究会「上皮膜輸送の多層的コントロールによる生体の恒常性維持機構」
  • Place of Presentation: 岡崎
• [Presentation] Midgestational lethality in mice lacking LAT1, large neutral amino acid transporter 1, is associated with abnormal blood vessel formation and defective placental development. (2013)
  • Author(s): 大垣 隆一、李 悦、原 沙織、中込 紗綾、永森 收志、金井 好克
  • Organizer: 第86回日本生化学会
  • Place of Presentation: 横浜
• [Presentation] Expression and functional profiles of amino acid transporters in cancer cell lines. (2013)
  • Author(s): Pattama Wiriyasermkul, Shushi Nagamori, Noriyoshi Isozumi, Ryuichi Ohgaki, Hideyuki Tominaga and Yoshikatsu Kanai
  • Organizer: 第86回日本生化学会
  • Place of Presentation: 横浜
• [Presentation] アミノ酸輸送体LAT1によって取り込まれたロイシンに対する細胞応答のリン酸プロテオミクスによる解析. (2013)
  • Author(s): 永森收志、Pattama Wiriyasermkul、世良田聡、仲哲治、金井好克
  • Organizer: 日本アミノ酸学会第７回学術大会
  • Place of Presentation: 熊本
• [Presentation] Involvement of amino acid transporter in vascular endothelial cell tube formation. (2013)
  • Author(s): Saori Hara, Ryuichi Ohgaki, Printip Wongthai, Saya Nakagomi, Shushi Nagamori, Yoshikatsu Kanai
  • Organizer: 生理学研究所研究会「細胞センサーの分子機構・相互関連・ネットワーク研究会」
  • Place of Presentation: 岡崎
• [Presentation] Substrate recognition of L-type amino acid transporter 1 provide the understanding of amino acid-mediated cancer cell growth regulation. (2013)
  • Author(s): Pattama Wiriyasermkul, Shushi Nagamori, Naoto Kojima, Yoshikatsu Kanai.
  • Organizer: Nagoya Symposium; Frontiers in Structural Physiology.
  • Place of Presentation: Nagoya, Japan.
• [Presentation] Cellular responses to LAT1-mediated leucine uptake revealed by comprehensive phosphoproteomics (2012)
  • Author(s): Pattama Wiriyasermkul, Shushi Nagamori, Satoshi Serada, Masaki Matsumoto, Keiichi I Nakayama, Tetsuji Naka, Yoshikatsu Kanai
  • Organizer: 2012 American Society of Cell Biology (ASCB) Annual Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2012-12-17
• [Presentation] アミノ酸トランスポーターLAT1を介したロイシンの取り込みとmTOR系活性化の網羅的解析 (2012)
  • Author(s): 永森收志、Pattama Wiriyasermkul、世良田聡、仲哲治、金井好克
  • Organizer: 日本アミノ酸学会第6回学術大会
  • Place of Presentation: 松戸
• [Presentation] アミノ酸トランスポーターLAT1を介したロイシンの取り込みとmTOR系活性化の網羅的解析. (2012)
  • Author(s): 永森收志、Pattama Wiriyasermkul、世良田聡、仲哲治、金井好克
  • Organizer: 日本アミノ酸学会第６回学術大会
  • Place of Presentation: 千葉県松戸市
• [Presentation] Transportsome”, the multi-molecular assemblies for transport-transport and transport-metabolism couplings.
  • Author(s): Yoshikatsu Kanai, Shushi Nagamori
  • Organizer: 2nd HDP international symposium on Multi-Level Systems Biology
  • Place of Presentation: 東京
  • Invited
• [Presentation] アミノ酸トランスポーターを利用した癌へのドラッグデリバリー.
  • Author(s): 金井 好克
  • Organizer: 第29回日本DDS学会学術集会
  • Place of Presentation: 京都
  • Invited
• [Presentation] Amino acid transporters in cancers for effective and selective delivery of cancer-targeting agents.
  • Author(s): Yoshikatsu Kanai
  • Organizer: BioMedical Transporters 2013
  • Place of Presentation: St. Moritz, Switzerland
  • Invited
• [Presentation] がんの診断・治療の分子標的としてのアミノ酸トランスポーター.
  • Author(s): 金井 好克
  • Organizer: 金沢大学『Transcriptotherapeuticsの創出と医療への展開』セミナー
  • Place of Presentation: 金沢
  • Invited
• [Presentation] アミノ酸トランスポーターと疾患.
  • Author(s): 金井 好克
  • Organizer: 第86回日本生化学会大会 シンポジウム「トランスポーターと疾患の最前線」
  • Place of Presentation: 横浜
  • Invited
• [Presentation] トランスポーターの機能と病態.
  • Author(s): 金井 好克
  • Organizer: 第23回国際痒みシンポジウム
  • Place of Presentation: 大阪
  • Invited
• [Presentation] トランスポーターシステムの分子的描像と疾患治療への可能性を求めて
  • Author(s): 金井 好克
  • Organizer: Advans研究会
  • Place of Presentation: 東京
  • Invited
• [Presentation] アミノ酸トランスポーターを標的とした抗腫瘍薬の創製.
  • Author(s): 金井 好克
  • Organizer: 第98回岡山県医用工学研究会シンポジウム
  • Place of Presentation: 岡山
  • Invited
• [Presentation] アミノ酸トランスポーターを標的とした悪性腫瘍のPET診断と治療薬の開発.
  • Author(s): 金井 好克
  • Organizer: 第87回日本薬理学会年会 シンポジウム「アカデミアにおける創薬とトランスレーショナルリサーチの展望」
  • Place of Presentation: 仙台
  • Invited
• [Presentation] がん細胞のアミノ酸取り込みを標的とした新しい作用機序の抗がん薬～難治性がんの新薬～
  • Author(s): 金井 好克
  • Organizer: 大阪大学未来戦略機構シンポジウム アカデミア基盤研究から未来創薬へ
  • Place of Presentation: 東京
  • Invited
• [Presentation] がん細胞のアミノ酸トランスポーター.
  • Author(s): 永森收志、金井好克
  • Organizer: 第31回日本ヒト細胞学会学術集会 シンポジウム「分子標的診断・治療の進歩と今後」
  • Place of Presentation: 所沢
  • Invited
• [Presentation] 網羅的定量オミクスによるトランスポーター研究の新しい展開Comprehensive and quantitative "omics" bring up new world of transporter research.
  • Author(s): 永森收志、金井好克
  • Organizer: 2014年度日本農芸化学会 シンポジウム「トランスポーターを通して見る生命現象」
  • Place of Presentation: 東京
  • Invited
• [Presentation] 線虫腸管細胞におけるアミノ酸トランスポーターAAT-6の頂端膜局在に寄与するトランスポートソーム.
  • Author(s): 村田 大輔、大垣 隆一、萩原 浩平、Kanokporn Phetdee、永森 收志、平田 拓、野村 和子、野村 一也、三谷 昌平、金井 好克
  • Organizer: 第8回トランスポーター研究会年会
  • Place of Presentation: 熊本
• [Presentation] 3-Fluoro-L-a-methyltyrosine (FAMT), a positron emission tomography probe for cancer diagnosis, is a selective substrate of cancer-specific amino acid transporter L-type amino acid transporter 1 (LAT1).
  • Author(s): Ling Wei、富永 英之、Pattama Wiriyasermkul、大垣 隆一、忠垣 憲次郎、永森 收志、金井 好克
  • Organizer: 第123回日本薬理学会近畿部会
  • Place of Presentation: 名古屋
• [Presentation] 疾患起因性変異ノックインマウスを用いた日本人型シスチン尿症変異の特性解析.
  • Author(s): 河本 泰治、永森 收志、中込 咲綾、Pattama Wiriyasermkul、金井 好克
  • Organizer: 第四回分子腎臓フォーラム
  • Place of Presentation: 京都
• [Presentation] 原 早織、大垣 隆一、Printip Wongthai、中込 咲綾、永森 收志、金井 好克：アミノ酸トランスポーターLAT1阻害による血管内皮細胞 チューブ形成への影響についての検討.
  • Author(s): 原 早織、大垣 隆一、Printip Wongthai、中込 咲綾、永森 收志、金井 好克
  • Organizer: 第124回日本薬理学会近畿部会
  • Place of Presentation: 京都
• [Presentation] Transport of 4-borono-L-phenylalanine, a 10B carrier of boron neutron capture therapy, by system L amino acid transporters.
  • Author(s): Printip Wongthai, Kohei Hagiwara, Yurika Miyoshi, Pattama Wiriyasermkul, Ryuichi Ohgaki, Kenjiro Tadagaki, Kenji Hamase, Shushi Nagamori, Yoshikatsu Kanai
  • Organizer: 第124回日本薬理学会近畿部会
  • Place of Presentation: 京都
• [Presentation] 線虫アミノ酸トランスポーターAAT-6の輸送機能および細胞内局在の解析.
  • Author(s): 村田 大輔、大垣 隆一、萩原 浩平、Kanokporn Phetdee、永森 收志、魏 玲、平田 拓、野村 一也、三谷 昌平、金井 好克
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Mechanisms of tumor cell uptake of 4-borono-phenylalanine, a 10B carrier of boron neutron capture therapy, mediated by system L amino acid transporters.
  • Author(s): Printip Wongthai, Kohei Hagiwara, Yurika Miyoshi, Pattama Wiriyasermkul, Pornparn Kongpracha, Isozumi Noriyoshi, Ryuichi Ohgaki, Kenjiro Tadagaki, Kenji Hamase, Shushi Nagamori, Yoshikatsu Kanai
  • Organizer: 第87回日本薬理学会年会
  • Place of Presentation: 仙台
• [Presentation] Evaluation of amino acid transporter LAT1 as a molecular target of anti-tumor therapy by means of the combination of comprehensive proteomics and transport assay.
  • Author(s): 永森 收志、Pattama Wiriyasermkul、五十棲 規嘉、Pornparn Kongpracha、大垣 隆一、金井 好克
  • Organizer: 第87回日本薬理学会年会
  • Place of Presentation: 仙台
• [Presentation] トランスポーターの網羅的比較定量プロテオミクス技術の確立とショ糖摂取による小腸上皮トランスポーター発現変動解析への適用.
  • Author(s): 西山 俊、奥田 傑、高藤 和輝、ウォンタイ プリンティップ、五十棲 規嘉、永森 收志、金井 好克
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 東京
• [Presentation] “Transportsome”, the multi-molecular assemblies for transport-transport and transport-metabolism couplings.
  • Author(s): Yoshikatsu Kanai
  • Organizer: International Symposium on Epithelial Barrier and Transport 2012.
  • Place of Presentation: Shiga, Japan.
• [Presentation] Comprehensive variability analysis of nutrients absorption mechanism in small intestine by quantitative proteomics.
  • Author(s): Takashi Nishiyama , Shushi Nagamori , Kazuaki Takafuji , Yoshikatsu Kanai．
  • Organizer: 第19回国際質量分析会議.
  • Place of Presentation: 京都市
• [Presentation] アミノ酸トランスポーターLAT1の発見と新たな分子標的としての意義．
  • Author(s): 金井 好克
  • Organizer: がん診療とPETに関するセミナー＠佐久総合病院「アミノ酸トランスポーターを標的としたがんのPET診断と治療応用」
  • Place of Presentation: 長野県佐久市
• [Presentation] Comprehensive phosphoproteomics analysis of cellular responses induced by LAT1-mediated leucine uptake.
  • Author(s): Pattama Wiriyasermkul, 永森 收志, 世良田 聡, 仲 哲治, 金井 好克
  • Organizer: 第122回日本薬理学会近畿部会
  • Place of Presentation: 大阪府豊中市
• [Presentation] Metabolomics and proteomics in transporter research（トランスポーター研究におけるメタボロームとプロテオーム解析）
  • Author(s): 金井好克
  • Organizer: 日本薬物動態学会第27回年会シンポジウム「トランスポーターの生理機能：統合的理解に向けて」
  • Place of Presentation: 東京
• [Presentation] 悪性腫瘍のアミノ酸トランスポーター
  • Author(s): 金井好克
  • Organizer: 第85回日本生化学会大会シンポジウム「分子標的トランスポーター」
  • Place of Presentation: 福岡市
• [Presentation] アミノ酸トランスポーターLAT1阻害によるがん細胞増殖抑制．
  • Author(s): 西中由美子、永森收志、 Printip Wongthai、遠藤 仁、金井好克
  • Organizer: 第85回日本生化学会大会．
  • Place of Presentation: 福岡市
• [Presentation] Cellular responses to LAT1-mediated leucine uptake revealed by comprehensive phosphoproteomics.
  • Author(s): Pattama Wiriyasermkul, Shushi Nagamori, Satoshi Serada, Masaki Matsumoto, Keiichi I Nakayama, Tetsuji Naka, Yoshikatsu Kanai.
  • Organizer: 2012 American Society of Cell Biology (ASCB) Annual Meeting.
  • Place of Presentation: San Francisco, USA
• [Presentation] A C-terminus mutation of SLC7A9 amino acid transporter frequent in Japanese cystinuria patients retains substrate binding but diminishes the transport activity.
  • Author(s): Y. Kawamoto, S. Nagamori, P. Wiriyasermkul, Y. Kanai.
  • Organizer: 2012 American Society of Cell Biology (ASCB) Annual Meeting.
  • Place of Presentation: San Francisco, USA.
• [Presentation] A C-terminus mutation of b0,+AT/SLC7A9 amino acid transporter frequent in Japanese cystinuria patients binds substrates but diminishes the transport activity.
  • Author(s): Yasuharu Kawamoto, Shushi Nagamori, Saya Nakagomi, Pattama Wiriyasermkul, Yoshikatsu Kanai.
  • Organizer: Nagoya Symposium; Frontiers in Structural Physiology.
  • Place of Presentation: Nagoya, Japan.
• [Presentation] Kinetic analysis of 3F-L-α-methyltyrosine (FAMT) transport by L-type amino acid transporter 1.
  • Author(s): Ling Wei, Hideyuki Tominaka, Pattama Wiriyasermkul, Yumiko Nishinaka, Ryuichi Ohgaki, Kenjiro Tadagaki, Shushi Nagamori, Yoshikatsu Kanai
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡市
• [Presentation] Boronophenylalanine; a rational carrier for 10B for BNCT, is transported by both LAT1 and LAT2.
  • Author(s): Printip Wongthai, Pattama Wiriyasermkul, Yumiko Nishinaka, Ryuichi Ohgaki, Kenjiro Tadagaki, Shushi Nagamori, Yoshikatsu Kanai.
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡市
• [Presentation] Cellular responses to LAT1-mediated leucine uptake are revealed by using an LAT1 inhibitor and comprehensive phosphoproteomics.
  • Author(s): Shushi Nagamori, Pattama Wiriyasermkul, Satoshi Serada, Masaki Matsumoto, Keiichi I Nakayama, Tetsuji Naka, Yoshikatsu Kanai
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡市
• [Book] トランスポートソーム：その生理的意義と疾患 (2013)
  • Author(s): 金井好克
  • Total Pages: 8
  • Publisher: 中外医学社
• [Remarks]
  • URL: http://www.pharma1.med.osaka-u.ac.jp/