Identification of transcription regulatory factor using chromatinized DNA template immobilized on microbeads

• Project/Area Number: 24659121
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Hokkaido University
• Principal Investigator: TAKAHASHI Hidehisa 北海道大学, 医学(系)研究科(研究院), 助教 (30423544)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 転写因子 / 転写制御因子 / HNF4 alpha / ES細胞 / HNF4alpha

Research Abstract

Many evidence including that all of the Yamanaka factors necessary for generation of IPS cells are transcription factors indicates that transcription factors play an very important role in regulation of gene expression. To address the mechanism how transcription factor regulates gene transcription, we performed the identification of transcription regulatory factor using chromatinized DNA template.

Research Products

• [Journal Article] The TRIM-FLMN protein TRIM45 directly interacts with RACK1 and negatively regulates PKC-mediated signaling pathway (2014)
  • Author(s): Sato T., Takahashi H., Hatakeyama S., Iguchi A. and Ariga T.
  • Journal Title: Oncogene Volume: 31;0
  • Peer Reviewed
• [Journal Article] The TRIM-FLMN protein TRIM45 directly interacts with RACK1 and negatively regulates PKC-mediated signaling pathway. (2014)
  • Author(s): Sato T., Takahashi H., Hatakeyama S., Iguchi A. and Ariga T
  • Journal Title: Oncogene Volume: In press
  • Peer Reviewed
• [Journal Article] Identification of anti-Sez6l2 antibody in a patient with cerebellar ataxia and retinopathy (2013)
  • Author(s): Yaguchi H, Yabe I, Takahashi H, Okumura F, Takeuchi A, Horiuchi, K, Kan T, Kanda, A, Saito W, Matsumoto M, Nakayama KI, Hatakeyama S and Sasaki H
  • Journal Title: J. Neurol. Volume: 261 Issue: 1 Pages: 224-226
  • DOI: 10.1007/s00415-013-7134-5
  • Peer Reviewed
• [Journal Article] Transcriptional elongation factor elongin A regulates retinoic acid-induced gene expression during neuronal differentiation. (2012)
  • Author(s): Yasukawa T, Bhatt S, Takeuchi T, Kawauchi J, Takahashi H, Tsutsui A, Muraoka T, Inoue M, Tsuda M, Kitajima S, Conaway RC, Conaway JW, Trainor PA, Aso T.
  • Journal Title: Cell Rep Volume: 2(5) Issue: 5 Pages: 1129-36
  • DOI: 10.1016/j.celrep.2012.09.031
  • Peer Reviewed
• [Journal Article] TRIM67 protein negatively regulates Ras activity through degradation of 80K-H and indices neuritogenesis (2012)
  • Author(s): Yaguchi, H., Okumura, F., Takahashi, H., Kano, T., Kameda, H., Uchigashima, M., Tanaka, S., Watanabe, M., Sasaki, H. and Hatakeyama, S.
  • Journal Title: J. Biol. Chem. Volume: 287 Issue: 15 Pages: 12050-12059
  • DOI: 10.1074/jbc.m111.307678
  • Peer Reviewed
• [Journal Article] Role for the human mediator subunit Med26 in transcription elongation (2012)
  • Author(s): Takahashi H
  • Journal Title: Seikagaku Volume: 84(7) Pages: 577-581
  • NAID: 10030966779
  • Peer Reviewed
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2013)
  • Author(s): 高橋秀尚,瀧川一学, Delnur Anwar,柴田美音,佐藤チエリ,佐藤滋生, Ranjan Amol, Chris W. Seidel,築山忠維,渡部昌,林正康,大川恭行, Joan W. Conaway, Ronald C. Conaway,畠山鎮次
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸ポートピアホテル(神戸)
• [Presentation] Human Mediator subunit Med26 regulates the transcription of small nuclear RNA genes through the recruitment of little elongation complex (2013)
  • Author(s): Hidehisa Takahashi, Chieri Tomomori-Sato, Shigeo Sato, Amol Ranjan, Masayasu Hayashi, Yasuyuki Ohkawa, Joan W. Conaway, Ronald C. Conaway, Shigetsugu Hatakeyama
  • Organizer: Mechanism of eukaryotic transcription, Cold spring harbor meeting
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2013)
  • Author(s): 高橋秀尚, 瀧川一学, Delnur Anwar, 柴田美音, 佐藤チエリ, 佐藤滋生, Ranjan Amol, Chris W. Seidel, 築山忠維, 渡部昌, 林正康, 大川恭行, Joan W. Conaway, Ronald C. Conaway, 畠山鎮次
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピアアイランド（神戸市）
• [Presentation] Human Mediator subunit Med26 regulates the transcription of small nuclear RNA genes through the recruitment of little elongation complex. (2013)
  • Author(s): Hidehisa Takahashi, Chieri Tomomori-Sato, Shigeo Sato, Amol Ranjan, Masayasu Hayashi, Yasuyuki Ohkawa, Joan W. Conaway, Ronald C. Conaway, Shigetsugu Hatakeyama
  • Organizer: Mechanism of eukaryotic transcription, Cold spring harbor meeting
  • Place of Presentation: Cold spring harbor laboratory (USA)
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2012)
  • Author(s): 高橋秀尚, Joan W. Conaway, Ronald C. Conaway,畠山鎮次
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場,マリンメッセ福岡(福岡)
• [Presentation] TRIM29によるTRRAP/Tip60複合体の機能制御とがん化との関わり (2012)
  • Author(s): 桝田安志,高橋秀尚,畠山鎮次
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場,マリンメッセ福岡(福岡)
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2012)
  • Author(s): 高橋秀尚,佐藤智信, Conaway, J.W., Conaway, R.C.,畠山鎮次
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場,マリンメッセ福岡(福岡)
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2012)
  • Author(s): 高橋秀尚,佐藤智信,Conaway, J.W., Conaway, R.C.,畠山鎮次 高橋秀尚,佐藤智信,Conaway, J.W., Conaway, R.C.,畠山鎮次
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡（福岡）
• [Presentation] Human Mediator subunit Med26 plays a role in recruitment of "Little elongation complex" to small nuclear RNA genes（ポスター） (2012)
  • Author(s): Hidehisa Takahashi, Joan W. Conaway, Ronald C. Conaway, Shigetsugu Hatakeyama
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡（福岡）
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2012)
  • Author(s): 髙橋秀尚、Joan W. Conaway、Ronald C. Conaway、畠山 鎮次
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡（福岡）
• [Presentation] TRIM29によるTRRAP/Tip60複合体の機能制御とがん化との関わり (2012)
  • Author(s): 桝田安志、髙橋秀尚、畠山鎮次
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡（福岡）
• [Remarks]
  • URL: http://www.hucc.hokudai.ac.jp/~d20505/takahashi/
• [Remarks] 新規転写伸長制御因子Med26の機能解析
  • URL: http://www.hucc.hokudai.ac.jp/~d20505/takahashi/index.html
• [Remarks] 新規転写伸長因子Med26の機能解析
  • URL: http://www.hucc.hokudai.ac.jp/~d20505/takahashi/