| Project/Area Number |
24659123
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
SHIBAHARA Shigeki 東北大学, 医学(系)研究科(研究院), 教授 (00154253)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEDA Kazuhisa 東北大学, 大学院医学系研究科, 准教授 (30311559)
FURUYAMA Kazumichi 岩手医科大学, 医学部, 教授 (80280874)
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIZAWA Miki 東北大学, 大学院医学系研究科, 技術専門職員 (50221669)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 網膜色素上皮 / 転写因子 / メラニン / L1エレメント / MITF / 生体防御 / ストレス応答 / 網膜色素上皮細胞 / 回避行動 |
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| Research Abstract |
Microphthalmia-associated transcription factor (Mitf) is required for differentiation of retinal pigment epithelium. The mouse homozygous for the black-eyed white (Mitf-bw) allele is characterized by white-coat color and deafness with black eye, due to the loss of melanoblasts. The Mitf-bw allele carries an insertion of L1 element in the Mitf gene, causing the deficiency of the melanocyte-specific Mitf-M. Moreover, alternatively spliced Mitf-M mRNA species are expressed in developing melanoblasts: Mitf-M mRNA lacking exon 3, exon 4, or both exons 3 and 4. These aberrant mRNAs are also expressed in the bw mouse brain. Importantly, Mitf-M mRNA is expressed in the mouse and human brain. Moreover, the bw mice show the altered volitional motor activity under a stressful environment.
We identified the MITF isoform expressed in the human retinal pigment epithelium. Moreover, the beta-catenin is responsible for maintaining the expression of MITF in the retinal pigment epithelium.
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