| Project/Area Number |
24659127
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OTSU MAKOTO 東京大学, 医科学研究所, 准教授 (30361330)
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| Co-Investigator(Kenkyū-buntansha) |
KATAYAMA Yoshiki 九州大学, 工学(系)研究科(研究院), 教授 (70284528)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 造血幹細胞 / シグナル伝達 / シグナル応答性プローブ / リン酸化 / シグナル / プローブ |
|---|
| Research Abstract |
In this project we have aimed at the development of a new method to isolate previously unidentified subfractions in hematopoietic stem cells (HSC) by utilizing their varying responsiveness to a certain cytokine signal. Intracellular markers have been developed as the fluorescent nanoparticles containing peptide substrates for certain kinases. After screening candidate peptide substrates, the synthesized marker will be introduced into HSC to detect and/or subfractionate them for characterization.
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