A new method to sub-fractionate hematopoietic stem cells based on signal response characteristics
| Project/Area Number |
24659127
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OTSU MAKOTO 東京大学, 医科学研究所, 准教授 (30361330)
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| Co-Investigator(Kenkyū-buntansha) |
KATAYAMA Yoshiki 九州大学, 工学(系)研究科(研究院), 教授 (70284528)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 造血幹細胞 / シグナル伝達 / シグナル応答性プローブ / リン酸化 / シグナル / プローブ |
|---|
| Research Abstract |
In this project we have aimed at the development of a new method to isolate previously unidentified subfractions in hematopoietic stem cells (HSC) by utilizing their varying responsiveness to a certain cytokine signal. Intracellular markers have been developed as the fluorescent nanoparticles containing peptide substrates for certain kinases. After screening candidate peptide substrates, the synthesized marker will be introduced into HSC to detect and/or subfractionate them for characterization.
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Report
(3 results)
Research Products
(34 results)
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[Journal Article] Stage-specific roles for Cxcr4 signaling in murine hematopoietic stem/progenitor cells in the process of bone marrow repopulation.2014
Author(s)
Lai CY, Yamazaki S, Okabe M, Suzuki S, Maeyama Y, Iimura Y, Onodera M, Kakuta S, Iwakura Y, Nojima M, Otsu M, Nakauchi H.
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Journal Title
Stem Cells.
Volume: -
Issue: 7
Pages: 1929-42
DOI
Related Report
Peer Reviewed
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[Journal Article] Profiling of microRNA in human and mouse ES and iPS cells reveals overlapping but distinct microRNA expression patterns.2013
Author(s)
Razak SR, Ueno K, Takayama N, Nariai N, Nagasaki M, Saito R, Koso H, Lai CY, Murakami M, Tsuji K, Michiue T, Nakauchi H, Otsu M, Watanabe S.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] CAMT-iPS Cells Exhibiting Defective MPL Signaling Dysregulate Megakaryopoiesis and Erythropoiesis.2013
Author(s)
Shinji Hirata, Naoya Takayama,Ryoko Jono-Ohnishi, Hiroshi Endo, Sou Nakamura, Takeaki Dohda, Masanori Nishi, Yuhei Hamazaki, Ei-ichi Ishii, Shin Kaneko, Makoto Otsu, Hiromitsu Nakauchi, Shinji Kunishima, and Koji Eto.
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Journal Title
J Clin Invest.
Volume: 123
Issue: 9
Pages: 3802-14
DOI
Related Report
Peer Reviewed
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