Research Project
Grant-in-Aid for Challenging Exploratory Research
We treated mammalian cells with antisense oligo for each component of spliceosome and found that all antisense oligo could inhibit splicing activity in a dose-dependent manner. Splicing inhibition by the antisense oligos decreased cell survival rate and interestingly the magnitude of the effect was different among cell lines.We also treated mouse 3T3-L1 cells with a splicing inhibitor and found that adipogenesis was defective after splicing inhibitor treatment. This inhibition was also affected the timing of addition of splicing inhibitor.
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