Study on the mechanism of tissue specific diseases caused by mRNA splicing defect
Project/Area Number |
24659129
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Single-year Grants |
Research Field |
General medical chemistry
|
Research Institution | University of Toyama |
Principal Investigator |
KAIDA DAISUKE 富山大学, 先端ライフサイエンス拠点, 特命助教 (60415122)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | mRNAスプライシング / 組織特異的疾患 / snRNA / 組織特異的遺伝子発現 / スプライシング |
Research Abstract |
We treated mammalian cells with antisense oligo for each component of spliceosome and found that all antisense oligo could inhibit splicing activity in a dose-dependent manner. Splicing inhibition by the antisense oligos decreased cell survival rate and interestingly the magnitude of the effect was different among cell lines. We also treated mouse 3T3-L1 cells with a splicing inhibitor and found that adipogenesis was defective after splicing inhibitor treatment. This inhibition was also affected the timing of addition of splicing inhibitor.
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Report
(3 results)
Research Products
(1 results)