• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Exploration of novel molecular targets for anti-cancer drug in long non-coding RNAs

Research Project

Project/Area Number 24659130
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

KITAGAWA Masatoshi  浜松医科大学, 医学部, 教授 (50294971)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsノンコーディングRNA / 癌抑制遺伝子 / INK4 locus / エピジェネティクス / 癌
Research Abstract

lncRNA ANRIL negatively regulates tumore suppressor p16Ink4 via recruitment of PRC2. We found that RNA immunoprecipitation method is applicable for a screening system to evaluate the binding between ANRIL and SUZ12, a component of PRC2. Moreover, we developed quantitation methods to measure fifteen known lncRNAs including ANRIL to evaluate their association with cancer development. Although, we evaluate them in hepatocellular carcinoma cell lines with or without HBx-expression using this system, there are no lncRNA oscillating by HBx-expression. Further evaluation will be required.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (21 results)

All 2014 2013 2012 Other

All Journal Article (13 results) (of which Peer Reviewed: 12 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Invited: 2 results) Remarks (1 results)

  • [Journal Article] YB-1 promotes transcription of cyclin D1 in human non-small-cell lung cancers2014

    • Author(s)
      Harada, M., Kotake, Y., Ohhata, T., Kitagawa, K., Niida, H., Matsuura, S., Funai, K., Sugimura, H., Suda, T. and Kitagawa, M.
    • Journal Title

      Genes Cells

      Volume: 18 Issue: 6 Pages: 999-1006

    • DOI

      10.1111/gtc.12150

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interaction between RB protein and NuMA is required for proper alignment of spindle microtubules2014

    • Author(s)
      Uchida, C., Hattori, T., Takahashi, H., Yamamoto, N., Kitagawa, M. and Taya, Y.
    • Journal Title

      Genes Cells

      Volume: 19 Issue: 2 Pages: 89-96

    • DOI

      10.1111/gtc.12119

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anti-VEGF antibody therapy induces tumor hypoxia and stanniocalcin 2 expression and potentiates growth of human colon cancer xenografts.2014

    • Author(s)
      Miyazaki S, Kikuchi H, Iino I, Uehara T, Setoguchi T, Fujita T, Hiramatsu Y, Ohta M, Kamiya K, Kitagawa K, Kitagawa M, Baba S, Konno H
    • Journal Title

      Int J Cancer

      Volume: 135 Issue: 2 Pages: 295-307

    • DOI

      10.1002/ijc.28686

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Cell cycle regulation by long non-coding RNAs.2013

    • Author(s)
      Kitagawa M, Kitagawa K, Kotake Y, Niida H, Ohhata T.
    • Journal Title

      Cell Mol Life Sci.

      Volume: 24 Issue: 24 Pages: 4785-4794

    • DOI

      10.1007/s00018-013-1423-0

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] YB1 binds to and represses the p16 tumor suppressor gene.2013

    • Author(s)
      Kotake Y, Ozawa Y, Harada M, Kitagawa K, Niida H, Morita Y, Tanaka K, Suda T, Kitagawa M.
    • Journal Title

      Genes Cells.

      Volume: 11 Issue: 11 Pages: 999-1006

    • DOI

      10.1111/gtc.12093

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] ユビキチンシステムによる細胞周期制御と疾患 腎疾患におけるユビキチンシステムの機能2013

    • Author(s)
      北川雅敏
    • Journal Title

      実験医学増刊号

      Volume: 31 Pages: 33-38

    • Related Report
      2012 Research-status Report
  • [Journal Article] Long noncoding RNA involved in cancer development and cell fate determination.2012

    • Author(s)
      Kitagawa, M., et al.
    • Journal Title

      Curr. Drug Targets

      Volume: 13 Issue: 13 Pages: 1616-1621

    • DOI

      10.2174/138945012803530026

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Roles of the Skp2/p27 axis in the progression of chronic nephropathy2012

    • Author(s)
      Suzuki, S., Ohashi, N. and Kitagawa, M
    • Journal Title

      Cell Mol Life Sci

      Volume: (in press) Issue: 18 Pages: 3277-3289

    • DOI

      10.1007/s00018-012-1232-x

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Decreased tonic inhibition in cerebellar granule cells causes motor dysfunction in a mouse model of Angelman syndrome.2012

    • Author(s)
      Egawa, K., et al.
    • Journal Title

      Sci. Transl. Med.

      Volume: 4 Issue: 163

    • DOI

      10.1126/scitranslmed.3004655

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Chkl phosphorylates the tumor suppressor Mig-6, regulating the activation of EGF signaling.2012

    • Author(s)
      Liu, N., et al.
    • Journal Title

      EMBO J.

      Volume: 31 Issue: 10 Pages: 2365-2377

    • DOI

      10.1038/emboj.2012.88

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The SCF ubiquitin ligases involved in hematopoietic linage.2012

    • Author(s)
      Kitagawa, K., Kitagawa, M.
    • Journal Title

      Curr. Drug Targets

      Volume: 13 Issue: 13 Pages: 1641-1648

    • DOI

      10.2174/138945012803529974

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Regulation of DNA replication licensing.2012

    • Author(s)
      Niida, H., Kitagawa, M.
    • Journal Title

      Curr. Drug Targets

      Volume: 13 Issue: 13 Pages: 1588-1592

    • DOI

      10.2174/138945012803529965

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The amelioration of renal damage in Skp2-deficient mice is canceled by p27 Kipl deficiency in Skp2^-/- p27^-/- mice.2012

    • Author(s)
      Suzuki, S., et al.
    • Journal Title

      PLoS ONE

      Volume: 13 Issue: 4 Pages: e36249-e36249

    • DOI

      10.1371/journal.pone.0036249

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] マウスES細胞におけるXist抑制機構解析2013

    • Author(s)
      大畑樹也、松本美香、Martin Leeb,柴田進和、廣瀬哲郎、北川恭子、丹伊田浩行、北川雅敏、Anton Wutz
    • Organizer
      第36回日本分子生物学会年会ポスター発表
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] Investigation of long non-coding RNAs involved in regulating X-chromosome inactivation2013

    • Author(s)
      Tatsuya Ohhata, Masatoshi Kitagawa
    • Organizer
      The 13th Hamamatsu- Kyungpook Joint Medical Symposium, Plenary talk
    • Place of Presentation
      Deagu, Korea
    • Related Report
      2013 Final Research Report
  • [Presentation] Molecular dissection of Xist repression through Tsix dependent/independent manner in undifferentiated ES cells2013

    • Author(s)
      Tatsuya Ohhata, Mika Matsumoto, Martin Leeb, Shinwa Shibata, Tesuro Hirose, Masatoshi Kitatgawa, Anton Wutz
    • Organizer
      Gordon research conference, Epigenetics
    • Place of Presentation
      Rhode Island, USA
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Investigation of long non-coding RNAs involved in regulating X-chromosome inactivation2013

    • Author(s)
      Tatsuya Ohhata, Masatoshi Kitagawa
    • Organizer
      The 13th Hamamatsu-Kyungpook Joint Medical Symposium
    • Place of Presentation
      Deagu, Korea
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Recent progress of Long non-coding RNAs research2012

    • Author(s)
      大畑樹也、北川雅敏
    • Organizer
      第71回日本癌学会年会シンポジウム発表
    • Place of Presentation
      札幌
    • Related Report
      2013 Final Research Report
  • [Presentation] Recent Progress of long non-coding RNA research

    • Author(s)
      大畑樹也
    • Organizer
      第71回日本癌学会総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Chk1 phosphorylates the tumor suppressor Mig-6, regulating the activation of EGF signaling.

    • Author(s)
      北川雅敏
    • Organizer
      第45回日本発生生物学会、第64回日本細胞生物学会合同年会
    • Place of Presentation
      神戸
    • Related Report
      2012 Research-status Report
  • [Remarks] 浜松医科大学医学部 分子生物学講座

    • URL

      http://www.hama-med.ac.jp/uni_education_igakubu_igaku_seika1.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi