Search for the endogenous ligad based on the orphan receptor structure
| Project/Area Number |
24659132
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
KOBAYASHI Takuya 京都大学, 医学(系)研究科(研究院), 准教授 (20311730)
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| Co-Investigator(Renkei-kenkyūsha) |
IWATA So 京都大学, 医学研究科, 教授 (60452330)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | モノクローナル抗体 / GPCR / X線結晶構造解析 / オーファン受容体 / 抗体 |
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| Outline of Final Research Achievements |
G protein coupled receptors (GPCRs) are nature’s most versatile chemical sensors. There are over 600 GPCRs in the human genome and they respond to a broad spectrum of chemical entities ranging from photons, protons and calcium ions, to small organic molecules (including odorants and neurotransmitters), to peptides and glycoproteins. However, for 25% of GPCRs, endogenous ligands have not yet been identified. We would like to determine the structure of orphan receptor and search for the endogenous ligands based on these structures. To determine the structure of orphan receptor, we have tried to develop the monoclonal antibody recognizing the conformational epitope of receptor. As a result, several monoclonal antibodies recognizing the sequential epitope of orphan receptors have been screened.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Proteoliposome-based selection of a recombinant antibody fragment against the human M2 muscarinic acetylcholine receptor.2014
Author(s)
Suharni, Nomura Y, Arakawa T, Hino T, Abe H, Nakada-Nakura Y, Sato Y, Iwanari H, Shiroishi M, Asada H, Shimamura T, Murata T, Kobayashi T, Hamakubo T, Iwata S, Nomura N.
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Journal Title
Monoclon Antib Immunodiagn Immunother
Volume: 33
Issue: 6
Pages: 378-385
DOI
NAID
Related Report
Peer Reviewed
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