| Project/Area Number |
24659135
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
NAKANO Toru 大阪大学, 生命機能研究科, 教授 (00172370)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | DNAメチル化 / 遺伝子発現制御 / 生殖細胞 / エピジェネティクス / 発生・分化 / 精子形成 / 国際情報交換 |
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| Research Abstract |
We planned to establish two novel strategies to introduce artificial DNA methylation using zinc finger fusion proteins and small RNAs. One is the introduction of de novo DNA methylation in embryonic male germ cells by using the fusion protein of zinc finger protein and MIWI2 protein. The other is the introduction of DNA methylation in culture cells by using the triplex nucleic acids consisting of two DNA strands and one RNA strand. Although, unfortunately, the latter experiment did not work, we succeeded in establishing the latter one. Following the success, we are currently analyzing the function of MIWI2 in the embryonic male germ cells.
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