Search for biomarkers of successful aging through glycomics and glycoproteomics of semisuper centenarians

• Project/Area Number: 24659141
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology
• Principal Investigator: ENDO Tamao 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 副所長 (30168827)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: プロテオミクス / 超百寿者 / 健康長寿マーカー / 糖鎖解析 / レクチンマイクロアレイ / 糖タンパク質 / LC-MS / グライコプロテオミクス / LC-MS/MS

Research Abstract

The purpose of this study is to clarify the candidates of the biomarker of successful aging through glycomics and glycoproteomics of semisuper centenarians considered as a model of human longevity. I performed sugar chains analyses, lectin microarray analyses, and glycopeptides analyses, and compared the glycosylation of plasma proteins between semisuper centenarians and controls. As a result, multi-branched complex sugar chains and high sialylated sugar chains were increased in the plasma proteins of semisuper centenarians. Sialylated glycopeptides of inflammatory mediating proteins were decreased with aging, while not in semisuper centenarians. It was suggested that the glycosylation plays an important role in successful aging of semisuper centenarians.

Research Products

• [Journal Article] 健康長寿への挑戦ー長寿者プロテオミクスが明らかにするものとはー (2013)
  • Author(s): 三浦ゆり、遠藤玉夫
  • Journal Title: 基礎老化研究 Volume: 37 Pages: 23-27
  • Peer Reviewed
• [Journal Article] 健康長寿への挑戦 -長寿者プロテオミクスが明らかにするものとは- (2013)
  • Author(s): 遠藤玉夫、三浦ゆり
  • Journal Title: 基礎老化研究 Volume: 37 Pages: 23-27
  • Peer Reviewed
• [Presentation] 日本人超百寿者血漿タンパク質のグライコプロテオミクス解析 (2014)
  • Author(s): 三浦ゆり、太田悠葵、高倉大輔、橋井則貴、新井康通、津元裕樹、川崎ナナ、広瀬信義、遠藤玉夫
  • Organizer: 第37回日本基礎老化学会大会
  • Place of Presentation: 大府
• [Presentation] Proteomics of Plasma Proteins in Japanese Semisuper Centenarians (2014)
  • Author(s): Miura, Y., Arai, Y., Toda, T., Hirose, N., Endo, T.
  • Organizer: 17th Biennial Meeting of Society for Free Radical Research International (SFRR 2014)
  • Place of Presentation: Kyoto
• [Presentation] Glycoproteomics study of plasma glycoproteins in Japanese semisuper centenarians (2013)
  • Author(s): Miura, Y., Itakura, Y., Arai, Y., Iwamoto, M., Toyoda, M., Kinoshita, H., Unuma, Y., Toda, T., Hirose, N., Endo, T.
  • Organizer: 12^<th> Human Proteome Organization (HUPO) World Congress
  • Place of Presentation: Yokohama
• [Presentation] 日本人超百寿者血漿のグライコプロテオミクス解析 (2013)
  • Author(s): 三浦ゆり、板倉陽子、豊田雅士、木下英樹、広瀬信義、遠藤玉夫
  • Organizer: 第32回日本糖質学会年会
  • Place of Presentation: 大阪
• [Presentation] Glycoproteomics study of plasma glycoproteins in Japanese semisuper centenarians (2013)
  • Author(s): Yuri Miura, Yoko Itakura, Yasumichi Arai, Machiko Iwamoto, Masashi Toyoda, Hideki Kinoshita, Yutaka Unuma, Tosifusa Toda, Nobuyoshi Hirose, Tamao Endo
  • Organizer: 12th Human Proteome Organization (HUPO) World Congress
  • Place of Presentation: Pacifico Yokohama (Kanagawa, Japan)
• [Presentation] レクチンマイクロアレイを用いた日本人超百寿者血漿のグライコプロテオミクス (2012)
  • Author(s): 三浦ゆり、板倉陽子、新井康通、豊田雅士、木下英樹、丸尾祐二、鵜沼豊、戸田年総、広瀬信義、遠藤玉夫
  • Organizer: 第35回日本基礎老化学会大会
  • Place of Presentation: 習志野
• [Presentation] レクチンマイクロアレイを用いた日本人超百寿者血漿のグライコプロテオミクス (2012)
  • Author(s): 三浦ゆり、板倉陽子、新井康通、豊田雅士、木下英樹、丸尾祐二、鵜沼 豊、戸田年総、広瀬信義、遠藤玉夫
  • Organizer: 第35回日本基礎老化学会大会
  • Place of Presentation: 東邦大学習志野キャンパス（千葉県）