Search for methods to control and treat allergy by regulating class switch recombination

• Project/Area Number: 24659147
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: Kyoto University
• Principal Investigator: SHIMIZU Akira 京都大学, 医学(系)研究科(研究院), 教授 (00162694)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 免疫学 / クラススイッチ / 遺伝子組換え / アレルギー / 免疫制御

Research Abstract

Focused on the regulation of IgE production and regulation of class switching to IgA, a trial to manipulate of the target gene specificity of class switch recombination (CSR) by intervening its place or regulatory factors was performed.
It was examined whether the specific CSR could be controlled in an in vitro culture by the factors like Id2 and Runx2/3 which are known to be involved in the regulation of CSR to IgE and IgA, respectively. It was also examined whether such factors could guide the cells to switch to IgA after the cells were induced to IgE CSR.
By the above experiments, a possibility was tested that intervening the place or regulatory factors of CSR to manipulate specificity of CSR and could be a target to control allergy by eliminating IgE and to strengthen first-line protection against infectious diseases by enhancing IgA production.

Research Products

• [Journal Article] An exploratory clinical study on the safety and efficacy of an autologous fibroblast-seeded artificial skin cultured with animal product-free medium in patients with diabetic foot ulcers (2014)
  • Author(s): Morimoto, N., Ito, T., Takemoto, S., Katakami, M., Kanda, N., Tada, H., Tanaka, S., Teramukai, T., Kawai, K., Nakamura, Y., Kasai, Y., Yokode, M., Maekawa, T., Shimizu, A. and Suzuki, S.
  • Journal Title: Internat. Wound Journal Volume: 11巻 Pages: 183-189
  • Peer Reviewed
• [Journal Article] Functional evaluation of activation-dependent alterations in the sialoglycan composition of T cells (2014)
  • Author(s): Naito-Matsui, Y., Takada, S., Kano, Y., Iyoda, T., Sugai, M., Shimizu, A., Inaba, K., Nitschke, L., Tsubata, T., Oka, S., Kozutsumi, Y. and Takematsu, H.
  • Journal Title: J. Biol. Chem. Volume: 289巻 Pages: 564-1579
  • Peer Reviewed
• [Journal Article] Functional evaluation of activation-dependent alterations in the sialoglycan composition of T cells. (2014)
  • Author(s): Naito-Matsui Y, Takada S, Kano Y, Iyoda T, Sugai M, Shimizu A, Inaba K, Nitschke L, Tsubata T, Oka S, Kozutsumi Y, and Takematsu H.
  • Journal Title: J. Biol. Chem. Volume: 289 Issue: 3 Pages: 1564-1579
  • DOI: 10.1074/jbc.m113.523753
  • Peer Reviewed / Open Access
• [Journal Article] An exploratory clinical study on the safety and efficacy of an autologous fibroblast-seeded artificial skin cultured with animal product-free medium in patients with diabetic foot ulcers (2014)
  • Author(s): Morimoto N, Ito T, Takemoto S, Katakami M, Kanda N, Tada H, Tanaka S, Teramukai S, Kawai K, Nakamura Y, Kasai Y, Yokode M, Maekawa T, Shimizu A, Suzuki S
  • Journal Title: International Wound Journal Volume: 11 Issue: 2 Pages: 183-189
  • DOI: 10.1111/j.1742-481x.2012.01064.x
  • Peer Reviewed
• [Journal Article] Evasion from accelerated blood clearance of nanocarrier named as "Lactosome" induced by excessive administration of Lactosome (2013)
  • Author(s): Hara, E., Makino, A., Kurihara, K., Sugai, M., Shimizu, A., Hara, I., Ozeki, E. and Kimura, S.
  • Journal Title: Biochem. Biophys. Acta-General Sub. Volume: 1830巻 Pages: 4046-4052
  • Peer Reviewed
• [Journal Article] Novel collagen/gelatin scaffold with sustained release of basic fibroblast growth factor : clinical trial for chronic skin ulcers (2013)
  • Author(s): Morimoto, N., Yoshimura, K., Niimi, M., Ito, T., Aya, R., Fujitaka, J., Tada, H., Teramukai, S., Murayama, T., Toyooka, C., Miura, K., Takemoto, S., Kanda, N., Kawai, K., Yokode, M., Shimizu, A. and Suzuki, S.
  • Journal Title: Tissue Enginer, Part A Volume: 19巻 Pages: 1931-1940
  • NAID: 120005312059
  • Peer Reviewed
• [Journal Article] Evasion from accelerated blood clearance of nanocarrier named as "Lactosome" induced by excessive administration of Lactosome. (2013)
  • Author(s): Hara, E., Makino, A., Kurihara, K., Sugai, M., Shimizu, A., Hara, I., Ozeki, E. and Kimura, S.
  • Journal Title: Biochem. Biophys. Acta-General Sub. Volume: 1830 Issue: 8 Pages: 4046-4052
  • DOI: 10.1016/j.bbagen.2013.03.024
  • Peer Reviewed
• [Journal Article] Novel collagen/gelatin scaffold with sustained release of basic fibroblast growth factor: clinical trial for chronic skin ulcers. (2013)
  • Author(s): Morimoto, N., Yoshimura, K., Niimi, M., Ito, T., Aya, R., Fujitaka, J., Tada, H., Teramukai, S., Murayama, T., Toyooka, C., Miura, K., Takemoto, S., Kanda, N., Kawai, K., Yokode, M., Shimizu, A. and Suzuki, S.
  • Journal Title: Tissue Engineer. Part A Volume: 19 Issue: 17-18 Pages: 1931-1940
  • DOI: 10.1089/ten.tea.2012.0634
  • NAID: 120005312059
  • Peer Reviewed
• [Journal Article] In situ differentiation of CD8aa T cells from CD4 T cells in peripheral lymphoid tissues (2012)
  • Author(s): Nambu, Y., Hayashi, T., Jang, K.J., Aoki, K., Mano, H., Nakano, K., Osato, M., Takahashi, K., Itoh, K., Teramukai, S., Komori, T., Fujita, J., Ito, Y., Shimizu, A. and Sugai, M.
  • Journal Title: Scientific Reports Volume: 2巻 Pages: 642-642
  • Peer Reviewed
• [Journal Article] In situ differentiation of CD8αα T cells from CD4 T cells in peripheral lymphoid tissues (2012)
  • Author(s): Nambu Y., Hayashi T., Jang KJ, Aoki K., Mano H., Nakano K., Osato M., Takabashi K., Itoh K., Teramukai S., Komon T., Fujita J., Ito Y., Shimizu
  • Journal Title: Scientific Reports Volume: 2 Issue: 1 Pages: 930-930
  • DOI: 10.1038/srep00642
  • Peer Reviewed