Extraction of high quality genomic DNA from postmortem tissue
| Project/Area Number |
24659159
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NOGUCHI Masayuki 筑波大学, 医学医療系, 教授 (00198582)
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| Research Collaborator |
NAKAGAWA Tomoki
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 病理解剖 / DNA / 死因究明 / 病理学 / 次世代シークエンス / バイオバンク / ゲノムDNA / 核酸 / 死後臓器組織 / FACS解析 / 人体病理学 |
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| Outline of Final Research Achievements |
Autopsy is the one of the most important method for elucidating the cause and mechanism of the disease. The problem is that nucleic acids in postmortem tissue are easy to degenerate momentarily, so it is very important that we know the extent of the degeneration of the nucleic acids in postmortem tissue.
In this research, we showed clearly that high molecular weight genomic DNA was preserved in the postmortem tissue freezed at －80℃, regardless of kinds of the organ and postmortem time. Furthermore, it was suggested these postmortem DNA could be applied to the next-generation sequencing analysis.
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Report
(4 results)
Research Products
(1 results)
