| Project/Area Number |
24659161
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | DNA 付加体 / 修飾核酸 / 分子疫学 / etheno DNA adduct / demethylation / TET / TDG / IDH / 塩基修復 / DNA付加体 / DNA修飾 / 発がん / 修復遺伝子 / 胃がん / 人体病理 |
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| Research Abstract |
Adductomics analysis of alkane substituted exocyclic etheno DNA adducts in human stomach mucosa having gastric cancer was performed for the cases from Lujiang County, Anhui, China, and from Hamamatsu, Japan. Discrimination analysis using quantitative value of 7 lipid per oxidation derived DNA adducts revealed that two regions showed distinctive adductome profile. 3 of 7 DNA adducts in this category were greater in Japanese gastric mucosa than Chinese gastric mucosa, which may suggest gastric carcinogenesis in Japan favor inflammatory or metabolic background. Furthermore, we quantitated 5'-hydroxymethyl deoxycytidine, and its derivatives carboxyl deoxycytidine, and formyl deoxycytidine in gastric cancer and adjacent non-tumor tissues and found the relationship between TET2 expression and 5hmdC. In this attempt we could not identify the adducts specifically high in endemic area of gastric cancer (Lujiang), thus further investigation is necessary.
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