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Development of diagnostic agent for dementia

Research Project

Project/Area Number 24659169
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Human pathology
Research InstitutionTokyo Metropolitan Geriatric Hospital and Institute of Gerontology

Principal Investigator

ISHIGAMI Akihito  地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究部長 (50270658)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords認知症 / 臨床検査試薬 / アルツハイマー病 / 老化 / シトルリン化タンパク質
Outline of Final Research Achievements

Peptidylarginine deiminases (PADs) are post-translational modification enzymes that citrullinate protein arginine residues. Abnormal protein citrullination by PAD2 has been closely associated with the pathogenesis of neurodegenerative disorders such as Alzheimer’s disease (AD). In this study, we tried to develop a novel method for sandwich enzyme-linked immunosorbent assay (ELISA) for the determination of citrullinated proteins which may become a useful marker of human neurodegenerative diseases.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (17 results)

All 2014 2013 2012 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (8 results) (of which Invited: 1 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Peptidylarginine deiminase and protein citrullination in prion diseases: Strong evidence of neurodegeneration.2013

    • Author(s)
      Jang, B., Ishigami, A., Maruyama, N., Carp, R.I., Kim, Y.S., Choi, E.K.
    • Journal Title

      Prion

      Volume: 7 Issue: 1 Pages: 42-46

    • DOI

      10.4161/pri.22380

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Two novel sandwich ELISAs identify PAD4 levels and PAD4 autoantibodies in patients with rheumatoid arthritis.2013

    • Author(s)
      Ishigami, A., Uchida, Y., Miyazaki, T., Handa, S., Choi, E.K,, Kim, Y.S,, Kasahara, Y., Maruyama, N.
    • Journal Title

      Mod Rheumatol.

      Volume: 23 Issue: 4 Pages: 794-803

    • DOI

      10.1007/s10165-012-0748-0

    • NAID

      10031189176

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Two novel sandwich ELISAs identify PAD4 levels and PAD4 autoantibodies in patients with rheumatoid arthritis.2012

    • Author(s)
      Ishigami et al.
    • Journal Title

      Mod Rheumatol.

      Volume: in press

    • NAID

      10031189176

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Peptidylarginine deiminase modulates the physiological roles of enolase via citrullination: links between altered multifunction of enolase and neurodegenerative diseases.2012

    • Author(s)
      Jang et al.
    • Journal Title

      Biochem. J.

      Volume: 445 Pages: 183-192

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] シトルリン化タンパク質特異的モノクローナル抗体の作製と特異性の解析2014

    • Author(s)
      加賀美弥生、丸山直記、石神昭人
    • Organizer
      第37回 日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒトグリオーマ細胞株U-251MG細胞でのPAD2誘導に伴うシトルリン化タンパク質の増加2014

    • Author(s)
      増冨裕文、下門顕太郎、石神昭人
    • Organizer
      第37回 日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒトグリオーマ細胞株U-251MG細胞におけるcAMPによるPAD2の誘導2014

    • Author(s)
      増冨裕文、下門顕太郎、丸山直記、石神昭人
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] PADI4測定系の確立と臨床応用2014

    • Author(s)
      石神昭人
    • Organizer
      第58回日本リウマチ学会総会・学術集会
    • Place of Presentation
      東京
    • Year and Date
      2014-04-24
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Age-related changes of peptidylarginine deiminase 2 in the mouse brain.2013

    • Author(s)
      Kagami, Y., Shimada, N., Handa, S., Choi, E.K., Kim, Y.S., Ito, H., Maruyama, N., Ishigami, A.
    • Organizer
      The 20th IAGG World Congress of Gerontology and Geriatrics (IAGG 2013)
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2013 Research-status Report
  • [Presentation] Peptidylarginine deiminase 2-mediat ed protein citrullinati on in prion disease.2013

    • Author(s)
      Kim, H.J., Jang, B., Jin, J.K., Jeon, Y.C., Ishigami, A., Maruyama, N., Carp, R.I., Kim, Y.S., Choi, E.K.
    • Organizer
      The 20th IAGG World Congress of Gerontology and Geriatrics (IAGG 2013)
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2013 Research-status Report
  • [Presentation] 腫瘍組織におけるシトルリン化タンパク質の局在と病態との相関.2012

    • Author(s)
      加賀美弥生 他
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] 関節リウマチの早期診断を目指した新規hPAD4測定法及びhPAD4自己抗体検出法の確立.2012

    • Author(s)
      半田節子 他
    • Organizer
      第85回日本生化学大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Book] Ishigami, A. Choi, E.K., Kim, Y.S., Maruyama, N.2014

    • Author(s)
      Deimination in Alzheimer’s Disease. In: Protein Deimination in Human Health and Disease.
    • Total Pages
      433
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] Deimination in Prion Diseases. In: Protein Deimination in Human Health and Disease.2014

    • Author(s)
      Choi, E.K., Jang, B., Ishigami, A., Maruyama, N., Carp, R.I.,Kim, Y.K.
    • Total Pages
      433
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Remarks] 分子老化制御

    • URL

      http://www.aging-regulation.jp

    • Related Report
      2014 Annual Research Report
  • [Remarks] 老化制御研究チーム 分子老化制御

    • Related Report
      2013 Research-status Report
  • [Remarks] http://www.aging-regulation.jp/

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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