| Project/Area Number |
24659179
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | エピジェネティクス / Dot1L / メチル化 / 可視化 / ヒストンメチル化 / FRET |
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| Outline of Final Research Achievements |
Methylation at lysine 79 in histone H3 (H3K79me) is known to contribute transcriptional activation, development of circular system, and differentiation of ES cells. However, no techniques to visualize H3K79me in live cells have been established yet. In this study, first, Visualization of association between a H3K79me binding protein and H3K79me by using FRET technique was challenged, but no significant results were obtained. Thus, I next searched as-yet-unknown proteins to recognize H3K79me, and identified several candidate proteins. This result will not only enables H3K79me to be visualized, but also contribute to uncover the physiological roles of H3K79me.
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