Selective isolation of pseudopodia by using excimer laser ablation

• Project/Area Number: 24659184
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Experimental pathology
• Research Institution: Kinki University
• Principal Investigator: INOUE Takao 近畿大学, 医学部, 助教 (00441006)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 癌の浸潤・転移 / プロテオミクス

Research Abstract

Pseudopodia (actin-rich protrusion) are considered to be important for cancer metastasis. Tumor cells were cultured on a porous membrane to form pseudopodia in membrane pores. An excimer laser, which was used for LASIK, was employed for removing cell body on a membrane to selectively isolate pseudopodia in membrane pores. The proteins extracted from pseudopodia were analyzed and identified. Of those proteins, alpha-Parvin, which highly expressed in pseudopodia, was characterized. The results showed that the protein was involved in pseudopodial length and density, and cell migration ability.

Research Products

• [Journal Article] α- Parvin, a pseudopodial constituent, promotes cell motility and is associated with lymph node metastasis of lobular breast carcinoma. (2014)
  • Author(s): Ito M, Hagiyama M, Mimae T, Inoue T, Kato T, Yoneshige A, Nakanishi J, Kondo T, Okada M, Ito A.
  • Journal Title: Breast Cancer Res Treat Volume: 144 Issue: 1 Pages: 59-69
  • DOI: 10.1007/s10549-014-2859-0
  • Peer Reviewed / Open Access
• [Journal Article] Increased ectodomain shedding of lung-epithelial cell adhesion molecule 1 as a cause of increased alveolar cell apoptosis in emphysema. (2014)
  • Author(s): Mimae T, et al
  • Journal Title: Thorax Volume: 69 Issue: 3 Pages: 223-231
  • DOI: 10.1136/thoraxjnl-2013-203867
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cell adhesion molecule 1 is a new osteoblastic cell adhesion molecule and a diagnostic marker for osteosarcoma. (2013)
  • Author(s): Inoue T, Hagiyama M, Enoki E, Sakurai MA, Tan A, Wakayama T, Iseki S, Murakami Y, Fukuda K, Hamanishi C, Ito A.
  • Journal Title: Life Sci Volume: 92 Issue: 1 Pages: 91-99
  • DOI: 10.1016/j.lfs.2012.10.021
  • Peer Reviewed
• [Journal Article] Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve-mast cell interaction in a hapten-induced mouse model of atopic dermatitis (2013)
  • Author(s): Man Hagiyama, Takao Inoue, Tadahide Furuno, Takanori Iino, Saori Itami, Mamoru Nakanishi, H. Asada, Yoichiroh Hosokawa and Akihiko Ito
  • Journal Title: Brit. J. Dermato Volume: (In press) Issue: 4 Pages: 771-778
  • DOI: 10.1111/bjd.12108
  • Peer Reviewed
• [Presentation] 新規骨芽細胞接着分子cell adhesion molecule 1は骨芽細胞分化過程において一過性に発現する (2012)
  • Author(s): 井上敬夫、萩山満、榎木英介、伊藤彰彦
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京