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Isolation of longevity related genes of prasitic nematodes with east epression system

Research Project

Project/Area Number 24659190
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Parasitology (including Sanitary zoology)
Research InstitutionUniversity of Miyazaki

Principal Investigator

MARUYAMA Haruhiko  宮崎大学, 医学部, 教授 (90229625)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords線虫 / 寄生 / 寿命 / ゲノム / インスリンシグナル / 寄生虫
Research Abstract

Parasitic animals live longer as compared with related free-living species. In order to elucidate molecular basis for the longevity, we constructed a high-quality draft genome of an intestinal nematode, Strongyloides venezuelensis, to approach this issue with bioinformatics. Statistics of our draft genome were as follows; total size 55.5 Mb, longest scaffold 15Mb, N50 scaffold length 2Mb, with 18,048 predicted coding genes.
One of the genes which exist only in parasitic nematodes but not in free-living nematodes was that for ferrochelatase, which catalyzes the final step of hem-biosynthesis. We confirmed that this gene of S. venezuelensis was perfectly functional, though all nematodes including animal parasitic nematodes do not synthesize hem by themselves. Phylogenetic analysis suggested that animal parasitic nematodes acquired this gene from bacteria through horizontal gene transfer. The physiological role could be to detoxify hem, which is highly toxic to cells.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (24 results)

All 2014 2013 2012 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (15 results)

  • [Journal Article] The role of B-cells in immunity against adult Strongyloides venezuelensis2013

    • Author(s)
      El-Malky MA, Maruyama H, Al-Harthi SA, El-Beshbishi SN, Ohta N.
    • Journal Title

      Parasit Vectors

      Volume: 6 Issue: 1 Pages: 148-148

    • DOI

      10.1186/1756-3305-6-148

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a Bacteria-Like Ferrochelatase in Strongyloides venezuelensis, an Animal Parasitic Nematode2013

    • Author(s)
      Nagayasu E, Ishikawa SA, Taketani S, Chakraborty G, Yoshida A, Inagaki Y, Maruyama H.
    • Journal Title

      PLOS ONE

      Volume: 8(3)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptomic analysis of four developmental stages of Strongyloides venezuelensis2013

    • Author(s)
      Nagayasu E, Ogura Y, Itoh T, Yoshida A, Chakraborty G, Hayashi T, Maruyama H.
    • Journal Title

      Parasitol Int

      Volume: 62(1) Pages: 57-65

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a bacteria-like ferrochelatase gene in Strongyloides venezuelensis, an animal parasitic nematode.2013

    • Author(s)
      Nagayasu E, Ishikawa S, Taketani S, Ooka T, Chakraborty G, Yoshida A, Inagaki Y, Hayashi T, Maruyama H.
    • Journal Title

      PLoS One

      Volume: 8 Issue: 3 Pages: 1-11

    • DOI

      10.1371/journal.pone.0058458

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Efficacy and safety of paromomycin for treating amebiasis in Japan2013

    • Author(s)
      Kikuchi T, Koga M, Shimizu S, Miura T, Maruyama H, Kimura M
    • Journal Title

      Parasitology International

      Volume: 62 Issue: 6 Pages: 497-501

    • DOI

      10.1016/j.parint.2013.07.004

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptomic analysis of four developmental stages of Strongyloides venezuelensis2013

    • Author(s)
      Nagayasu E, Ogura Y, Itoh T, Yoshida A, Chakraborty G, Hayashi T, Maruyama H
    • Journal Title

      Parasitology International

      Volume: 62(1) Issue: 1 Pages: 57-65

    • DOI

      10.1016/j.parint.2012.09.006

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] A novel C-type lectin identified by EST analysis in tissue migratory larvae of Ascaris suum2012

    • Author(s)
      Yoshida A, Nagayasu E, Horii Y, Maruyama H
    • Journal Title

      Parasitology Research

      Volume: 110 Issue: 4 Pages: 1583-6

    • DOI

      10.1007/s00436-011-2677-9

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Paragonimiasis in Japan : A Twelve-year Retrospective Case Review(2001–2012)

    • Author(s)
      Nagayasu E, Yoshida A, Hombu A, Horii Y, Maruyama H.
    • Journal Title

      Int. Med

      Volume: (in press)

    • NAID

      130004903022

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficient de novo assembly of highly heterozygous genomes from whole-genome shotgun short reads

    • Author(s)
      Kajitani R, Toshimoto K, Noguchi H, Toyoda A, Ogura Y, Okuno M, Yabana M, Harada M, Nagayasu E, Maruyama H, Hayashi T, Kohara Y, Fujiyama A, Itoh T.
    • Journal Title

      Genome. Res

      Volume: (in press)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 糞線虫感染幼虫の宿主感染にともなう発育再開メカニズムの探求2014

    • Author(s)
      長安英治、日野明紀菜、前田安信、丸山治彦
    • Organizer
      第83回日本寄生虫学会大会
    • Place of Presentation
      愛媛県松山市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 寄生虫症血清診断から見た肺吸虫症の最近の動向2013

    • Author(s)
      長安英治、吉田彩子、本部エミ、黒木美香、丸山治彦
    • Organizer
      第24回日本臨床寄生虫学会学術大会
    • Place of Presentation
      奈良市
    • Year and Date
      2013-06-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Genome and transcriptome analysis of Strongyloides venezuelensis, an animal parasitic nematode2013

    • Author(s)
      Nagayasu E, Ogura Y, Ito T, Yoshida I, Chakraborty G, Hayashi T, Maruyama H.
    • Organizer
      International Symposium on Genome Science"Expanding Frontiers of Genome Science
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Migratory pattern of Toxocara cati and Toxocara canis in pigs2013

    • Author(s)
      Yoshida A, Poulsen CS, Skallerup P, Maruyama H, Thamsborg SM, Nejsum P.
    • Organizer
      24th International Conference of the WAAVP
    • Place of Presentation
      Perth
    • Related Report
      2013 Final Research Report
  • [Presentation] Migratory pattern of Toxocara cati and Toxocara canis in pigs as a model for human infection2013

    • Author(s)
      Yoshida A, Skallerup P, Poulsen CS, Nejsum P, Maruyama H, Thamsborg SM.
    • Organizer
      Forum Cheju 16
    • Place of Presentation
      Seoul
    • Related Report
      2013 Final Research Report
  • [Presentation] Migratory pattern of Toxocara cati and Toxocara canis in pigs2013

    • Author(s)
      Poulsen CS, Yoshida A, Skallerup P, Maruyama H, Thamsborg SM, Nejsum P.
    • Organizer
      8th European Congress on Tropical Medicine and International Health
    • Place of Presentation
      Copenhagen
    • Related Report
      2013 Final Research Report
  • [Presentation] ベネズエラ糞線虫の動物宿主感染時における発育再開に関する研究2013

    • Author(s)
      長安英治、丸山治彦
    • Organizer
      第21回分子寄生虫ワークショップ
    • Place of Presentation
      兵庫県神戸市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 糞線虫の発育再開における栄養シグナル伝達の役割2013

    • Author(s)
      長安英治、丸山治彦
    • Organizer
      第11回分子寄生虫・マラリア研究フォーラム
    • Place of Presentation
      長崎県長崎市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Genome and transcriptome analysis of Strongyloides venezuelensis, an animal parasitic nematode.2013

    • Author(s)
      E. Nagayasu, Y. Ogura, T. Ito, I. Yoshida, G. Chakraborty, T. Hayashi, H. Maruyama.
    • Organizer
      International Symposium on Genome Science “Expanding Frontiers of Genome Science.
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] ゲノム概要配列が未知の寄生虫研究における次世代型シーケンサの活用法2012

    • Author(s)
      長安英治、小椋義俊、伊藤武彦、吉田彩子、林哲也、丸山治彦
    • Organizer
      第10回日本寄生虫学会東日本支部会、第10回分子寄生虫マラリアフォーラム合同大会
    • Place of Presentation
      前橋市
    • Related Report
      2013 Final Research Report
  • [Presentation] ゲノム/トランスクリプトーム情報に基づく動物寄生関連遺伝子の検索2012

    • Author(s)
      長安英治、丸山治彦
    • Organizer
      第10回日本寄生虫学会東日本支部会、第10回分子寄生虫マラリアフォーラム合同大会
    • Place of Presentation
      前橋市
    • Related Report
      2013 Final Research Report
  • [Presentation] Serological diagnosis of Ascarid Visceral Larva Migrans with recombinant antigens2012

    • Author(s)
      Yoshida A, Nejsum P, Skallerup P, Thamsborg SM, Maruyama H.
    • Organizer
      ESCCAP Toxocara 2012
    • Place of Presentation
      Budapest
    • Related Report
      2013 Final Research Report
  • [Presentation] Migratory pattern of Toxocara cati and Toxocara canis in pigs2012

    • Author(s)
      Yoshida A, Poulsen CS, Skallerup P, Maruyama H, Thamsborg SM, Nejsum P.
    • Organizer
      ESCCAP Toxocara 2012
    • Place of Presentation
      Budapest
    • Related Report
      2013 Final Research Report
  • [Presentation] Bacteria-like ferrochelatase in animal parasitic nematodes2012

    • Author(s)
      Nagayasu E, Ishikawa S, Taketani S, Chakraborty G, Yoshida A, Inagaki, Y, Maruyama H.
    • Organizer
      The 11th Awaji International Forum on Infection and Immunity
    • Place of Presentation
      Awaji, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Bacteria-like ferrochelatase in animal parasitic nematodes.2012

    • Author(s)
      E. Nagayasu, S. Ishikawa, S. Taketani, G. Chakraborty, A. Yoshida, Y. Inagaki, H. Maruyama
    • Organizer
      The 11th Awaji International Forum on Infection and Immunity.
    • Place of Presentation
      Awaji, Japan
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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