• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

アメーバ共生細菌プロトクラミジアアンキリンエフェクターの機能解析

Research Project

Project/Area Number 24659194
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Bacteriology (including Mycology)
Research InstitutionHokkaido University

Principal Investigator

山口 博之  北海道大学, 大学院保健科学研究院, 教授 (40221650)

Co-Investigator(Kenkyū-buntansha) 中村 眞二  順天堂大学, 医学(系)研究科(研究院), 助教 (40207882)
松尾 淳司  北海道大学, 大学院保健科学研究院, 助教 (50359486)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords微生物間共生 / 原始クラミジア / エフェクター / クラミジア / アンキリンリピート / IV型分泌装置 / アメーバ / 共生細菌
Research Abstract

以下の2点についてこれ迄に明らかにした。
1. 札幌の河川水より分離・株化したアメーバに共生する偏性細胞内寄生性細菌Protochlamydia R18株のドラフトゲノムを決定し、比較ゲノム解析より蛋白蛋白相互作用に深く関わるアンキリン(Ank)モチーフやロイシンリッチリピート(LRR)を持つ分子をコードする計38つの遺伝子を同定した。
2. 同定したAnkやLRRを持つ分子をコードする共生細菌の遺伝子がアメーバ内で実際に発現していることをRT-PCRにて確認した。現在、Protochlamydia R18ドラフトゲノム情報を基にカスタマイズしたDNAマイクロアレイを用いてアメーバ内での共生細菌の遺伝子発現変化の網羅的な解析を進めている。

Report

(2 results)
  • 2013 Annual Research Report
  • 2012 Research-status Report
  • Research Products

    (17 results)

All 2013 2012 2011

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (11 results) (of which Invited: 2 results)

  • [Journal Article] Protochlamydia Induces Apoptosis of Human HEp-2 Cells through Mitochondrial Dysfunction Mediated by Chlamydial Protease-Like Activity Factor.2013

    • Author(s)
      Junji Matsuo, Shinji Nakamura, Atsushi Ito, Tomohiro Yamazaki, Kasumi Ishida. Yasuhiro Hayashi, Mitsutaka Yoshida, Kaori Takahashi, Tsuyoshi Sekizuka, Fumihiko Takeuchi, Makoto Kuroda, Hiroki Nagai, Kyoko Hayashida, Chihiro Sugimoto. Hiroyuki Yamaguchi
    • Journal Title

      PLoS One

      Volume: 8 Issue: 2 Pages: e56005-e56005

    • DOI

      10.1371/journal.pone.0056005

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Chlamydophila pneumoniae in Human Immortal Jurkat cells and Primary Lymphocytes Uncontrolled by Interferon-γ2013

    • Author(s)
      Kasumi Ishida. Takeru Kubo, Ayumi Saeki, Chikayo Yamane, Junji Matsuo, Yimin, Shinji Nakamura, Yasuhiro Hayashi, Miyuki Kunichika, Mitsutaka Yoshida, Kaori Takahashi, Itaru Hirai, Yoshimasa Yamamoto, Kenichiro Shibata, Hiroyuki Yamaguchi
    • Journal Title

      Microbes and Infection

      Volume: 15 Issue: 3 Pages: 192-200

    • DOI

      10.1016/j.micinf.2012.11.006

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of steroid receptor antagonist RU486 (mifepristone) on Chlamydophila pneumoniae in IFN g-induced persistent infection model with epithelial HEp-2 cells2012

    • Author(s)
      Ishida K
    • Journal Title

      Journal of Infection and Chemothrapy

      Volume: 18 Issue: 1 Pages: 22-29

    • DOI

      10.1007/s10156-011-0274-6

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ciliates expel environmental Legionella-laden pellets to stockpile food.2012

    • Author(s)
      Fuhito Hojo, Daisuke Sato, Junji Matsuo, Masaki Miyake, Shinji Nakamura, Miyuki Kunichika, Yasuhiro Hayashi, Mitsutaka Yoshida, Kaori Takahashi, Hiromu Takemura, Shigeru Kamiya and Hiroyuki Yamaguchi
    • Journal Title

      Appl. Environ. Microbiol.

      Volume: 78 Issue: 15 Pages: 5247-5257

    • DOI

      10.1128/aem.00421-12

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Chlamydia trachomatis serovar L2 infection model using human lymphoid Jurkat cells2012

    • Author(s)
      Kubo T, Ishida K, Matsuo J, Nakamura S, Hayashi Y, Sakai H, Yoshida M, Takahashi K, Hirai I, Yamamoto Y, Yamaguchi H
    • Journal Title

      Microb Pathog

      Volume: 53 Pages: 1-11

    • NAID

      120005946711

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Frequency of Chlamydia trachomtis in Ureaplasma-positive healthy women attending their first prenatal visit in a community hospital in Sapporo, Japan2011

    • Author(s)
      Yamazaki T
    • Journal Title

      BMC Infectious Diseases

      Volume: (in press) Issue: 1 Pages: 1-8

    • DOI

      10.1186/1471-2334-12-82

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Ureaplasma parvumの存在はIFN-γ によるChlamydia trachomatis発育抑制効果を減弱させる2013

    • Author(s)
      山崎智拡 (山口博之)
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      幕張メッセ (千葉市)
    • Related Report
      2012 Research-status Report
  • [Presentation] アメーバ共生細菌原始クラミジアは低温下にてヒト上皮細胞株HEp-2細胞内で増殖する2013

    • Author(s)
      山根千夏世 (山口博之)
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      幕張メッセ (千葉市)
    • Related Report
      2012 Research-status Report
  • [Presentation] アメーバ共生難培養性細菌原始クラミジアの比較ゲノム解析2013

    • Author(s)
      山口博之 (山口博之)
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      幕張メッセ (千葉市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 原始クラミジアProtochlamydiaはchlamydial protease-like activity factor (CPAF) を介してヒト上皮系細 胞株HEp-2 にアポトーシスを誘導する2013

    • Author(s)
      松尾淳司 (山口博之)
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      幕張メッセ (千葉市)
    • Related Report
      2012 Research-status Report
  • [Presentation] クラミジア感染ヒトリンパ球細胞株Jurkat細胞内における遺伝子変化2013

    • Author(s)
      石田香澄 (山口博之)
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      幕張メッセ (千葉市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 原始クラミジア, それは病原性クラミジアの細胞内生存戦略を紐解くヒントである2012

    • Author(s)
      山口博之
    • Organizer
      第30回日本クラミジア研究会
    • Place of Presentation
      国立感染症研究所(東京都)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Amoebal endosymbiont primitive chlamydiae: a hint to uncover the intracellular survival strategy of human pathogenic chlamydiae2012

    • Author(s)
      Hiroyuki Yamaguchi
    • Organizer
      The 11th Korea-Japan International Symposium on Microbiology
    • Place of Presentation
      ロッテリゾートホテル (Korea)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Parachlamydia acanthamoebae attachment to amoebae is required for several amoebal secreted molecules and serine-protease activity2012

    • Author(s)
      Hayashi Yasuhiro (Yamaguchi Hiroyuki)
    • Organizer
      ASM 112th General Metting
    • Place of Presentation
      Moscone Center (USA)
    • Related Report
      2012 Research-status Report
  • [Presentation] Pathogenic Chlamydial Growth in Human Immortal Jurkat Lymphoid Cells Uncontrolled by Interferon-γ2012

    • Author(s)
      Kasumi Ishida (Hiroyuki Yamaguchi)
    • Organizer
      ASM 112th General Metting
    • Place of Presentation
      Moscone Center (USA)
    • Related Report
      2012 Research-status Report
  • [Presentation] Prevalence of Chlamydia trachomatis and Ureaplasma species in healthy women2012

    • Author(s)
      Tomohiro Yamazaki (Hiroyuki Yamaguchi)
    • Organizer
      ASM 112th General Metting
    • Place of Presentation
      Moscone Center (USA)
    • Related Report
      2012 Research-status Report
  • [Presentation] Environmental chlamydia, Protochlamydia induces caspase-dependent apoptosis to human immortal HEp-2 cells by triggering a mitochondrial pathway2012

    • Author(s)
      Junji Matsuo (Hiroyuki Yamaguchi)
    • Organizer
      ASM 112th General Metting
    • Place of Presentation
      Moscone Center (USA)
    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi