Comprehensive analysis on dengue viral quasispecies in human and mosquito body.

• Project/Area Number: 24659210
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Nagasaki University
• Principal Investigator: HASEBE Futoshi 長崎大学, 国際連携研究戦略本部, 教授 (20253693)
• Co-Investigator(Renkei-kenkyūsha): MORITA Kouichi 長崎大学, 熱帯医学研究所, 教授 (40182240) ; NABESHIMA Takeshi 長崎大学, 熱帯医学研究所, 助教 (30546859)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: デングウイルス / ウイルス準種 / 次世代シーケンサー / 細胞向性 / 多様性 / 病原性 / ベトナム

Outline of Final Research Achievements

Comprehensive dengue viral gene analysis was conducted on DENV-1 and -2 infected patient serum samples by using second generation sequencer. Preliminary analysis showed that the 3’ terminal codon of NS5 gene was changed and there was an addition of 27 amino acids resulting in the extension at the 3’ end of NS5 protein of the DENV-2 virus from some patient samples. DENV-1 to -4 strains were isolated by using C6/36 and Vero cell lines and the entire sequences were analyzed by second generation sequencer. Among the DENV-1 isolates, we confirmed cell-line dependent amino acid sequence which is related in cellular tropism to either mosquito or mammals.

Research Products

• [Journal Article] A dengue virus serotype 4-dominated outbreak in central Vietnam, 2013. (2015)
  • Author(s): Takamatsu Y, Nabeshima T, Nguyen Thi Thu Thuy, Dang Thi Dinh, Pham Hoai Linh Ly, Pham Thi Hang, Nguyen Thi Nam Lien, Phu Ly Minh Huong, Bui Thu Thuy, Le Thi Quynh Mai, Morita K, Hasebe F.
  • Journal Title: Journal of Clinical Virology Volume: 66 Pages: 24-26
  • NAID: 120006987102
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 蚊媒介性ウイルス感染－デング熱・日本脳炎・チクングニア熱 (2015)
  • Author(s): 長谷部 太
  • Journal Title: 科学療法の領域 2015年5月号（2015年4月25日発行）特集「野外活動と感染症」 Volume: 5月号 Pages: 57-65
• [Journal Article] デング熱、日本脳炎、ウエストナイル熱 (2014)
  • Author(s): 長谷部太
  • Journal Title: 臨床と微生物 話題の新興・再興感染症 Volume: 41 Pages: 309-315
• [Journal Article] Field trial on a novel control method for the dengue vector, Aedes aegypti by the systematic use of Olyset Net and pyriproxyfen in Southern Vietnam. (2013)
  • Author(s): Tsunoda T, Kawada H, Huynh TT, Luu ll, Le SH, Tran HN, Vu HT, Le HM, Hasebe F, Tsuzuki A, Takagi M.
  • Journal Title: Parasit Vectors Volume: 6 Issue: 1 Pages: 1-6
  • DOI: 10.1186/1756-3305-6-6
  • Peer Reviewed
• [Presentation] デングウイルスとデング熱 (2015)
  • Author(s): 長谷部 太
  • Organizer: 平成26年度新型インフルエンザ等新興・再興感染症研究推進事業シンポジウムプログラム「感染症は一国の問題ではない ～エボラ出血熱、デング熱を例として～」
  • Place of Presentation: ホテル椿山荘東京 ジュピターホール（東京都文京区）
  • Year and Date: 2015-02-16
  • Invited
• [Presentation] Dengue Situation in Vietnam and Quasispecies of Dengue Viruses. (2015)
  • Author(s): Futoshi Hasebe, Nguyen Thi Thu Thuy, Nguyen Co Thach, Dang Thi Dinh, Pham Thi Hang, Takeshi Nabeshima, Le Thi Quynh Mai, and Kouichi Morita.
  • Organizer: The Phase-End Seminar for the “Collaborative Study on Emerging and Re-emerging Infectious Diseases in Vietnam: Enhancement of Research Capacity Phase II, 2011-2015” project.
  • Place of Presentation: Hanoi Intercontinental Hotel, Vietnam
  • Year and Date: 2015-01-12
• [Presentation] Study of Unknown Acute Encephalitis in Vietnam. (2015)
  • Author(s): Futoshi Hasebe, Nguyen Thi Thu Thuy, Nguyen Co Thach, Dang Thi Dinh, Pham Thi Hang, Takeshi Nabeshima, Le Thi Quynh Mai, and Kouichi Morita.
  • Organizer: The Phase-End Seminar for the “Collaborative Study on Emerging and Re-emerging Infectious Diseases in Vietnam: Enhancement of Research Capacity Phase II, 2011-2015” project.
  • Place of Presentation: Hanoi Intercontinental Hotel, Vietnam
  • Year and Date: 2015-01-12
• [Presentation] デング熱/デング出血熱とは (2014)
  • Author(s): 長谷部 太
  • Organizer: 「緊急開催!!デング熱の脅威」 大下財団主催
  • Place of Presentation: ANAクラウンプラザホテル広島（広島市）
  • Year and Date: 2014-11-13
  • Invited
• [Presentation] Changing Pattern of DENV Serotypes in Vietnam. (2014)
  • Author(s): Nguyen Thi Thu Thuy, Takeshi Nabeshima, Guillermo Posadas-Herrera, Dang Thi Dinh, Maria Terrese G. Alonzo, Lady-Anne C. Suarez, Mya Myat Ngwe Tun, Nguyen Le Khanh Hang, Pham Hoai Linh Ly, Le Thi Quyenh Mai, Corazon C. Buerano, Filipinas F. Natividad, Futoshi Hasebe and Kouichi Morita.
  • Organizer: Asian-African Research Forum on Emerging and Reemerging Infections 2014
  • Place of Presentation: 仙台国際センター(宮城県仙台市青葉区）
• [Presentation] Molecular epidemiological study on dengue viruses in Vietnam.
  • Author(s): Nguyen Thi Thu Thuy, Dang Anh Tuan, Vu Dinh Luan, Truong Thi Ha, Takeshi Nabeshima, Posadas-Herrera Guillermo, Dang Thi Dinh, Pham Hoai Linh Ly, Nguyen Bao Ngoc, Le Thi Quynh Mai, Vu Thi Que Huong, Kouichi Morita and Futoshi Hasebe.
  • Organizer: Internal Seminar between Natinal Institute of Hygiene and Epidemiology and Nagasaki University
  • Place of Presentation: Novotel Ha Long Bay Hotel（ベトナム、クアンニン省、ハロン市）
• [Book] 感染症は一国の問題ではない。～エボラ出血熱、デング熱を例として～ (2015)
  • Author(s): 西條政幸、高田礼人、長谷部太、澤部京子
  • Total Pages: 20
  • Publisher: 公益社団法人国際校正事業団