| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
HIV-1 Gag protein was expressed in HeLa cells and influenza virus HA in MDCK cells. When the protein degradation kinetics were analyzed by Western blotting, we found the t1/2 of Gag 8-9 hrs and that of HA slightly shorter. Gag and HA were more rapidly degradated by the proteasome pathway, when ubiquitin was added to their N-termini. Based on the N-end rule, when the N-terminal methionine of Gag after ubquitin cleavage was substituted with arginine, the Gag was more efficiently degradated by the ubquitin/proteasome pathway. In contrast, the addition of LAMP1 to Gag altered the major degradation pathway for Gag from the proteasome pathway to the lysosomal pathway.
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