Identification of retrovirus-like DNA fragments in an AIDS patient

• Project/Area Number: 24659212
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: KATANO Harutaka 国立感染症研究所, その他部局等, その他 (70321867)
• Co-Investigator(Renkei-kenkyūsha): KURODA Makoto 国立感染症研究所, 病原体ゲノム解析研究センター, センター長 (80317411) ; HASEGAWA Hideki 国立感染症研究所, 感染病理部, 部長 (30301790)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ウイルス

Outline of Final Research Achievements

Genome fragments of unidentified retrovirus were discovered from the spleen tissue of an AIDS autopsy using a next generation sequencer. The whole genome of the retrovirus was 8kb of length containing gag, pol, env and protease genes, and belonged to beta-retrovirus family. A real-time PCR assay revealed that any genome of the virus was not detected in other human pathological samples, suggesting that the virus is rare and uncommon among human.

Research Products

• [Journal Article] Detection of trichodysplasia spinulosa-associated polyomavirus in a fatal case of myocarditis in a seven-month-old girl. (2014)
  • Author(s): Tsuzuki S, Fukumoto H, Mine S, Sato N, Mochizuki M, Hasegawa H, Sekizuka T, Kuroda M, Matsushita T, Katano H
  • Journal Title: Int J Clin Exp Pathol Volume: 7 Pages: 5308-5312
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Classification of AIDS-related lymphoma cases between 1987 and 2012 in Japan based on the WHO classification of lymphomas, fourth edition. (2014)
  • Author(s): Ota Y, Hishima T, Mochizuki M, Kodama Y, Moritani S, Oyaizu N, Mine S, Ajisawa A, Tanuma J, Uehira T, Hagiwara S, Yajima K, Koizumi Y, Shirasaka T, Kojima Y, Nagai H, Yokomaku Y, Shiozawa Y, Koibuchi T, Iwamoto A, Oka S, Hasegawa H, Okada S, Katano H
  • Journal Title: Cancer Med Volume: 3 Issue: 1 Pages: 143-153
  • DOI: 10.1002/cam4.178
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The prevalence of opportunistic infections and malignancies in autopsied patients with human immunodeficiency virus infection in Japan (2014)
  • Author(s): Katano H, Hishima T, Mochizuki M, Kodama Y, Oyaizu N, Ota Y, Mine S, Igari T, Ajisawa A, Teruya K, Tanuma J, Kikuchi Y, Uehira T, Shirasaka T, Koibuchi T, Iwamoto A, Oka S, Hasegawa H, Okada S, Yasuoka A
  • Journal Title: BMC Infect Dis Volume: 14 Issue: 1 Pages: 229-229
  • DOI: 10.1186/1471-2334-14-229
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Frequent detection of Merkel cell polyomavirus DNA in sera of HIV-1-positive patients (2013)
  • Author(s): Fukumoto H, Sato Y, Hasegawa H, Katano H
  • Journal Title: Virol J Volume: 10 Issue: 1 Pages: 84-84
  • DOI: 10.1186/1743-422x-10-84
  • Peer Reviewed
• [Journal Article] Pathogenic characterization of a cervical lymph node derived from a patient with Kawasaki disease. (2012)
  • Author(s): Katano H, Sato S, Sekizuka T, Kinumaki A, Fukumoto H, Sato Y, Hasegawa H, Morikawa S, Saijo M, Mizutani T, Kuroda M
  • Journal Title: Int J Clin Exp Pathol Volume: 5 Pages: 814-823
  • Peer Reviewed