• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Visualization of osteoblast impairments during bone marrow GVHD

Research Project

Project/Area Number 24659216
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionThe University of Tokyo

Principal Investigator

MATSUSHIMA Kouji  東京大学, 大学院医学系研究科, 教授 (50222427)

Co-Investigator(Kenkyū-buntansha) 上羽 悟史  東京大学, 医学(系)研究科(研究院), 講師 (00447385)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords移植・再生医療 / 免疫学 / 細胞・組織 / 病理学 / 癌
Research Abstract

We aimed to reveal the cellular and molecular mechanisms of the osteoblast impairments during bone marrow GVHD. In a minor mismatched mouse GVHD model, ALP+ osteoblasts were lost in the early phase, whereas hyperostosis and fibrosis in the marrow occured in late phase. Ex vivo co-culture experiments demonstrated that donor CD4+ T cells induced apoptosis in osteoblastic cell line MC3T3 and mesenchymal stem cell C3H10. In addition, donor CD4+ T cells also suppressed cell cycle and BMP dependent maturation of MC3T3 but not C3H10. Further studies on the cellular and molecular mechanisms of donor CD4+ T cell-induced impairments in bone marrow mesenchymal cells would lead to the development of strategies to overcome immuno-deficiency after allo-HSCT.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (12 results)

All 2014 2013 2012 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (8 results) (of which Invited: 4 results) Remarks (1 results)

  • [Journal Article] Bone Marrow Graft-versus-Host Disease: Evaluation of Its Clinical Impact on Disrupted Hematopoiesis after Allogeneic Hematopoietic Stem Cell Transplantation2014

    • Author(s)
      Souichi Shiratori, Mizuha Kosugi-Kanaya, Satoshi Ueha, Junichi Sugita1, Akio Shigematsu, Takeshi Kondo, Daigo Hashimoto, Katsuya Fujimoto, Tomoyuki Endo, Mitsufumi Nishio, Satoshi Hashino, Yoshihiro Matsuno, Kouji Matsushima, Junji Tanaka, Masahiro Imamura, Takanori Teshima
    • Journal Title

      Biol Blood Marrow Transplant

      Volume: 20 Issue: 4 Pages: 495-500

    • DOI

      10.1016/j.bbmt.2013.12.568

    • NAID

      120005427744

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Coordinated Changes in DNA Methylation in Antigen-Specific Memory CD4 T Cells2013

    • Author(s)
      Hashimoto S, Ogoshi K, Sasaki A, Abe J, Qu W, Nakatani Y, Ahsan B, Oshima K, Shand FH, Ametani A, Suzuki Y, Kaneko S, Wada T, Hattori M, Sugano S,Morishita S, Matsushima K
    • Journal Title

      J Immunol

      Volume: 190 Issue: 8 Pages: 4076-4091

    • DOI

      10.4049/jimmunol.1202267

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Stress-induced production of chemokines by hair follicles regulates the trafficking of dendritic cells in skin.2012

    • Author(s)
      Keisuke Nagao
    • Journal Title

      Nature Immunology

      Volume: 13(8) Issue: 8 Pages: 744-752

    • DOI

      10.1038/ni.2353

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Delayed and aberrant immunological reconstitution in allogeninc hematopoietic stem cell transplantation2013

    • Author(s)
      Kouji Matsushima
    • Organizer
      11th World Congress on Inflammation
    • Place of Presentation
      Natal(Brazil)
    • Year and Date
      2013-09-21
    • Related Report
      2013 Final Research Report
  • [Presentation] 骨髄GVHDにおける間葉系細胞障害の細胞・分子機序2013

    • Author(s)
      上羽 悟史
    • Organizer
      第34回日本炎症・再生医学会
    • Place of Presentation
      国立京都国際会館(京都)
    • Year and Date
      2013-07-02
    • Related Report
      2013 Final Research Report
  • [Presentation] 骨髄GVHDにおける間葉系細胞障害の細胞・分子機序2013

    • Author(s)
      上羽悟史、小杉瑞葉、王泳、横山顕大、朝比奈晏那、松島綱治
    • Organizer
      第34回日本炎症・再生医学会
    • Place of Presentation
      国立京都国際会館、京都
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Delayed and aberrant immunological reconstitution in allogeninc hematopoietic stem cell transplantation2013

    • Author(s)
      Kouji Matsushima
    • Organizer
      11th World Congress on Inflammation
    • Place of Presentation
      Natal, Brazil
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Bone marrow dysfunction and defective B lymphopoiesis during chronic GVHD2012

    • Author(s)
      小杉 瑞葉
    • Organizer
      第41回日本免疫学会
    • Place of Presentation
      神戸国際展示場(兵庫)
    • Year and Date
      2012-12-05
    • Related Report
      2013 Final Research Report
  • [Presentation] Destruction of hematopoietic niche after allogenic hematopoietic stem cell transplantation2012

    • Author(s)
      小杉 瑞葉
    • Organizer
      第34回日本炎症再生医学会
    • Place of Presentation
      ホテル日航福岡(福岡)
    • Year and Date
      2012-07-05
    • Related Report
      2013 Final Research Report
  • [Presentation] Destruction of hematopoietic niche after allogenic hematopoietic stem cell transplantation2012

    • Author(s)
      Mizuha Kosugi
    • Organizer
      The 34th Annual Meeting on the Japanese Society of Inflammation and Regeneration
    • Place of Presentation
      Fukuoka
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Bone marrow dysfunction and defective B lymphopoiesis during chronic GVHD2012

    • Author(s)
      Mizuha Kosugi
    • Organizer
      The 41st Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      Kobe
    • Related Report
      2012 Research-status Report
    • Invited
  • [Remarks] 東京大学 分子予防医学教室ホームページ

    • URL

      http://www.prevent.m.u-tokyo.ac.jp/

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi