Visualization of osteoblast impairments during bone marrow GVHD
| Project/Area Number |
24659216
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
上羽 悟史 東京大学, 医学(系)研究科(研究院), 講師 (00447385)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 移植・再生医療 / 免疫学 / 細胞・組織 / 病理学 / 癌 |
|---|
| Research Abstract |
We aimed to reveal the cellular and molecular mechanisms of the osteoblast impairments during bone marrow GVHD. In a minor mismatched mouse GVHD model, ALP+ osteoblasts were lost in the early phase, whereas hyperostosis and fibrosis in the marrow occured in late phase. Ex vivo co-culture experiments demonstrated that donor CD4+ T cells induced apoptosis in osteoblastic cell line MC3T3 and mesenchymal stem cell C3H10. In addition, donor CD4+ T cells also suppressed cell cycle and BMP dependent maturation of MC3T3 but not C3H10. Further studies on the cellular and molecular mechanisms of donor CD4+ T cell-induced impairments in bone marrow mesenchymal cells would lead to the development of strategies to overcome immuno-deficiency after allo-HSCT.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Bone Marrow Graft-versus-Host Disease: Evaluation of Its Clinical Impact on Disrupted Hematopoiesis after Allogeneic Hematopoietic Stem Cell Transplantation2014
Author(s)
Souichi Shiratori, Mizuha Kosugi-Kanaya, Satoshi Ueha, Junichi Sugita1, Akio Shigematsu, Takeshi Kondo, Daigo Hashimoto, Katsuya Fujimoto, Tomoyuki Endo, Mitsufumi Nishio, Satoshi Hashino, Yoshihiro Matsuno, Kouji Matsushima, Junji Tanaka, Masahiro Imamura, Takanori Teshima
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Journal Title
Biol Blood Marrow Transplant
Volume: 20
Issue: 4
Pages: 495-500
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Coordinated Changes in DNA Methylation in Antigen-Specific Memory CD4 T Cells2013
Author(s)
Hashimoto S, Ogoshi K, Sasaki A, Abe J, Qu W, Nakatani Y, Ahsan B, Oshima K, Shand FH, Ametani A, Suzuki Y, Kaneko S, Wada T, Hattori M, Sugano S,Morishita S, Matsushima K
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Journal Title
J Immunol
Volume: 190
Issue: 8
Pages: 4076-4091
DOI
Related Report
Peer Reviewed
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