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Origin and evolution of non-self discrimination in adaptive immunirty

Research Project

Project/Area Number 24659218
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Immunology
Research InstitutionThe University of Tokyo

Principal Investigator

NAGAWA Fumikiyo  東京大学, 理学(系)研究科(研究院), 講師 (10241233)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Yoshimasa  国立感染症研究所, 免疫部, 室長 (60311403)
Research Collaborator OSHIMA Kenshuro  東京大学, 大学院新領域創成科学研究科, 特任准教授 (40537411)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords獲得免疫系 / 無顎類 / 抗原受容体 / VLR / 遺伝子再編成 / 進化
Outline of Final Research Achievements

The jawless vertebrates (lampreys and hagfish), the most phylogenetically distant vertebrates from mammals, possess an alternative form of adaptive immune system that is mediated by antigen receptors called variable lymphocyte receptors (VLRs). A functional VLR gene, which consists almost entirely of several leucine-rich repeats (LLRs), is generated by the assembly of variable germline LRR gene segments that encode LRRs. Stepwise assembly of the gene segments occurs by replacement of the intervening non-coding DNA between the 5’ and 3’ constant regions by a process involving “copy choice”. Here, we have cloned and analyzed many partially assembled hagfish VLR genes. We found that they contained partially assembled structures at the 5’ and 3’ ends. These results suggest that a model where the assembly starts at both the 5’ and 3’ ends, and proceeds up to the middle LRRs, and the two ends are joined using a short homology between the LRR sequences.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (7 results)

All 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (6 results) (of which Invited: 1 results)

  • [Journal Article] Evolution of adaptive immunity: Implication of a third lymphocyte lineage in lampreys.2014

    • Author(s)
      Kishishita N and Nagawa F
    • Journal Title

      BioEssays

      Volume: 36 Issue: 3 Pages: 244-250

    • DOI

      10.1002/bies.201300145

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Antigen receptor gene assembly in hagfish2014

    • Author(s)
      Nagawa, F.
    • Organizer
      日本免疫学会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Year and Date
      2014-12-10 – 2014-12-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Antigen receptor gene assembly in hagfish2014

    • Author(s)
      Nagawa, F.
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Antigen receptor gene assembly in hagfish2014

    • Author(s)
      Nagawa, F.
    • Organizer
      11th International Congress on the Biology of Fish
    • Place of Presentation
      Edinburgh, the United Kingdom
    • Year and Date
      2014-08-03 – 2014-08-07
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Antigen-receptor Gene Assembly in Hagfish2014

    • Author(s)
      Fumikiyo Nagawa
    • Organizer
      International Congress on the Biology of Fish 2014
    • Place of Presentation
      エディンバラコンファレンスセンター(英国)
    • Related Report
      2013 Research-status Report
  • [Presentation] Antigen-receptor Gene Assembly in Hagfish2013

    • Author(s)
      名川文清
    • Organizer
      日本免疫学会
    • Place of Presentation
      幕張メッセ(千葉県)
    • Related Report
      2013 Research-status Report
  • [Presentation] Antigen-receptor Gene Assembly in Hagfish2013

    • Author(s)
      名川文清
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸コンベンションセンター(兵庫県)
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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