Identification of multi-level regulation of gene expression that determines B cell development

• Project/Area Number: 24659220
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Osaka University
• Principal Investigator: BABA Yoshihiro 大阪大学, 免疫学フロンティア研究センター, 准教授 (20415269)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: B細胞分化 / クロマチン動的構造変化 / H3ヒストンバリアント / プラズマ細胞分化 / クロマチン動態変換

Research Abstract

We found that the deposition of histone variant was markedly decreased during B cell differentiation into plasma cells. We also confirmed by chip-seq and mRNA-seq that the histone variant bind to the regulatory element of genes that will be expressed during differentiation. Furthermore, constitutive expression of his ton variant in B cells resulted in the inhibition of plasma cell generation. These findings suggest that B cel differentiation is controlled by dynamic change of chromatin structure.

Research Products

• [Journal Article] STIM1 Controls Neuronal Ca(2+) Signaling, mGluR1-Dependent Synaptic Transmission, and Cerebellar Motor Behavior (2014)
  • Author(s): Hartmann J, Karl RM, Alexander RP, Adelsberger H, Brill MS, Rühlmann C, Ansel A, Sakimura K, Baba Y, Kurosaki T, Misgeld T, Konnerth A.
  • Journal Title: Neuron Volume: 82(3) Pages: 635-644
  • Peer Reviewed
• [Journal Article] Generation of colonic IgA-secreting cells in the caecal patch (2014)
  • Author(s): Masahata K, Umemoto E, Kayama H, Kotani M, Nakamura S, Kurakawa T, Kikuta J, Gotoh K, Motooka D, Sato S, Higuchi T, Baba Y, Kurosaki T, Kinoshita M, Shimada Y, Kimura T, Okumura R, Takeda A, Tajima M, Yoshie O, Fukuzawa M, Kiyono H, Fagarasan S, Iida T, Ishii M, Takeda K.
  • Journal Title: Nat. Commun Volume: 5 Pages: 3704-3704
  • Peer Reviewed
• [Journal Article] Intrinsic disorder mediates cooperative signal transduction in STIM1 (2014)
  • Author(s): Furukawa Y, Teraguchi S, Ikegami T, Dagliyan O, Jin L, Hall D, Dokholyan N, Namba K, Akira S, Kurosaki T, Baba Y, Standley D.
  • Journal Title: J. Mol. Biol Volume: 426(10) Pages: 2082-2097
  • Peer Reviewed
• [Journal Article] The STIM1 calcium sensor is required for activation of the phagocyte oxidase during inflammation and host defense (2014)
  • Author(s): Zhang H, Clemens RA, Liu F, Hu Y, Baba Y, Theodore P, Kurosaki T, Lowell CA.
  • Journal Title: Blood Volume: 123(14) Pages: 2238-2249
  • Peer Reviewed
• [Journal Article] Intrinsic disorder mediates cooperative signal transduction in STIM1 (2014)
  • Author(s): Furukawa, Y., Teraguchi, S., Ikegami, T., Dagliyan, O., Jin, L., Hall, D., Dokholyan, N.V., Namba, K., Akira, S., Kurosaki, T., Baba, Y., Standley, D.M.
  • Journal Title: J Mol Biol Volume: 426 Issue: 10 Pages: 2082-97
  • DOI: 10.1016/j.jmb.2014.03.006
  • Peer Reviewed / Open Access
• [Journal Article] The STIM1 calcium sensor is required for activation of the phagocyte oxidase during inflammation and host defense. (2014)
  • Author(s): Zhang H, Clemens RA, Liu F, Hu Y, Baba Y, Theodore P, Kurosaki T, Lowell CA.
  • Journal Title: Blood Volume: in press
  • Peer Reviewed
• [Journal Article] Calcium signaling in B cells : Regulation of cytosolic Ca^<2+> increase and its sensor molecules, STIM1 and STIM2 (2013)
  • Author(s): Baba Y, Matsumoto M, Kurosaki T.
  • Journal Title: Mol. Immunol
• [Journal Article] Calcium signaling in B cells : Regulation of cytosolic Ca^<2+> increase and its sensor molecules, STIM1 and STIM2 (2013)
  • Author(s): Baba, Y., Matsumoto, M., Kurosaki, T
  • Journal Title: Mol. Immunol Volume: 39 Issue: 2 Pages: 136-147
  • DOI: 10.1016/j.molimm.2013.10.006
  • Peer Reviewed / Open Access
• [Presentation] B細胞におけるSOCEの生理的役割 (2013)
  • Author(s): 馬場義裕、黒崎知博
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船堀,東京
• [Presentation] The mechanism of calcium influx induced by ER calcium sensor STIM and its physiological function (2012)
  • Author(s): 馬場義裕
  • Organizer: 第85回日本生化学学会大会
  • Place of Presentation: 福岡国際会議場,福岡
• [Presentation] Physiological significance for Calcium Influx in B cell (2012)
  • Author(s): 馬場義裕
  • Organizer: 第40回日本臨床免疫学会
  • Place of Presentation: 京王プラザホテル,東京
• [Book] B細胞からのIL-10産生機序, 臨床免疫・アレルギー科(vol. 60(3)) (2013)
  • Author(s): 馬場義裕
• [Book] 制御性B細胞におけるSTIM依存的カルシウム流入の役割, 薬学雑誌(133(4)) (2013)
  • Author(s): 松本真典、馬場義裕
• [Book] 臨床免疫・アレルギー科 (2013)
  • Author(s): 馬場義裕
  • Total Pages: 5
  • Publisher: 科学評論社
• [Book] B細胞のCa^<2+>シグナルと自己免疫性炎症反応の抑制, 医学のあゆみ(vol. 240 (3)) (2012)
  • Author(s): 馬場義裕,黒崎知博
  • Publisher: 医歯薬出版社