Molecular analyses of B-cell memory development through synthetic immunology
| Project/Area Number |
24659225
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Immunology
|
|---|
| Research Institution | Tokyo University of Science |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 免疫応答 / 免疫学的記憶 / 胚中心 / 細胞分化 / B細胞 / 免疫学 / プラズマ細胞 |
|---|
| Research Abstract |
Upon immunization, B cells proliferate to form germinal centers (GC) in the lymphoid organs and differentiate into either long-lived plasma (LP) or memory B (Bmem) cells. Mechanisms for Bmem cell development remain poorly understood, partly due to the lack of an in vitro model system. Recently, we have established a culture system in which naïve B cells undergo massive expansion and isotype switching, and generate GC-like B cells termed iGB cells. The iGB cells after primary IL-4 culture differentiate into functional Bmem-like (iMB) cells in mice, whereas those after the secondary IL-21 culture differentiate into LP but not iMB cells. Thus, this system will facilitate dissection of GC-B cell differentiation programs. In conjunction with knock-out mice, we demonstrated that transcription factor Bach2 and others are required not only for Bmem development. Systematic reconstitution of these factors in iGB cells will allow us to understand the mechanism for Bmem development.
|
Report
(3 results)
Research Products
(20 results)
-
-
-
[Journal Article] Germinal center dysregulation by histone methyltransferase EZH2 promotes lymphomagenesis.2013
Author(s)
Caganova M, Carrisi C, Varano G, Mainoldi F, Zanardi F, Germain PL, George L, Alberghini F, Ferrarini L, Talukder AK, Ponzoni M, Testa G, Nojima T, Doglioni C, Kitamura D,
-
Journal Title
J Clin Invest
Volume: 123
Issue: 12
Pages: 5009-5022
DOI
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] E2A and CBP/p300 act in synergy to promote chromatin accessibility of the immunoglobulin | locus.2012
Author(s)
Sakamoto, S., ffakae, K., Anzai, Y., Murai, K., Tamaki, N., Miyazaki, M., Miyazaki, K., Romanow, W. J., Ikawa, T., Kitamura, D., Yanagihira, I., Minato, N., Murre, C., Agata, Y
-
Journal Title
J. Immunol
Volume: 188
Issue: 11
Pages: 5547-5560
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
