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Development of target-specific multifunctional exosomes loaded with RNA drugs

Research Project

Project/Area Number 24659260
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Applied pharmacology
Research InstitutionKyoto University

Principal Investigator

TAKAHASHI Yuki  京都大学, 薬学研究科(研究院), 助教 (00547870)

Co-Investigator(Kenkyū-buntansha) TAKAKURA Yoshinobu  京都大学, 大学院薬学研究科, 教授 (30171432)
NISHIKAWA Makiya  京都大学, 大学院薬学研究科, 准教授 (40273437)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsドラッグデリバリー / エキソソーム / ルシフェラーゼ / イメージング / DDS
Research Abstract

In the present study, in vivo fate of exosomes was analyzed for the goal of the development of exosome-based drug delivery system. B16BL6 murine melanoma cells were selected as mode exosome-producing cells. It was found that intravenously administered exosomes quickly disappeared from blood circulation with short half-life of approximately 2 minutes. Exosomes distributed mainly to the liver, lung and spleen. In addition, it was demonstrated that quick clearance of exosomes from blood circulation was caused by the uptake of exosomes by macrophages in the liver and spleen.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (14 results)

All 2014 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (12 results) (of which Invited: 1 results)

  • [Journal Article] Visualization and in vivo tracking of the exosomes of murine melanoma B16-BL6 cells in mice after intravenous injection2013

    • Author(s)
      Takahashi Y, Nishikawa M, Shinotsuka H, Matsui Y, Ohara S, Imai T, Takakura Y.
    • Journal Title

      Journal of Biotechnology

      Volume: 165 Pages: 77-84

    • Related Report
      2013 Final Research Report
  • [Journal Article] Visualization and in vivo tracking of the exosomes of murine melanoma B16-BL6 cells in mice after intravenous injection.2013

    • Author(s)
      Takahashi Y, Nishikawa M, Shinotsuka H, Matsui Y, Ohara S, Imai T, Takakura Y.
    • Journal Title

      Journal of Biotechnology

      Volume: 165 Issue: 2 Pages: 77-84

    • DOI

      10.1016/j.jbiotec.2013.03.013

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Streptavidin-biotin結合を利用したexosomeの放射標識によるexosome体内動態定量的解析法の開発2014

    • Author(s)
      森下将輝、高橋有己、佐野紘平、西川元也、佐治英郎、高倉喜信
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本
    • Related Report
      2013 Final Research Report
  • [Presentation] Streptavidin-biotin結合を利用したexosomeの放射標識によるexosome体内動態定量的解析法の開発2014

    • Author(s)
      森下 将輝、高橋 有己、佐野 紘平、西川 元也、佐治 英郎、高倉 喜信
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本大学工学部2号館(熊本市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] xosomeを基盤とするDDS開発を指向した体内動態評価システムの開発2013

    • Author(s)
      高橋有己、西川元也、高倉喜信
    • Organizer
      第29回日本DDS学会学術集会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] Gaussia Luciferase-Lactadherin融合タンパク質を利用したexosomeの体内動態評価法の開発2013

    • Author(s)
      今井貴文、高橋有己、西川元也、高倉喜信
    • Organizer
      第29回日本DDS学会学術集会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] 蛍光標識exosomeを利用した細胞とexosomeとの相互作用解析2013

    • Author(s)
      加藤佳奈、大原早織、高橋有己、西川元也、高倉喜信
    • Organizer
      第29回日本DDS学会学術集会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] Gaussia Luciferaseを利用したエクソソーム標識法の開発によるエクソソーム体内動態解析2013

    • Author(s)
      今井貴文、高橋有己、西川元也、高倉喜信
    • Organizer
      日本薬剤学会第28年会
    • Place of Presentation
      愛知
    • Related Report
      2013 Final Research Report
  • [Presentation] exosomeを基盤とするDDS開発を指向した体内動態評価システムの開発2013

    • Author(s)
      高橋有己、西川元也、高倉喜信
    • Organizer
      第29回日本DDS学会学術集会
    • Place of Presentation
      京都テルサ(京都市南区)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 今井貴文、高橋有己、西川元也、高倉喜信Gaussia Luciferase-Lactadherin 融合タンパク質を利用したexosomeの体内動態評価法の開発2013

    • Author(s)
      今井貴文、高橋有己、西川元也、高倉喜信
    • Organizer
      第29回日本DDS学会学術集会
    • Place of Presentation
      京都テルサ(京都市南区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 蛍光標識exosomeを利用した細胞とexosomeとの相互作用解析2013

    • Author(s)
      加藤佳奈、大原早織、高橋有己、西川元也、高倉喜信
    • Organizer
      第29回日本DDS学会学術集会
    • Place of Presentation
      京都テルサ(京都市南区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Gaussia Luciferaseを利用したエクソソーム標識法の開発によるエクソソーム体内動態解析2013

    • Author(s)
      今井貴文、高橋有己、西川元也、高倉喜信
    • Organizer
      日本薬剤学会第28年会
    • Place of Presentation
      ウインクあいち(名古屋市中村区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Cellular Uptake Mechanism of Fluorescently Labeled Exosomes2012

    • Author(s)
      Saori Ohara, Yuki Takahashi, Makiya Nishikawa, Yoshinobu Takakura.
    • Organizer
      第6回次世代を担う若手医療薬科学シンポジウム
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] Cellular Uptake Mechanism of Fluorescently Labeled Exosomes.2012

    • Author(s)
      Saori Ohara, Yuki Takahashi, Makiya Nishikawa, Yoshinobu Takakura.
    • Organizer
      第6回次世代を担う若手医療薬科学シンポジウム
    • Place of Presentation
      京都大学薬学部(京都府)
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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