A new culture method using bacteria-adhesive particles
| Project/Area Number |
24659266
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 細菌培養 / intestinal microbiota / culture |
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| Research Abstract |
We investigated changes of bacterial composition before and after 3 days culture of fecal bacteria under the aerobic or acidic condition by the molecular methods. A part of intestinal bacteria was able to grow in Hank's balanced salt solution containing 10% fetal calf serum under the aerobic condition. As was expected we observed the significant increase of facultative anaerobes and decrease of obligate anaerobes. However, Bacteroides fragilis, an anaerobe, could regrow after 7 days aerobic culture. Terminal restriction length polymorphism analysis demonstrated the decreased number of peaks after the aerobic culture, suggesting loss of bacterial diversity. The maintenance at pH 4.5 in cultures resulted in the inhibition of bacterial growth, suggesting possible contribution of pH control to prevention of bacterial infection.
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Report
(3 results)
Research Products
(26 results)
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[Journal Article] Long-term Incidence and Characteristics of Intestinal Failure in Crohn’s disease: A Multicenter Study2014
Author(s)
Watanabe K, Sasaki I, Fukushima K, Futami K, Ikeuchi H, Sugita A, Nezu R, Mizushima T, Kameoka S, Kusunoki M, Yoshioka K, Funayama Y, Watanabe T, Fujii H, Watanabe M
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Journal Title
Journal of Gastroenterology
Volume: 49
Issue: 2
Pages: 231-238
DOI
Related Report
Peer Reviewed
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[Journal Article] 炎症性腸疾患の経過潰瘍性大腸炎の術後経過2013
Author(s)
渡辺和宏、柴田近,小川仁,長尾宗紀,羽根田祥,工藤克昌,神山篤史,鈴木秀幸,三浦康,内藤剛,鹿郷昌之,田中直樹,大沼忍,佐々木宏之,海野倫明,福島浩平
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Journal Title
胃と腸
Volume: 48
Pages: 731-736
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[Journal Article] A shift from colon- to ileum-predominant bacteria in ileal-pouch feces following total proctocolectomy2012
Author(s)
Hinata M, Kohyama A, Ogawa H, Haneda S, Watanabe K, Suzuki H, Shibata C, Funayama Y, Takahashi K, Sasaki I, Fukushima K.
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Journal Title
Dig Dis Sci
Volume: 57(11)
Issue: 11
Pages: 2965-74
DOI
Related Report
Peer Reviewed
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[Presentation] Surgical Site Infection予防最新プラクティス炎症性腸疾患手術例における切開創手術部位感染予防有効な予防策と問題点の検討2013
Author(s)
髙橋賢一,舟山裕士,徳村弘実,豊島隆,武者宏昭,西條文人,松村直樹,野村良平,武藤満完,安本明浩,松村勝,山田佳緒里,福島浩平,小川仁,羽根田祥,渡辺和宏,鈴木秀幸,佐々木巌
Organizer
第112回日本外科学会
Place of Presentation
福岡
Year and Date
2013-11-28
Related Report
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[Presentation] 潰瘍性大腸炎に対する多施設共同手術部位感染症サーベイランス2013
Author(s)
荒木俊光,大北喜基,内野基,池内浩基,佐々木巌,舟山裕士,福島浩平,二見喜太郎,前田清,飯合恒夫,板橋道朗,小林美奈子,毛利靖彦,楠正人
Organizer
第26回日本外科感染症学会
Place of Presentation
神戸
Year and Date
2013-11-26
Related Report
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[Presentation] ともに大腸全摘に至った重症潰瘍性大腸炎の父子例2013
Author(s)
虻川大樹,村山晶俊,梅林宏明,阿部弘,稲垣徹史,三浦克志,天江新太郎,佐藤智行,中村恵美,武山淳二,福島浩平,武田弘明,磯部秀樹
Organizer
第40回日本小児栄養消化器肝臓学会
Place of Presentation
東京
Year and Date
2013-11-01
Related Report
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[Presentation] Crohn病症例の大腸(亜)全摘術におけるHALSの適応と成績2012
Author(s)
高橋賢一,舟山裕士,生澤史江,徳村弘実,豊島隆,武者宏昭,西條文人,松村直樹,武藤満完,安本明浩,福島浩平,小川仁,羽根田祥,渡辺和宏,鈴木秀幸,佐々木巖
Organizer
第25回日本内視鏡外科学会
Place of Presentation
横浜
Year and Date
2012-12-06
Related Report
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[Presentation] 炎症性腸疾患手術例に対する術前の完全静脈栄養の栄養アセスメント蛋白改善効果に関する検討2012
Author(s)
高橋賢一,舟山裕士,生澤史江,徳村弘実,豊島隆,武者宏昭,西條文人,鈴木洋,松村直樹,武藤満完,安本明浩,又吉信貴,澤田健太郎,柴原みい,福島浩平,小川仁,渡辺和宏,羽根田祥,鈴木秀幸,佐々木巖
Organizer
第49回日本外科代謝栄養学会
Place of Presentation
浦安
Year and Date
2012-07-05
Related Report
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