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Establishment of new extraction system from bone marrow smear by laser microdissection

Research Project

Project/Area Number 24659273
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionKyoto University

Principal Investigator

ADACHI Souichi  京都大学, 医学(系)研究科(研究院), 教授 (10273450)

Co-Investigator(Renkei-kenkyūsha) ITOU Hiroshi  京都大学, 医学研究科, 助教 (20362387)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords遺伝子解析 / 血液標本 / 白血病 / 骨髄異形成症候群 / 新規予後因子
Research Abstract

We established a new laser microdissection system extracting cDNA and mRNA of leukemic blasts from smear of bone marrow samples of transient abnormal myelopoiesis (TAM) or acute myeloid leukemia with Down syndrome (AML-DS). We could detect same GATA1 mutation in the TAM patient both from bone marrow samples and from the microdissecton system. We could also detect GAPDH from mRNA through this microdissection system and measured CXCR4 expression by RT-PCR. We will be able to detect new prognostic factors from AML or MDS patients by next generation sequencing through this microdissection system.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (29 results)

All 2013 2012 Other

All Journal Article (11 results) (of which Peer Reviewed: 5 results) Presentation (10 results) (of which Invited: 3 results) Book (4 results) Remarks (4 results)

  • [Journal Article] Excess treatment reduction including anthracyclines results in higher incidence of relapse in core binding factor acute myeloid leukemia in children2013

    • Author(s)
      Tomizawa D, Adachi S, et al.
    • Journal Title

      Leukemia

      Volume: 27(12) Pages: 2413-2416

    • Related Report
      2013 Final Research Report
  • [Journal Article] Appropriate dose reduction in induction therapy is essential for the treatment of infants with acute myeloid leukemia : a report from the Japanese Pediatric Leukemia/Lymphoma Study Group2013

    • Author(s)
      Tomizawa D, Adachi S, et al.
    • Journal Title

      Int J Haematol

      Volume: 98(5) Pages: 578-588

    • Related Report
      2013 Final Research Report
  • [Journal Article] Excess treatment reduction including anthracyclines results in higher incidence of relapse in core binding factor acute myeloid leukemia in children.2013

    • Author(s)
      Tomizawa D, Tawa A, Watanabe T, Moriya-Saito A, Kudo K, Taga T, Iwamoto S, Shimada A, , Terui K, Moritake H, Kinoshita A, Takahashi H, Nakayama H, Koh K, Kigasawa H, Kosaka Y, Miyachi H, Horibe K, Nakahata T, and Adachi S.
    • Journal Title

      Leukemia

      Volume: 27 Issue: 12 Pages: 2413-2416

    • DOI

      10.1038/leu.2013.153

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Appropriate dose reduction in induction therapy is essential for the treatment of infants with acute myeloid leukemia: a report from the Japanese Pediatric Leukemia/Lymphoma Study Group.2013

    • Author(s)
      Tomizawa D, Tawa A, Watanabe T, Saito AM, Kudo K, Taga T, Iwamoto S, Shimada A, Terui K, Moritake H, Kinoshita A, Takahashi H, Nakayama H, Kiyokawa N, Isoyama K, Mizutani S, Hara J, Horibe K, Nakahata T, Adachi S.
    • Journal Title

      Int J Hematol

      Volume: 98 Pages: 578-588

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DMNT3A mutations are rare in childhood acute myeloid leukaemia, myelodysplastic syndromes and juvenile myelomonocytic leukaemia2012

    • Author(s)
      Shiba N, Adachi S, et al.
    • Journal Title

      Brit J Haematol

      Volume: 156(3) Pages: 413-414

    • Related Report
      2013 Final Research Report
  • [Journal Article] High WT1 mRNA expression after induction chemotherapy and FLT3-ITD have prognostic impact in pediatric acute myeloid leukemia : a study of the Japanese Childhood AML Cooperative Study Group2012

    • Author(s)
      Shimada A, Adachi S, et al.
    • Journal Title

      Int J Haematol

      Volume: 96(4) Pages: 469-476

    • NAID

      10031126738

    • Related Report
      2013 Final Research Report
  • [Journal Article] An inv(16)(p13.3;q24.3)-encoded CBFA2T3-GLIS2 fusion protein defines an aggressive subtype of pediatric acute megakaryoblastic leukemia2012

    • Author(s)
      Gruber TA, Adachi S, et al.
    • Journal Title

      Cancer Cell

      Volume: 22(5) Pages: 683-697

    • Related Report
      2013 Final Research Report
  • [Journal Article] DNMT3A mutations are rare in childhood acute myeloid leukaemia, myelodysplastic syndromes and juvenile myelomonocytic leukaemia2012

    • Author(s)
      Shiba N, Taki T, Park MJ, Shimada A, Sotomatsu M, Adachi S, Tawa A, Horibe K, Tsuchida M, Hanada R, Tsukimoto I, Arakawa H, Hayashi Y.
    • Journal Title

      Br J Haematol

      Volume: 156 Issue: 3 Pages: 413-414

    • DOI

      10.1111/j.1365-2141.2011.08879.x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] High WT1 mRNA expression after induction chemotherapy and FLT3-ITD have prognostic impact in pediatric acute myeloid leukemia: a study of the Japanese Childhood AML Cooperative Study Group2012

    • Author(s)
      Shimada A, Taki T, Koga D, Tabuchi K, Tawa A, Hanada R, Tsuchida M, Horibe K, Tsukimoto I, Adachi S, Kojima S, Hayashi Y
    • Journal Title

      Int J Hematol

      Volume: 96 Issue: 4 Pages: 469-476

    • DOI

      10.1007/s12185-012-1163-1

    • NAID

      10031126738

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] An Inv(16)(p13.3q24.3)-Encoded CBFA2T3-GLIS2 Fusion Protein Defines an Aggressive Subtype of Pediatric Acute Megakaryoblastic Leukemia2012

    • Author(s)
      Gruber TA, Larson Gedman A, Zhang J, Koss CS, Marada S, Ta HQ, Chen SC, Su X, Ogden SK, Dang J, Wu G, Gupta V, Andersson AK, Pounds S, Shi L, Easton J, Barbato MI, Mulder HL, Manne J, Wang J, Rusch M, Ranade S, Ganti R, Parker M, Ma J, Radtke I, Ding L, Cazzaniga G, Biondi A, Kornblau SM, Ravandi F, Kantarjian H, Nimer SD, Dohner K, Dohner H, Ley TJ, Ballerini P, Shurtleff S, Tomizawa D, Adachi S, Hayashi Y, Tawa A, Shih LY, Liang DC, Rubnitz JE, Pui CH, Mardis ER, Wilson RK, Downing JR.
    • Journal Title

      Cancer Cell

      Volume: 22 Issue: 5 Pages: 683-697

    • DOI

      10.1016/j.ccr.2012.10.007

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Prognostic implications of CEBPA mutations in pediatric acute myeloid leukemia : A report from the Japanese Pediatric Leukemia/Lymphoma Study Group

    • Author(s)
      Matsuo H, Itoh H, Adachi S, et al.
    • Journal Title

      Blood Cancer Journal

      Volume: (in press)

    • Related Report
      2013 Final Research Report
  • [Presentation] 血液疾患の予後因子をスメア標本より検出するシステムの確立2013

    • Author(s)
      松尾英将、池内絢香、志賀修一、土岐力、照井君典、伊藤悦朗、足立壮一
    • Organizer
      第8回日本臨床検査学教育学会学術大会
    • Place of Presentation
      大阪市
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] JPLSG studies for AML2013

    • Author(s)
      Adachi S
    • Organizer
      St. Jude Goround Rounds
    • Place of Presentation
      Memphis, USA
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] JPLSG studies for AML2012

    • Author(s)
      Adachi S.
    • Organizer
      St. Jude Ground Rounds
    • Place of Presentation
      Memphis, USA
    • Year and Date
      2012-12-11
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] GATA2 muitations in Pediatric Acute Myeloid Leukemia : A Study of the Japanese Childhood AML Cooperative Study Group. th Japanese Childhood AML Cooperative Study Group. Int J Haematol2012

    • Author(s)
      Shiba N, Adachi S, et al.
    • Organizer
      American Society of Hematology 54 Annual Meeting
    • Place of Presentation
      Atlanta, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] NUP98-NSD1 Related Gene Expression Signature is Strongly Associated with a Poor Prognosis in Pediatric Acute Myeloid Leukemia2012

    • Author(s)
      Shiba N, Adachi S, et al.
    • Organizer
      American Society of Hematology 54^<th> Annual Meeting
    • Place of Presentation
      Atlanta, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Genetic Basis of Myeloid Proliferation Related to Down Syndrome2012

    • Author(s)
      Yoshida K, Adachi S, et al.
    • Organizer
      American Society of Hematology 54^<th> Annual Meeting
    • Place of Presentation
      Atlanta, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] JPLSG studies for AML..2012

    • Author(s)
      Adachi S
    • Organizer
      St. Jude Ground Rounds
    • Place of Presentation
      Memphis, USA
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] GATA2 Mutations in Pediatric Acute Myeloid Leukemia: A Study of the Japanese Childhood AML Cooperative Study Group2012

    • Author(s)
      Shiba N, Adachi S, et al.
    • Organizer
      American Society of Hematology 54rd Annual Meeting
    • Place of Presentation
      Atlanta, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] NUP98-NSD1 Related Gene Expression Signature Is Strongly Associated with a Poor Prognosis in Pediatric Acute Myeloid Leukemia2012

    • Author(s)
      Shiba N, Adachi S et al.
    • Organizer
      American Society of Hematology 54rd Annual Meeting
    • Place of Presentation
      Atlanta, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Genetic Basis of Myeloid Proliferation Related to Down Syndrome.2012

    • Author(s)
      Yoshida K, Adachi S, et al.
    • Organizer
      American Society of Hematology 54rd Annual Meeting
    • Place of Presentation
      Atlanta, USA
    • Related Report
      2012 Research-status Report
  • [Book] 医療のための血液がんの標準的化学療法2013

    • Author(s)
      足立壮一直江知樹、堀部敬三監修チーム
    • Publisher
      メディカル・サイエンスインターナショナル社
    • Related Report
      2013 Final Research Report
  • [Book] プリンシプル血液疾患の臨床ここまできた白血病/MDS治療2013

    • Author(s)
      足立壮一金倉譲、松村到編集
    • Publisher
      中山書店
    • Related Report
      2013 Final Research Report
  • [Book] チーム医療のための血液がんの標準的化学療法2013

    • Author(s)
      足立壮一/直江知樹、堀部敬三監修
    • Publisher
      メディカル・サイエンス・インターナショナル社
    • Related Report
      2013 Annual Research Report
  • [Book] プリンシプル血液疾患の臨床 ここまできた白血病/MDS治療2013

    • Author(s)
      足立壮一/金倉譲、松村到編集
    • Publisher
      中山書店
    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://www.med.kyoto-u.ac.jp/organization-staff/research/human_health/mt0302/

    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://adachilab.web.fc2.com/member.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 血液・生体防御学研究室

    • URL

      http://www.med.kyoto-u.ac.jp/organization-staff/research/human_health/mt0302/

    • Related Report
      2012 Research-status Report
  • [Remarks] 血液・生体防御学研究室

    • URL

      http://adachilab.web.fc2.com/index.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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