• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Screening of novel anti-citrullinated protein antibodies for rheumatoid arrhtiris by protein array

Research Project

Project/Area Number 24659274
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionKyoto University

Principal Investigator

OHMURA Koichiro  京都大学, 医学(系)研究科(研究院), 講師 (40432372)

Co-Investigator(Renkei-kenkyūsha) SAWASAKI Tatsuya  愛媛大学, 無細胞生命科学工学研究センター, 准教授 (50314969)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords免疫血清学 / 抗CCP抗体 / ACPA / 関節リウマチ / バイオマーカー / 抗CCP抗体陰性RA / AlphaScreen法 / 蛋白アレイ / 新規自己抗体 / AlphaScreen
Research Abstract

Anti-cyclic citrullinated peptide (CCP) antibody is a specific autoantibody against rheumatoid arthritis (RA). However, it becomes positive in only about 50% of early RA patients, and sometimes it is difficult to correctly diagnose RA in the early phase. Therefore, the diagnostic biomarkers for anti-CCP antibody-negative RA have been required. We screened the anti-CCP antibody-negative RA sera for novel anti-citrullinated protein antibodies (ACPA) using AlphaScreen method. We found 14 novel anti-citrullinated protein antibodies from over 2,000 citrullinated proteins, out of which 2 were assured by Western blotting and ELISA.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (24 results)

All 2014 2013 2012 Other

All Journal Article (15 results) (of which Peer Reviewed: 15 results,  Open Access: 1 results) Presentation (9 results) (of which Invited: 1 results)

  • [Journal Article] Inverse association between air pressure and rheumatoid arthritis synovitis: an observational study2014

    • Author(s)
      Terao C, Hashimoto M, Furu M, Nakabo S, Ohmura K, Nakashima R, Imura Y, Yukawa N, Yoshifuji H, Matsuda F, Ito H, Fujii T, Mimori T.
    • Journal Title

      PLoS One

      Volume: 9 Issue: 1 Pages: e85376-e85376

    • DOI

      10.1371/journal.pone.0085376

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome-Wide Association Study and Gene Expression Analysis Identifies CD84 as a Predictor of Response to Etanercept Therapy in Rheumatoid Arthritis2013

    • Author(s)
      Cui J, Stahl EA, Ohmura K, Plenge R, et al.
    • Journal Title

      PLoS Genet

      Volume: 9

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neutrophils are essential as a source of IL-17 in the effector phase of arthritis2013

    • Author(s)
      Katayama M, Ohmura K, Yukawa N, Terao C, Hashimoto M, Yoshifuji H, Kawabata D, Fujii T, Iwakura Y, Mimori T.
    • Journal Title

      PLos One

      Volume: 8

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neutrophils are essential as a source of IL-17 in the effector phase of arthritis.2013

    • Author(s)
      Katayama M, Ohmura K, Yukawa N, Terao C, Hashimoto M, Yoshifuji H, Kawabata D, Fujii T, Iwakura Y, Mimori T
    • Journal Title

      PLos One

      Volume: 8(5) Issue: 5 Pages: e62231-e62231

    • DOI

      10.1371/journal.pone.0062231

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome-Wide Association Study and Gene Expression Analysis Identifies CD84 as a Predictor of Response to Etanercept Therapy in Rheumatoid Arthritis.2013

    • Author(s)
      Cui J, Stahl EA, Saevarsdottir S, Miceli C, Diogo D, Trynka G, Raj T, Mirkov MU, Canhao H, Ikari K, Terao C, Okada Y, Wedrén S, Askling J, Yamanaka H, Momohara S, Taniguchi A, Ohmura K, Matsuda F, Mimori T, Gupta N, Kuchroo M, Morgan AW, Isaacs JD, Wilson AG, Hyrich KL, Herenius M, Doorenspleet ME, Tak PP, et al
    • Journal Title

      PLoS Genet

      Volume: 9 Issue: 3 Pages: e1003394-e1003394

    • DOI

      10.1371/journal.pgen.1003394

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three groups in the 28 joints for rheumatoid arthritis synovitis--analysis using more than 17,000 assessments in the KURAMA database2013

    • Author(s)
      Terao C, Hashimoto M, Yamamoto K, Murakami K, Ohmura K, Nakashima R, Yamakawa N, Yoshifuji H, Yukawa N, Kawabata D, Usui T, Yoshitomi H, Furu M, Yamada R, Matsuda F, Ito H, Fujii T, Mimori T
    • Journal Title

      PLoS One

      Volume: 8(3) Issue: 3 Pages: e59341-e59341

    • DOI

      10.1371/journal.pone.0059341

    • NAID

      120005244457

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PLD4 as a novel susceptibility gene for systemic sclerosis in a Japanese population2013

    • Author(s)
      Terao C, Ohmura K, Kawaguchi Y, Nishimoto T, Kawasaki A, Takehara K, Furukawa H, Kochi Y, Ota Y, Ikari K, Sato S, Tohma S, Yamada R, Yamamoto K, Kubo M, Yamanaka H, Kuwana M, Tsuchiya N, Matsuda F, Mimori T
    • Journal Title

      Arthritis Rheum

      Volume: 65 Issue: 2 Pages: 472-480

    • DOI

      10.1002/art.37777

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Functional Variants in NFKBIE and RTKN2 Involved in Activation of the NF-κB Pathway Are Associated with Rheumatoid Arthritis in Japanese2012

    • Author(s)
      Myouzen K, Kochi Y, Okada Y, Terao C, Ohmura K et al.
    • Journal Title

      PLoS Genet

      Volume: 8(9)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Recent advances on the genetics of rheumatoid arthritis : current topics and the future2012

    • Author(s)
      Ohmura K, Terao C, Mimori T.
    • Journal Title

      Inflamm Regen

      Volume: 32 (3) Pages: 90-98

    • NAID

      130004943811

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] ACPA-negative RA consists of two genetically distinct subsets based on RF positivity in Japanese2012

    • Author(s)
      Terao C, Ohmura K, Ikari K, Kochi Y, et al.
    • Journal Title

      PLoS One

      Volume: 7(7)

    • NAID

      120004754351

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Meta-analysis identifies nine new loci associated with rheumatoid arthritis in the Japanese population2012

    • Author(s)
      Okada Y, Terao C, Ikari K, Kochi Y, Ohmura K, et al.
    • Journal Title

      Nat Genet

      Volume: 44(5) Pages: 511-6

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Meta-analysis identifies nine new loci associated with rheumatoid arthritis in the Japanese population2012

    • Author(s)
      Okada Y, Terao C, Ikari K, Kochi Y, Ohmura K, Suzuki A, Kawaguchi T, Stahl EA, Kurreeman FA, Nishida N, Ohmiya H, Myouzen K, Takahashi M, Sawada T, Nishioka Y, Yukioka M, Matsubara T, Wakitani S, Teshima R, Tohma S, Takasugi K, Shimada K, Murasawa A, Honjo S, Matsuo K, Tanaka H, Tajima K, Suzuki T, Iwamoto T, Kawamura Y, Tanii H, Okazaki Y, Sasaki T, Gregersen PK, Padyukov L, Worthington J, Siminovitch KA, Lathrop M, Taniguchi A, Takahashi A, Tokunaga K, Kubo M, Nakamura Y, Kamatani N, Mimori T, Plenge RM, Yamanaka H, Momohara S, Yamada R, Matsuda F, Yamamoto K.
    • Journal Title

      Nat Genet.

      Volume: (in press) Issue: 5 Pages: 511-6

    • DOI

      10.1038/ng.2231

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] ACPA-negative RA consists of two genetically distinct subsets based on RF positivity in Japanese.2012

    • Author(s)
      Terao C, Mimori T, et al (21人中21人目)
    • Journal Title

      PLoS One

      Volume: 7 Issue: 7 Pages: e40067-e40067

    • DOI

      10.1371/journal.pone.0040067

    • NAID

      120004754351

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Recent advances on the genetics of rheumatoid arthritis: current topics and the future.2012

    • Author(s)
      Ohmura K, Terao C, Mimori T.
    • Journal Title

      Inflamm Regen

      Volume: 32 Pages: 90-98

    • NAID

      130004943811

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Functional variants in NFKBIE and RTKN2 involved in activation of the NF-κB pathway are associated with rheumatoid arthritis in Japanese.2012

    • Author(s)
      Myouzen K, Kochi Y, Okada Y, Terao C, Suzuki A et al.
    • Journal Title

      PLoS Genet

      Volume: 8 Issue: 9 Pages: e1002949-e1002949

    • DOI

      10.1371/journal.pgen.1002949

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Clinical characteristics of rheumatoid factor-positive or–negative subsets of anti-citrullinated peptide/protein antibody-negative rheumatoid arthritis2013

    • Author(s)
      Hiwa R, Ohmura K, Mimori T, et al.
    • Organizer
      American College of Rheumatology Annual Scientific Meeting 2013
    • Place of Presentation
      San Diego(poster presentation)
    • Year and Date
      2013-10-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Only rheumatoid factor-positive subset of anti-citrullinated peptide/protein antibody-negative rheumatoid arthritis seroconverts to anti-citrullinated peptide/protein antibody positive2013

    • Author(s)
      Hiwa R, Ohmura K, Mimori T, et al.
    • Organizer
      American College of Rheumatology Annual Scientific Meeting 2013
    • Place of Presentation
      San Diego(poster presentation)
    • Year and Date
      2013-10-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Does anti-cyclic citrullinated peptide (CCP) antibody-negative rheumatoid arthritis (RA) become anti-CCP positive RA?2013

    • Author(s)
      Hiwa R, Ohmura K, Mimori T, et al.
    • Organizer
      第57回日本リウマチ学会総会・学術集会
    • Place of Presentation
      (京都)国際ワークショップ(oral presentation)
    • Related Report
      2013 Final Research Report
  • [Presentation] ACPA-NEGATIVE RHEUMATOID ARTHRITIS CONSISTS OF TWO GENETICALLY DISTINCT SUBSETS BASED ON RF POSITIVITY2012

    • Author(s)
      Terao C, Ohmura K, Mimori T, et al.
    • Organizer
      European League Against Rheumatism 2012
    • Place of Presentation
      Berlin(poster presentation)
    • Related Report
      2013 Final Research Report
  • [Presentation] 抗CCP抗体陰性関節リウマチに関連する遺伝因子2012

    • Author(s)
      大村浩一郎
    • Organizer
      第56回日本リウマチ学会総会・学術集会
    • Place of Presentation
      東京(品川)シンポジウム(oral presentation)
    • Related Report
      2013 Final Research Report
  • [Presentation] Only rheumatoid factor-positive subset of anti-citrullinated peptide/protein antibody-negative rheumatoid arthritis seroconverts to anti-citrullinated peptide/protein antibody positive.

    • Author(s)
      Hiwa R, Ohmura K, Mimori T, et al
    • Organizer
      American College of Rheumatology Annual Scientific Meeting 2013
    • Place of Presentation
      San Diego
    • Related Report
      2013 Annual Research Report
  • [Presentation] Clinical characteristics of rheumatoid factor-positive or –negative subsets of anti-citrullinated peptide/protein antibody-negative rheumatoid arthritis.

    • Author(s)
      Hiwa R, Ohmura K, Mimori T, et al
    • Organizer
      American College of Rheumatology Annual Scientific Meeting 2013
    • Place of Presentation
      San Diego
    • Related Report
      2013 Annual Research Report
  • [Presentation] Does anti-cyclic citrullinated peptide (CCP) antibody-negative rheumatoid arthritis (RA) become anti-CCP positive RA?

    • Author(s)
      Hiwa R, Ohmura K, Mimori T, et al
    • Organizer
      第57回日本リウマチ学会総会・学術集会
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] 抗CCP抗体陰性関節リウマチに関連する遺伝因子

    • Author(s)
      大村 浩一郎
    • Organizer
      第56回日本リウマチ学会総会・学術集会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
    • Invited

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi