Development of a novel method to detect circulating tumor cells (CTCs) by expression of tissue specific mRNAs in peripheral blood.

Research Project

Project/Area Number	24659277
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Laboratory medicine
Research Institution	Takarazuka University
Principal Investigator	TATSUMI Ke-ita 宝塚大学, 看護学部, 教授 (00222109)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000) Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	LAMP / CTC / LAMP法 / 循環腫瘍細胞 / 遺伝子増幅
Outline of Final Research Achievements	To detect circulating tumor cells (CTCs) by expression of tissue specific mRNAs in peripheral blood using Loop-mediated isothermal amplification method (LAMP), expression of thyroid specific genes were studied. Low level expression of mRNAs of one thyroid specific gene was detected by LAMP after reverse transcription (RT-LAMP) in total RNAs from leukocytes. Thus measuring expression levels of tissue specific mRNAs in whole peripheral blood using RT-LAMP may be able to detect CTCs in peripheral blood.