| Project/Area Number |
24659280
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Ehime University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MIYAZAKI Tatsuhiko 岐阜大学, 医学部附属病院病理部, 臨床教授 (80239384)
TAKEMORI Nobuaki 愛媛大学, プロテオサイエンスセンター, 講師 (40533047)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 臨床検査システム / shedding / タンパク質絶対定量 / プロテオーム解析 / プロテオミクス / shedding定量 / 無細胞タンパク質合成 / 炎症マーカー / 蛋白質絶対定量 / 炎症 / MRM / 無細胞蛋白質合成 |
|---|
| Research Abstract |
Ectodomain shedding of transmembrane proteins is induced by inflammatory factors such as chemokines and cytokines. In order to monitor the progress of chronic inflammation, we attempted to establish a monitoring system of the ectodomain shedding using the combination of an originally synthesized internal standard protein mixture and MRM by mass spectrometry. Then, we successfully accomplished the absolute measurement of transmembrane protein contents in mouse tissues at f-mol levels.
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